Ethanol extraction of orange peels from agricultural waste and its activity against four species of the bacteria

  • Authors

    • Osamah. A Tuqi Alkut- university college – Iraq wasit
    • Tabark abdukarim Hussein Alkut- university college – Iraq wasit
    • Mustafa Khudair Abbas Alkut hospital for Gynecology and Pediatrics – Iraq wasit
    • Mustafa Badr Ghazal Al Zahraa Teaching Hospital –Iraq wasit
    • Aya Aziz Hussein Alkut hospital for Gynecology and Pediatrics – Iraq wasit
    2023-12-20
    https://doi.org/10.14419/dkxkwf51

  • Abstract

    The present study was amid the Hot extraction of orange peels. It evaluated its ability to inhibit the bacteria growth against four types, i.e. Bacillus anthracis (B anthracis), Streptococcus pyogenes (S. pyogenes), Yersinia pestis (Y. pestis) and Klebsiella pneumonia (K. pneumonia). Hot-extraction method was used to extract orange peels (EPO) by using ethanol as a solvent. Five concentrations of the extract orange peels were treated against the bacteria. The results showed significant effects against all types of bacteria, where the results were 15mm against B. anthracis, while S. pyogenes showed more effect, reaching 17mm. Moreover, the measurements of the EPO zone against Y. pestis were 12mm, while its zone against K. pneumonia was 15mm. The obtained results can give clear evidence of the ability of EPO to be used as an antibacterial agent. The current study has achieved positive results by utilizing agricultural waste and converting it into useful materials in the biological field, as well as reducing environmental pollution.

     

  • References

    1. Jimenez-Lopez C, Fraga-Corral M, Carpena M, García-Oliveira P, Echave J, Pereira AG, Lourenço-Lopes C, Prieto MA, Simal-Gandara J. Agriculture waste valorisation as a source of antioxidant phenolic compounds within a circular and sustainable bioeconomy. Food & function. 2020;11(6):4853-77. https://doi.org/10.1039/D0FO00937G.
    2. Kainat S, Arshad MS, Khalid W, Zubair Khalid M, Koraqi H, Afzal MF, Noreen S, Aziz Z, Al-Farga A. Sustainable novel extraction of bioactive compounds from fruits and vegetables waste for functional foods: A review. International Journal of Food Properties. 2022 Dec 31;25(1):2457-76. https://doi.org/10.1080/10942912.2022.2144884.
    3. Wang Z, Mei X, Chen X, Rao S, Ju T, Li J, Yang Z. Extraction and recovery of bioactive soluble phenolic compounds from brocade orange (Citrus sinensis) peels: Effect of different extraction methods thereon. LWT. 2023 Jan 1; 173: 114337. https://doi.org/10.1016/j.lwt.2022.114337.
    4. Mudhafar M, Zainol I, Jaafar CN, Alsailawi HA, Majhool AA, Alsaady M. Phytochemical Screening and Characterization of Meliadubia Leaves Extract for Antimicrobial Activity against Escherichia coli and Staphylococcus aureus. Indian Journal of Ecology. 2020;47(2):493-6.
    5. Fayaz AM, Balaji K, Girilal M, Yadav R, Kalaichelvan PT, Venketesan R. Biogenic synthesis of silver nanoparticles and their synergis-tic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomedicine: Nanotechnology, Biology and Med-icine. 2010 Feb 1;6(1):103-9. https://doi.org/10.1016/j.nano.2009.04.006.
    6. Wang D, Lin Z, Ding X, Hu J, Liu Y. The Comparison of the Combined Toxicity between Gram‐negative and Gram‐positive Bacteria: a Case Study of Antibiotics and Quorum‐sensing Inhibitors. Molecular Informatics. 2016 Feb;35(2):54-61. https://doi.org/10.1002/minf.201500061.
    7. Ilizirov Y, Formanovsky A, Mikhura I, Paitan Y, Nakonechny F, Nisnevitch M. Effect of photodynamic antibacterial chemotherapy combined with antibiotics on Gram-positive and Gram-negative bacteria. Molecules. 2018 Nov 30;23(12):3152. https://doi.org/10.3390/molecules23123152.
    8. Maxson T, Kongphet-Tran T, Mongkolrattanothai T, Travis T, Hendricks K, Parker C, McLaughlin HP, Bugrysheva J, Ambrosio F, Michel P, Cherney B. Systematic review of in vitro antimicrobial susceptibility testing for Bacillus anthracis, 1947–2019. Clinical In-fectious Diseases. 2022 Oct 15;75(Supplement_3): S373-8. https://doi.org/10.1093/cid/ciac520.
    9. Athamna A, Athamna M, Abu-Rashed N, Medlej B, Bast DJ, Rubinstein E. Selection of Bacillus anthracis isolates resistant to antibiot-ics. Journal of Antimicrobial Chemotherapy. 2004 Aug 1;54(2):424-8. https://doi.org/10.1093/jac/dkh258.
    10. Apriliana UI, Ruhiat E, Wibawa H, Untari T, Indarjulianto S. Resistance finding of Bacillus anthracis towards penicillin in East Java, Central Java, and Yogyakarta Provinces, Indonesia. InIOP Conference Series: Earth and Environmental Science 2023 May 1 (Vol. 1174, No. 1, p. 012027). IOP Publishing. https://doi.org/10.1088/1755-1315/1174/1/012027.
    11. Lei C, Kumar S. Yersinia pestis antibiotic resistance: a systematic review. Osong Public Health and Research Perspectives. 2022 Feb;13(1):24. https://doi.org/10.24171/j.phrp.2021.0288.
    12. Xiao L, Qi Z, Song K, Lv R, Chen R, Zhao H, Wu H, Li C, Xin Y, Jin Y, Li X. Interplays of mutations in waaA, cmk, and ail contribute to phage resistance in Yersinia pestis. Frontiers in Cellular and Infection Microbiology. 2023 May 26; 13: 1174510. https://doi.org/10.3389/fcimb.2023.1174510.
    13. Alam N, Tahir K, Nazir S, Khan AU, Albalawi K, Refat MS, Almarhoon ZM, Jevtovic V, Al-Shehri HS, Aldawsari AM. Effect of light-dark conditions on inhibition of Gram positive and gram-negative bacteria and dye decomposition in the presence of photocatalyst Co/ZnO nanocomposite synthesized by ammonia evaporation method. Photodiagnosis and Photodynamic Therapy. 2022 Jun 1; 38: 102853. https://doi.org/10.1016/j.pdpdt.2022.102853.
    14. Gamelas SR, Vieira C, Bartolomeu M, Faustino MA, Tomé JP, Tomé AC, Almeida A, Lourenço LM. Photodynamic inactivation of pathogenic Gram-negative and Gram-positive bacteria mediated by Si (IV) phthalocyanines bearing axial ammonium units. Journal of Photochemistry and Photobiology B: Biology. 2022 Aug 1;233: 112502. https://doi.org/10.1016/j.jphotobiol.2022.112502.

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  • How to Cite

    Tuqi, O. A., Hussein, T. abdukarim, Abbas , M. K., Ghazal, M. B., & Hussein, A. A. (2023). Ethanol extraction of orange peels from agricultural waste and its activity against four species of the bacteria. International Journal of Basic and Applied Sciences, 12(1), 22-25. https://doi.org/10.14419/dkxkwf51