Comparative study of antibiotic resistance in bacteria isolated from dog and chicken

 
 
 
  • Abstract
  • Keywords
  • References
  • PDF
  • Abstract


    The emergence of antibiotic resistance has caused a threat to both human and animal population. This research was designed to investigate and compare the antibiotic resistance of bacteria isolated from chicken and dogs. A hundred and twelve samples of freshly dead chicken and eighty nine blood samples of sick dogs were analyzed. Pure culture of isolates were identified using cultural, morphological and biochemical characteristic. In vitro, susceptibility of the identified isolates against antimicrobial agents were determined by the standard disk diffusion procedure. One hundred and six isolates were recovered from chicken while 27 isolates were recovered from dogs. The organisms isolated include E. coli, Haemophilus sp, Pasturella sp, Klebsiella sp, Enterobacter sp, Salmonella sp, Staphylococcus sp., Micrococcus sp., Pseudomonas sp, Proteus sp, and Listeria sp. The antibiotic resistance showed that, gram-negative bacteria showed more resistance to the antibiotics used in this research compare to the gram-positive bacteria. This trend was found in isolates from both dog and chicken. In like manner, the bacteria isolates recovered from chicken showed a greater resistance when compare with the bacteria isolates recovered from dog. The increased resistance found in poultry makes poultry a suspect of residual resistance gene and probably reservoir for transmission.


  • Keywords


    : Antibiotics; Dog, Feed; Poultry.

  • References


      [1] Arora, A. K., Virmani, S. K. J. and Oberoi, M. S. (2005). Isolation, characterization and antibiogram of Pasteurella multocida isolates from different animal species. Indian J. Anim. Sci., 75: 749-752.

      [2] Atere, A. V. (2016). Multidrug resistant Salmonella sp isolated from chicken. International Journal of Biological research, 4 (1) 64-66.

      [3] Atere, A. V., Bamikole, A. M., Oluyege A. O., Ajurojo, O. A. and Alo, O. S. (2016). Prevalence and antibiotic resistance of Pasteurella multocida isolated from chicken in Ado-Ekiti metropolis. International journal of scientific world 4 (2): 40-42.

      [4] Atere, V. A, Bamikole, A. M. and Ajurojo, O. A. (2015b) Antibiotic Susceptibility of Bacteria Isolated from Poultry Feeds Sold in Ado Ekiti, Nigeria. Journal of Advancement in Medical and Life Sciences V3I2. DOI: 10.15297/JALS.V3I2.06

      [5] Atere, V. A., Bamikole, A. M., Ajurojo, O. A. and Alo, O. S. (2015a). Antimicrobial Resistance Pattern of Pathogenic Escherichia coli Isolated from Chicken Liver and Trachea. Journal of Advancement in Medical and Life Sciences. V3I3.

      [6] Dashe, Y. D., Raji, M. A., Abdu, P. A., Oladele, B. S. and Sugun, M. Y. (2013). Multidrug Resistant Pasteurella multocida Strains Isolated from Chickens with Cases of Fowl Cholera in Jos, Nigeria. Int. J. Poult. Sci., 12 (10): 596-600. https://doi.org/10.3923/ijps.2013.596.600.

      [7] Everlon, C. R., Patrick, J. B., Renato, P. M. and Fernando, A. Á. (2013) Identification and antimicrobial susceptibility patterns of Pasteurella multocida isolated from chickens and Japanese quails in Brazil. Brazilian Journal of Microbiology 44 (1): 161-164. https://doi.org/10.1590/S1517-83822013000100023.

      [8] Geornaras, I., Hastings, J. W. and Holy, A. (2001). Genotypic analysis of Escherichia coli strains from poultry carcasses and their susceptibilities to antimicrobial agents. Appl. Environ. Microbiol, 67: 1940-1944, https://doi.org/10.1128/AEM.67.4.1940-1944.2001.

      [9] Joint Expert Advisory Committee on Antibiotic Resistance (JETACAR) (1999). Report on The Use of Antibiotics in Food-producing Animals: Antibiotic resistant Bacteria in Animals and Humans, Commonwealth of Australia.

      [10] Rosa, C. A. R., Ribeiro, J. M. M., Fraga, M. J., Gatti, M. J., Cavaglieri, L. R., Magnoli, C. E., Dalcero, A. M. and Lopes, C. W. G. (2006). Mycoflora of poultry feeds and ochratoxin-producing ability of isolated Aspergillus and Penicillium species. Veterinary Microbiology, 113: 89-96. https://doi.org/10.1016/j.vetmic.2005.10.031.

      [11] San Millan, A., Escudero, J. A., Gutierrez, B., Hidalgo, L., Garcia, N., Montserrat, L., Dominguez, L., Zorn, G. B. (2009). Multi-resistance in Pasteurella multocida is mediated by coexistence of small plasmids. Antimicrob Agents Chemother 53:3399-3404. https://doi.org/10.1128/AAC.01522-08.

      [12] Uwaezuoke, J. C. and Ogbulie, J. N. (2008) Microbiological quality of commercially available poultry feeds sold in parts of Eastern Nigeria. J. Appl. Sci. Environ. Manage. 12(1) 113 – 117.

      [13] Van-den Bogaard, A. E., London, N. and Stobberingh, E. E. (2000). Antimicrobial resistance in pig faecal samples from The Nether-lands (five abattoirs) and Sweden. J. Antimicrob. Chemother. 45: 663-671. https://doi.org/10.1093/jac/45.5.663.

      [14] Van-den Bogaard, A. E., London, N., Driessen, C. and Stobberingh, E. E. (2001). Antibiotic resistance of faecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. J. Antimicrob. Chemother. 47:763–771. https://doi.org/10.1093/jac/47.6.763.

      [15] White, D. G., Zhao, S., Simjee, S., Wagner, D. D. and McDermott, P .F. (2002). Antimicrobial resistance of foodborne pathogens. Mi-crobial. Infect. 4: 405-412.


 

View

Download

Article ID: 8744
 
DOI: 10.14419/ijsw.v6i1.8744




Copyright © 2012-2015 Science Publishing Corporation Inc. All rights reserved.