Investigating prevalence of pathogenic genes (ETA and TSST-1) in Staphylococcus aureus isolated from different wards of the hospitals by PCR method

  • Authors

    • Rashid Ramazanzadeh Cellular & Molecular Research Center and Microbiology Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj- Iran
    • Hadi Mohammadi Talvar Islamic Azad University, Science and Research Campus, Kurdistan branch, Sanandaj, Iran.
    • Mahdi Mirzaii Faculty of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
    • Seyed Sajjad Hasheminasab MSC of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran
    • Hanar Narenji Department of Microbiology, School of Medicine Science, Sanandaj, Iran.
    2015-09-05
    https://doi.org/10.14419/ijsw.v3i2.5168
  • TSST-1, ETA, Staphylococcus aureus, PCR Method, Penicillin.

  • Abstract

    Staphylococcus aureus is the most common pathogenic organisms in the hospitals and communities' infections. It is responsible for more than 80 percent of infectious diseases. The purpose of the present paper is to determine the incidence of Staphylococcus pathogenic genes isolated from different wards of hospitals by PCR method. This study included 61 Staphylococcus aureusisolates collected from different wards hospital, between 2011 and 2012 in University of Kurdistan (Toohhid and Besat hospitals). All isolates were previously identified as Staphylococcus aureusby a standard microbiological procedure. It isolates were incubated at 37ÄŠ for 24h on blood agar; single colonies were tested with tube and slide coagulase, catalase tests and growth on Manito salt agar. Following genomic DNA extraction, the presence of ETA, TSST-1 genes was analyzed by PCR.61 strains of Staphylococcus aureushave been isolated from different wards of the hospital. Frequency of tst gene was 81% and eta gene was 47%. Moreover, frequency of strains with both eta and tst genes was 40%. Results of the present paper indicate that the prevalence of Staphylococcusaureus results on prevalence of eta and tst genes, and this is a matter of concern.

  • References

    1. [1] Casey, A., Lambert, PA, Elliott, T. Staphylococci. (2007). Int J Antimicrob Agents; 29:S23-S32. http://dx.doi.org/10.1016/S0924-8579(07)72175-1.

      [2] Fueyo, J., Mendoza, MC, Rodicio, MR., Muniz, J., Alvarez, M, Martín, MC. Cytotoxin and pyrogenic toxin superantigen gene profiles of Staphylococcus aureus associated with subclinical mastitis in dairy cows and relationships with macrorestriction genomic profiles. (2005). J Clin Microbiol; 43:1278-1284. http://dx.doi.org/10.1128/JCM.43.3.1278-1284.2005.

      [3] Omoe, K., Hu, DL. Takahashiâ€Omoe, H., Nakane, A, Shinagawa, K. Comprehensive analysis of classicaland newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates. (2005). FEMS Microbiol Lett; 246:191-198. http://dx.doi.org/10.1016/j.femsle.2005.04.007.

      [4] Sindhu, N., Sharma, A, Jain, V. Coagulase gene based molecular detection of Staphylococcus aureus directly frommastitic milk samples of Murrah buffalo. (2010). Buffalo Bull; 29:52-59.

      [5] Chapaval, L., Moon, D., Gomes, J., Duarte, F, Tsai, S. An alternative method for Staphylococcus aureus DNA isolation. (2008). Arq Bras Med Vet Zootec; 60:299-306. http://dx.doi.org/10.1590/S0102-09352008000200004.

      [6] Deurenberg, R., Vink, C., Kalenic, S., Friedrich, A., Bruggeman, C, Stobberingh, E. The molecular evolution of methicillinâ€resistant Staphylococcus aureus. (2007). Clin Microbiol Infect; 13:222-235. http://dx.doi.org/10.1111/j.1469-0691.2006.01573.x.

      [7] El-Ghodban, A., Ghenghesh, KS., Márialigeti, K., Esahli, H, Tawil, A. PCR detection of toxic shock syndrome toxin of Staphylococcus aureus from Tripoli, Libya. (2006). J Med Microbiol; 55:179-182. http://dx.doi.org/10.1099/jmm.0.46162-0.

      [8] Abimanyu, N., Murugesan, S, Krishnan, P. High Prevalence of Exfoliative Toxins Among Carrier Isolates of Staphylococcus aureus from Healthy Individuals from Various Communities in Chennai, South India. (2013). Indian J Microbiol; 53:288-290. http://dx.doi.org/10.1007/s12088-013-0360-9.

      [9] Mahon, C., Manuselis, G, Lehman, D. Textbook of Diagnostic Microbiology, Saunders. ISBN 0-7216-7917-X; 2000.

      [10] Wang, S-C., Wu, C-M., Xia, S-C., Qi, Y-H., Xia, L-N, Shen, J-Z. Distribution of superantigenic toxin genes in< i> Staphylococcus aureus</i> isolates from milk samples of bovine subclinical mastitis cases in two major diary production regions of China. (2009). Veterinary microbiology; 137:276-281. http://dx.doi.org/10.1016/j.vetmic.2009.01.007.

      [11] Drews, TD, Temte, JL, Fox, BC. Community-associated methicillin-resistant Staphylococcus aureus: review of an emerging public health concern. (2006). WMJ-MADISON-; 105:52.

      [12] Maniatis, T., Fritsch, EF, Sambrook, J. Molecular cloning: a laboratory manual: Cold Spring Harbor Laboratory Cold Spring Harbor, NY; 1982.

      [13] Mehrotra, M., Wang, G, Johnson, WM. Multiplex PCR for Detection of Genes forStaphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1, and Methicillin Resistance. (2000). J Clin Microbiol; 38:1032-1035.

      [14] Johnson, W., Tyler, S., Ewan, E., Ashton, F., Pollard, D, Rozee, K. Detection of genes for enterotoxins, exfoliative toxins, and toxic shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction. (1991). J Clin Microbiol; 29:426-430.

      [15] Adwan, GM., Abu-Shanab, B., Adwan, K, Jarrar, N. Toxigenicity of Staphylococcus aureus isolates from Northern Palestine. (2006). Emirates Medical Journal; 24:127.

      [16] Udo, EE, Al-Mufti, S, Albert, MJ. The prevalence of antimicrobial resistance and carriage of virulence genes in Staphylococcus aureus isolated from food handlers in Kuwait City restaurants. (2009). BMC Res Notes; 2:108. http://dx.doi.org/10.1186/1756-0500-2-108.

      [17] Cotar, A-I., Chifiriuc, M-C., Dinu, S., Bucur, M., Iordache, C., Banu, O., Dracea, O., Larion, C, Lazar, V. Screening of molecular virulence markers in Staphylococcus aureus and Pseudomonas aeruginosa strains isolated from clinical infections. (2010). International journal of molecular sciences; 11:5273-5291.

      [18] Shi, D., Higuchi, W., Takano, T., Saito, K., Ozaki, K., Takano, M., Nitahara, Y, Yamamoto, T. Bullous Impetigo in Children with Methicillin-Resistant Staphylococcus aureus Alone or in Combination with Methicillin-Susceptible S. aureus–Genetic Characteristics, Including Assessment of Exfoliative Toxin Gene Carriage. (2011). J Clin Microbiol.

      [19] Xie, Y., He, Y., Gehring, A., Hu, Y., Li, Q., Tu, S-I, Shi, X. Genotypes and toxin gene profiles of Staphylococcus aureus clinical isolatesfrom China. (2011). PLoS One; 6:e28276. http://dx.doi.org/10.1371/journal.pone.0028276.

      [20] RůžiÄková, V., Voller, J., PantůÄek, R., Petráš, P, DoÅ¡kaÅ™, J. Multiplex PCR for detection of three exfoliative toxin serotype genes inStaphylococcus aureus. (2005). Folia Microbiol (Praha); 50:499-502. http://dx.doi.org/10.1007/BF02931437.

      [21] Deurenberg, RH, Nieuwenhuis, RF., Driessen, C., London, N., Stassen, FR., Tiel, FH, Stobberingh, EE, Vink, C. The prevalence of the Staphylococcus aureus tst gene among communityâ€and hospitalâ€acquired strains and isolates from Wegener's Granulomatosis patients. (2005). FEMS Microbiol Lett; 245:185-189. http://dx.doi.org/10.1016/j.femsle.2005.03.002.

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  • How to Cite

    Ramazanzadeh, R., Mohammadi Talvar, H., Mirzaii, M., Hasheminasab, S. S., & Narenji, H. (2015). Investigating prevalence of pathogenic genes (ETA and TSST-1) in Staphylococcus aureus isolated from different wards of the hospitals by PCR method. International Journal of Scientific World, 3(2), 239-243. https://doi.org/10.14419/ijsw.v3i2.5168

    Received date: 2015-08-09

    Accepted date: 2015-08-31

    Published date: 2015-09-05