Effects of estrogen benzoate on the microanatomy of adrenal gland in female Albino rats

Abstract

The present study was conducted in the department of Anatomy Government Medical College Srinagar to observe the effect of graded doses of estrogen benzoate on the microanatomy of adrenal gland in female Albino rats by giving variable doses of estrogen to these animals for variable periods. Sixty female albino rats weighing on an average 100gms were used for this study. The animals taken from animal house of Government Medical College Srinagar were divided randomly into three groups. Group A served as control, Group B and C received daily low and high doses of estrogen benzoate diluted in coconut oil respectively. From each group animals were sacrificed at intervals of one, three, six and twelve weeks.5-6 micrometer thick histological sections of adrenal gland were cut, fixed on glass slides and stained with Haematoxyline & Eosin. Microscopic changes in the adrenal gland were recorded. It was observed that estrogen benzoate produces dose and duration dependent histopathological changes in the adrenal gland of female albino rats in the form of thickening of the glandular capsule, dilatation and congestion of sinusoids both in the cortex and medulla of the gland.