Mediterranien cuttlefish sepia officinalis squid ink is cytotoxic but does not inhibit Glioblastoma U87 tumor cells proliferation, with high nutritional values of edible viscera

  • Authors

    • Soufia Chabchoub Ellouz institut pasteur de Tunis
    • Hazem Benmabrouk Tunis El-Manar University: Laboratory of Venoms Biomolecules and Therapeutics, Institut Pasteur de Tunis Tunisia
    • olfa baroudi Tunis El-Manar University: Genetics, Immunology and Human Pathology Laboratory. Faculty of Science Tunis Tunisia
    • Naceur Ayed
    • Naziha Marrakchi Tunis El-Manar University: Laboratory of Venoms Biomolecules and Therapeutics, Institut Pasteur de Tunis Tunisia
  • This study aims to identify nutritional values of some viscera’s cuttlefish Sepia officinalis commonly edible in Tunisia (Nidamental gland, hepatopancreas, male gonad, squid ink) and to evaluate the role of cuttlefish ink, on viability and proliferation of human glioblastoma cells U87.Biochemical analysis shows that solid and liquid viscera are rich in water, in Protein and in total Ash. They contain less fat and Carbohydrates. Energy values are substantial. Sex and Cooking over low have no significant effect on these nutritional values. These results lead us to encourage the use of these marine co-products (generally rejected), not only for food but for prevent and reduce the incidence of certain diseases. In fact our results show also a cytotoxic effect of cuttlefish ink at increasing doses on human cells cancer Glioblastoma U87 for an incubation time of 5 hours with a concentration higher than 20?g/ml. The IC50 value is 25?g/ml which may be due to tyrosinase, responsible for the cephalopod ink toxic. Supernatant of cuttlefish ink Sepia offinalis does not block proliferation of cancer cells U87 during 4 days of incubation. Further analyses are warranted and necessary to substantiate and complete our findings such as the study of the effect of this ink in adhesion and migration of Glioblastoma cells U87.

    Keywords: Edible Viscera, Cuttlefish Sepia Officinalis, Supernatant Cuttlefish Ink, Nutritional Values, Viability, Proliferation, Human Glioblastoma U87.

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    Chabchoub Ellouz, S., Benmabrouk, H., baroudi, olfa, Ayed, N., & Marrakchi, N. (2014). Mediterranien cuttlefish sepia officinalis squid ink is cytotoxic but does not inhibit Glioblastoma U87 tumor cells proliferation, with high nutritional values of edible viscera. International Journal of Basic and Applied Sciences, 3(2), 146-154.