Molecular epidemiology of staphylococcus aureus isolated from the patients, personnel and hospital environments in Sanandaj (Iran)

  • Authors

    • Hadi Mohammadi Talvar Islamic Azad University, Science and Research Campus, Kurdistan branch, Sanandaj, Iran.
    • Rashid Ramazanzadeh Cellular & Molecular Research Center and Microbiology Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj- Iran
    • Mehdi Mirzaii Faculty of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
    • Seyed Sajjad Hasheminasab Veterinary parasitology, Damdaran Taavoni, Molavi st, Dehgolan, Sanandaj-Iran
    2017-02-01
    https://doi.org/10.14419/ijsw.v5i1.7154
  • Methicillin-Resistant Staphylococcus Aureus (MRSA), Meca, Spa Typing.
  • Abstract

    The aim of this study was to determine the prevalence and characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates from the patients, personnel and environments of a hospital in Sanandaj. During this study, 30 MRSA isolates were collected and analyzed with using spa typing method. Out of the 30 S. aureus isolates, 28 (93.33%) was methicillin resistant (MRSA),16spastypesp(t030,t711,t002,t1556,5t325,t267,t1358,t230,t2422,t969,t1814,t037,t300,t044,t180,andat5598)9wereefounduin differentewardsrof thethospital.aTheTmostofrequentewereet0300(17.27%),%t7117(13.73%).%OUrOdataademonstratedtthaththetoverall, resistancescto erythromycin, igentamycin, iandaciprofloxacin, iwereemostofrequente (87.5,.81.25,2anda62.52respectively).y).

  • References

    1. [1] Shopsin, B. and B.N. Kreiswirth, Molecular epidemiology of methicillin-resistant Staphylococcus aureus. Emerging infectious diseases, 2001. 7(2): p. 323. https://doi.org/10.3201/eid0702.010236.

      [2] Church, D.L., et al., Comparison of automated repetitive-sequence–based polymerase chain reaction and< i> spa</i> typing versus pulsed-field gel electrophoresis for molecular typing of methicillin-resistant< i> Staphylococcus aureus</i>. Diagnostic microbiology and infectious disease, 2011. 69(1): p. 30-37. https://doi.org/10.1016/j.diagmicrobio.2010.09.010.

      [3] Deurenberg, R.H. and E.E. Stobberingh, The evolution of< i> Staphylococcus aureus</i>. Infection, genetics and evolution, 2008. 8(6): p. 747-763. https://doi.org/10.1016/j.meegid.2008.07.007.

      [4] Vainio, A., Molecular methods for the epidemiological analysis of methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus pneumoniae. 2012.

      [5] Hallin, M., A.W. Friedrich, and M.J. Struelens, spa typing for epidemiological surveillance of Staphylococcus aureus, in Molecular Epidemiology of Microorganisms. 2009, Springer. p. 189-202.

      [6] Struelens, M., et al., Laboratory tools and strategies for methicillinâ€resistant Staphylococcus aureus screening, surveillance and typing: state of the art and unmet needs. Clinical microbiology and infection, 2009. 15(2): p. 112-119. https://doi.org/10.1111/j.1469-0691.2009.02698.x.

      [7] Frenay, H., et al., Molecular typing of methicillin-resistantStaphylococcus aureus on the basis of protein A gene polymorphism. European Journal of Clinical Microbiology and Infectious Diseases, 1996. 15(1): p. 60-64. https://doi.org/10.1007/BF01586186.

      [8] Frenay, H., et al., Discrimination of epidemic and nonepidemic methicillin-resistant Staphylococcus aureus strains on the basis of protein A gene polymorphism. Journal of clinical microbiology, 1994. 32(3): p. 846-847.

      [9] Friedrich, A., et al., SeqNet. org: a European laboratory network for sequence-based typing of microbial pathogens. Euro surveillance: bulletin europeen sur les maladies transmissibles= European communicable disease bulletin, 2005. 11(1): p. E060112. 4-E060112. 4.

      [10] Stefani, S., et al., Meticillin-resistant< i> Staphylococcus aureus</i> (MRSA): global epidemiology and harmonisation of typing methods. International journal of antimicrobial agents, 2012. 39(4): p. 273-282. https://doi.org/10.1016/j.ijantimicag.2011.09.030.

      [11] Mahon, C., G. Manuselis, and D. Lehman, Textbook of Diagnostic Microbiology, Saunders. 2000, ISBN 0-7216-7917-X.

      [12] Wang, S.-C., et al., Distribution of superantigenic toxin genes in< i> Staphylococcus aureus</i> isolates from milk samples of bovine subclinical mastitis cases in two major diary production regions of China. Veterinary microbiology, 2009. 137(3): p. 276-281. https://doi.org/10.1016/j.vetmic.2009.01.007.

      [13] Maniatis, T., E.F. Fritsch, and J. Sambrook, Molecular cloning: a laboratory manual. Vol. 545. 1982: Cold Spring Harbor Laboratory Cold Spring Harbor, NY.

      [14] Cockerill, F.R., Performance standards for antimicrobial susceptibility testing: twenty-first informational supplement. 2011: Clinical and Laboratory Standards Institute (CLSI).

      [15] Stegger, M., et al., Rapid detection, differentiation and typing of methicillinâ€resistant Staphylococcus aureus harbouring either mecA or the new mecA homologue mecALGA251. Clinical Microbiology and Infection, 2012. 18(4): p. 395-400. https://doi.org/10.1111/j.1469-0691.2011.03715.x.

      [16] Fatholahzadeh, B., et al., Characterisation of genes encoding aminoglycoside-modifying enzymes among meticillin-resistant< i> Staphylococcus aureus</i> isolated from two hospitals in Tehran, Iran. International journal of antimicrobial agents, 2009. 33(3): p. 264-265. https://doi.org/10.1016/j.ijantimicag.2008.09.018.

      [17] Song, J.-H., et al., Spread of methicillin-resistant Staphylococcus aureus between the community and the hospitals in Asian countries: an ANSORP study. Journal of antimicrobial chemotherapy, 2011. 66(5): p. 1061-1069. https://doi.org/10.1093/jac/dkr024.

      [18] Borg, M., L. Camilleri, and B. Waisfisz, Understanding the epidemiology of MRSA in Europe: do we need to think outside the box? Journal of Hospital Infection, 2012. 81(4): p. 251-256. https://doi.org/10.1016/j.jhin.2012.05.001.

      [19] Vahdani, P., et al., Antibiotic resistant patterns in MRSA isolates from patients admitted in ICU and infectious ward. Tanaffos, 2004. 3(11): p. 37-44.

      [20] Alp, E., et al., MRSA genotypes in Turkey: persistence over 10 years of a single clone of ST239. Journal of Infection, 2009. 58(6): p. 433-438. https://doi.org/10.1016/j.jinf.2009.04.006.

      [21] Mirzaii, M., et al., Molecular investigation of< i> Staphylococcus aureus</i> isolated from the patients, personnel, air and environment of an ICU in a hospital in Tehran. Journal of infection and public health, 2014.

      [22] He, W., et al., Population structure and characterisation of< i> Staphylococcus aureus</i> from bacteraemia at multiple hospitals in China: association between antimicrobial resistance, toxin genes and genotypes. International journal of antimicrobial agents, 2013. 42(3): p. 211-219. https://doi.org/10.1016/j.ijantimicag.2013.04.031.

      [23] Xiao, Y.-H., et al., Epidemiology and characteristics of antimicrobial resistance in China. Drug resistance updates, 2011. 14(4): p. 236-250. https://doi.org/10.1016/j.drup.2011.07.001.

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  • How to Cite

    Mohammadi Talvar, H., Ramazanzadeh, R., Mirzaii, M., & Hasheminasab, S. S. (2017). Molecular epidemiology of staphylococcus aureus isolated from the patients, personnel and hospital environments in Sanandaj (Iran). International Journal of Scientific World, 5(1), 54-57. https://doi.org/10.14419/ijsw.v5i1.7154

    Received date: 2016-12-26

    Accepted date: 2017-01-23

    Published date: 2017-02-01