Phytochemical analysis and proximate composition of Vernonia amygdalina

  • Authors

    • Usunomena Usunobun benson idahosa university, benin city, edo state, nigeria
    • Okolie Ngozi University of Benin
    2016-03-08
    https://doi.org/10.14419/ijsw.v4i1.5845
  • Vernonia amygdalina, Phytochemicals, Proximate, Ethanol.

  • Abstract

    Background: Vernonia amygdalina is used to prepare dishes as well as a multi-purpose fodder tree with high biomass yield. This study was conducted to determine phytochemicals and proximate composition of Vernonia amygdalina leaves.

    Methods: Evaluation of Vernonia amygdalina for phytochemicals and proximate composition were conducted using standard methods.

    Results: Result showed that Vernonia amygdalina contain phytochemicals such as alkaloids, tannins, flavonoids, saponins, triterpenoids, steroids, cardiac glycosides, and reducing sugar. Results obtained on the proximate composition shows that Vernonia amygdalina in percentage (%) contain dry matter (90.68 ± 0.77), crude protein (22.81 ± 0.17), crude fiber (18.17±0.06), moisture (9.32 ± 0.67), ash (16.65 ± 0.09), crude fat (4.34 ± 0.04) and carbohydrate (38.03 ± 0.06).

    Conclusion: The presence of phytochemicals like saponins, tannins, alkaloids and flavonoids explains the medicinal potentials of Vernonia amygdalina leaves in therapeutic uses. Also the leafy vegetables if consume in sufficient amount would contribute greatly to the nutritional requirement for human health and to the food security of Nigerian population.

  • References

    1. [1] Igile GO, Oleszek W, Jurzysta M, Burda S, Fafunso M, & Fasanmade AA. (1995). Nutritional assesment of Vernonia amygdalina leaves in growing mice. Journal of Agriculture and Food Chemistry43, 2162-2166.http://dx.doi.org/10.1021/jf00056a038.

      [2] Bosch CH, Borus DJ & Siemonsma JS. (2005). Vegetables of Tropical Africa. Conclusions and Recommendations Based on PROTA 2: ‘Vegetables’. PROTA Foundation, Wageningen, Netherlands. 10 modules, pp68.

      [3] Mayhew S & Penny A. (1988). Macmillan Tropical and Subtropical Foods. Macmillan Publishers, London, p107.

      [4] Gill LS (1992). Ethnobotanical uses of plants in Nigerial. University of Binin press, Benin City, 350.

      [5] Argheore EM, Makkar HPS & Becker K. (1998). Feed value of some browse plants from the central zone of Delta State Nigeria. Tropical Science38(2), 97–104.

      [6] Abosi AO &Raseroka BH. (2003). In vivo antimalarial activity of Vernonia amygdalina. British Journal of Biomedical Science 60(2), 89- 91.

      [7] Izevbige EB, Bryant TL, & Walker AA (2004). novel natural hibitor of extracellular signal regulated kinases and human breast cancer cell growth. Experimental Biology andMedicine 229(2), 163-169.

      [8] Akah PA & Okafor CI (1992).Hypoglycemic effect of Vernonia amygdalina in experimental rabbits Plant Medicine andResearch1, 6–10.

      [9] Nwanjo HU (2005). Efficacy of aqueous leaf extract of Vernonia amygdalina on plasma lipoprotein and oxidative status in diabetic ratmodels. Nigerian Journal of Physiological Science 20, 39-42.

      [10] Ebong PE, Atangwho IJ, Eyong EU, &Egbung GE. (2008). the antidiabetic efficacy of combined extracts from two continental plants: Azadirachta indica(A. Juss) (Neem) and Vernonia amygdalina (Del.) (African bitter leaf). American Journal of Biochemistry and Biotechnology 4, 239-244.http://dx.doi.org/10.3844/ajbbsp.2008.239.244.

      [11] AOAC (Association of Official Analytical Chemists). (2000). Official method of analysis. 15th Edition, Washington D.C. p212.

      [12] Sofowora LA. (1993). Medicinal plants and Traditional Medicine in Africa. Spectrum Books Ltd, Ibadan, pp55-71.

      [13] Trease GE & Evans WC. (1985). Pharmacognosy 11th Ed., Tindall Ltd, London, pp60-75.

      [14] Ayoola, GA, Coker HAB, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC &Atangbayila TO. (2008).Phytochemical screening and antioxidant activities of some selected medicinal plantsused for malarial therapy in South-Western Nigeria. Tropical Journal ofPharmaceutical Research 7, 1019-1024.

      [15] Ugwu OPC, Nwodo OFC, Joshua PE, Bawa A, Ossai EC &Odo CE.(2013). Phytochemical and Acute Toxicity Studies of MoringaoleiferaEthanol Leaf Extract. International Journal of Life SciencesBiotechnology and Pharma Research 2(2), 66-71.

      [16] Aiyegoro OA &Okoh AI. (2009). Phytochemical screening and polyphenolic antioxidant activity of aqueous crude leaf extract of Helichrysumpedunculatum. International Journal of Molecular Science 10, 4990–5001.http://dx.doi.org/10.3390/ijms10114990.

      [17] Omoregie ES,Osagie AU & Iruolaje EO (2011). In Vitro Antioxidant Activity And The Effect Of Methanolic Extracts Of Some Local Plants On Nutritionally Stressed Rats. Pharmacologyonline1, 23-56.

      [18] Haslam E. (1996). Natural Polyphenols (vegetable tannins) as drugs: possible modes of action. Journal of National Productivity 59, 205- 215.http://dx.doi.org/10.1021/np960040+.

      [19] Okwu DE. & Emenike IN. (2006). Evaluation of the phytonutrients and vitamin contents of Citrusfruits. International Journal ofMolecular Medicine and Advance Sciience 2, 1–6.

      [20] Adegboye MF, Akinpelu DA. &Okoh, A. (2008). The bioactive and phytochemical properties of Garcinia kola (Heckel) seed extract on some pathogens. African Journal of Biotechnology 7(21), 3934-3938.

      [21] Harbone, JB. (1998). Methods of extraction and isolation. In: Phytochemical Methods. Chapman and Hall, London;Pp60-66.

      [22] Francis C, George G, Zohar K, Harinder PS, Makhar LM, & Klaus B. (2002). The biological action of saponins in animal system: a review. British Journal of Nutrition88(6),587-605l.http://dx.doi.org/10.1079/BJN2002725.

      [23] Okwu DE.(2001).Evaluation of the chemical composition of medicinal plants belonging to Euphorbiaceae. Pakistan Veterinary Journal 14, 160-162.

      [24] Aiyegoro OA. &Okoh AI. (2010). Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifoliumDC. BMC Complementary and Alternative Medicine 10, 21-32.http://dx.doi.org/10.1186/1472-6882-10-21.

      [25] Yukari I, Youichi F, Ikuko N, &Itsuru Y. (1995). Quantitative HPLC analysis of cardiac glycosides in Digitalis purpurealeaves. JournalNational Productivity58(60), 897-901.

      [26] Yeap SK, Ho WY, Beh BK, Liang WS, Ky H, Yousr AHN &Alitheen, NB. (2010). Vernonia amygdalina, an ethnoveterinary and ethnomedical used green vegetable with multiple bio- activities. Journal of Medicinal Plants Research 4(25), 2787-2812.

      [27] Hassan LG & Umar KJ (2006).Nutritional value of Balsam Apple (Momordica balsaminaL.) leaves. Pakistan Journal Nutrition 5(6), 522-529.http://dx.doi.org/10.3923/pjn.2006.522.529.

      [28] Isong EU. AndIdiong. (1997). Comparative studies on the nutritional and toxic composition of three varieties of Leianthera Africana, Plants.Food and Human Nutrition51, 79-84.http://dx.doi.org/10.1023/A:1007922308985.

      [29] Ogle BM & Grivetti LE (1985).Legacy of the chameleon: Edible wild plants in the Kingdom of Swaziland, Southern Africa. A cultural, ecological nutritional study. Part IV: Nutritional analysis and conclusion, Ecology and Food. Nutrition17, 41-64.http://dx.doi.org/10.1080/03670244.1985.9990881.

      [30] Akindahunsi AA & Salawu.SO. (2005). Photochemical screening and nutrient-anti-nutrient composition of selected tropical greenvegetables, African Journal of Biotechnology4, 497-501

      [31] Antia BS, Akpan EJ, Okon PA &Umoren IU.(2006). Nutritive and Anti-Nutritive Evaluation of Sweet Potatoes (Ipomoea batatas) Leaves. Pakistan Journal of Nutrition5,166-168http://dx.doi.org/10.3923/pjn.2006.166.168.

      [32] Etuk EU, Bassey MN, Umoh UO & Inyang EG. (1998). Comparative nutritional studies on three local varieties of Heinsiacrinita, Plant Varieties Seeds11, 151-158.

      [33] Bailey R (2008), the Role of Proteins in the Body. About.com Guide to Biology.

      [34] Mensah JK, Okoli RI, Ohaju-Obodo JO and Eifediyi K. (2008).Phytochemical, nutritional and medical properties of some leafy vegetables consumed by Edo people of Nigeria. African Journal of Biotechnology7, 2304-2309.

      [35] Henry S. (2004). Fiber: An All Natural “Medicine†for Type 2 Diabetes? Consumer Health Interactive.http://www.ahealthyme.com

      [36] Ylonen K, Saloranta C, Kronberg C, Leif G, Antti A, Suvi M, &Virtanean M. (2003). Associations of Dietary Fiber with Glucose Metabolism in Non-diabetic relatives of subjects with Type 2 Diabetes. Diabetes care 26, 1979-1985.http://dx.doi.org/10.2337/diacare.26.7.1979.

      [37] Lockeett CT, CalvertCC and Grivetti LE (2005). Energy and micronutrient Composition of dietary and Medicinal wild plants Consumed during drought: Study of Rural Fulani, Northeastern Nigeria. International Journal ofFood. Science and Nutrition51, 195-208.

      [38] Asibey-BerkoE,&Tayie FAK. (1999). Proximate Analysis of some under -utilized Ghanian vegetables. Ghana Journal ofScience 39, 8-16.

      [39] Faruq UZ, Sani A and Hassan LG (2002). Proximate composition of sickle pod. (Senna obtusfolia) leaves,Nigerian Journal of Basic andApplied Sciences11, 157-164.

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  • How to Cite

    Usunobun, U., & Ngozi, O. (2016). Phytochemical analysis and proximate composition of Vernonia amygdalina. International Journal of Scientific World, 4(1), 11-14. https://doi.org/10.14419/ijsw.v4i1.5845

    Received date: 2016-02-08

    Accepted date: 2016-03-01

    Published date: 2016-03-08