Allelopathic activities of selected Mucuna pruriens on the germination and initial growth of lettuce

  • Authors

    • Kwame Appiah 1 Department of International Environmental And Agricultural Science, Tokyo University Of Agriculture and Technology. 2 Department of Crop Science, University of Ghana.
    • Christiana Amoatey 2 Department of Crop Science, University of Ghana
    • Yoshiharu Fujii 1 Department of International Environmental And Agricultural Science,Tokyo University Of Agriculture and Technology
  • Allelopathy, Elongation, Exudates, Leachates, Inhibition.
  • The study was conducted to test allelopathic effects of some Mucuna pruriens on the germination and growth of lettuce. The effects of root exudates and leaf leachates of eight mucuna genotypes; Mucuna pruriens var. utilis cv. cinza, Mucuna pruriens var. utilis cv. preta, Mucuna pruriens var. utilis cv. hassjo, Mucuna pruriens var. utilis cv. fvb, Mucuna pruriens 82/507, Mucuna pruriens 82/508, Mucuna pruriens 82/116, and Mucuna pruriens GA/94/002 were tested on the germination, radicle, and hypocotyl elongations of lettuce (Lactuca sativa var. Great Lakes 366). The effects of leaf leachates and root exudates of mucuna were tested using sandwich and plant-box methods respectively with lettuce as the receptor plant. Roots exudates inhibition varied significantly among genotypes (94.3%-98.5%). Root exudates of Mucuna pruriens var. utilis cv. fvb had the maximum inhibition (98.5%). Lettuce radicle inhibition gradually decreased with distance from the mucuna plant in a plant-box and with high correlation (r=0.910-0.952; p<0.05) between lettuce radicle inhibition and distance from mucuna roots. The effect of 50 mg oven-dried mucuna leaves had significant inhibition on lettuce radicle elongation (12.0%-15.8% of untreated control). Leaf leachates had less effect on the hypocotyl elongation of lettuce (50.5%-72.0% of untreated control) at the same application of oven-dried leaves (50 mg/10 ml agar).

  • References

    1. [1] Akobundu, I. O. (1987). Weed Science in the Tropics; Principles and Practices. Ibadan, Nigeria: John Wiley and Sons Ltd. (pp. 1, 2, 25, 54).

      [2] Heap, I. (2014). The International Survey of Herbicide Resistant Weeds. Online. Internet. Available Accessed on Wednesday, April 22, 2015.

      [3] Osei-Bonsu, P., Buckles, D., Soza, F. R. and Asibuo, J. Y. (1996). Edible cover crops. ILEIA, Newsletter 30-31.

      [4] Lawson I. Y. D., Dzomeku I. K., Asempa R., and Benson S. (2006). Weed Control in Maize Mucuna and Canavalia as Intercrops in the Northern Guinea Savanna Zone of Ghana. Journal of Agronomy. 5, 621-625.

      [5] Udensi, E.U., Akobundu, I.O., Ayeni, A.O., and Chikoye, D. (1999). Management of cogon grass (lmperata cylindrica) using mucuna (Mucuna pruriens var. utilis) and herbicides. Weed Technology. 13, 201-208.

      [6] Chikoye, D. and Ekeleme, F. (2001). Characteristics of ten mucuna accessions and their effects on the dry matter of Imperata cylindrica (L.) Rauesch. Biological Agriculture and Horticulture. 18, 191-201.

      [7] Osei, K., Moss, R., Nafeo, A., Addico, R., Agyemang A., and Asante J. S. (2011). Managing nematode pests and improving yield of pineapple with Mucuna pruriens in Ghana. Journal of Entomology and Nematology. 3(1), 20-24.

      [8] Nyalemegbe, K. K., Agbozo, G. K., and Addo-Quaye, A. A. (2011). The effect of mucuna residue management on the growth and yield of maize. Agricultural Science Research Journal. 1(10), 259-263.

      [9] Nicol, J. M., Turner, S. J., Coyne D. L., den Nijs L., Hockland, S., and Maafi, Z. (2011). Current nematode threats to world Agriculture, in: Jones et al. (Eds.), Genomics and Molecular Genetics of Plant-Nematode Interactions) 21-43.

      [10] Zasada, I. A., Klassen W., Meyer S. L. F., Codallo, M., and Abdul-Baki, A. A. (2006). Mucuna (Mucuna pruriens) extracts: Impact on Meloidogyne incognita survival and on Lycopersicon esculentum and Lactuca sativa germination and growth. Pest Management Science. 62, 1122-1127.

      [11] Arim, O. J., Waceke J. W., Waudo S. W., and Kimenju, J. W. (2006). Effects of Canavalia ensiformis and Mucuna pruriens intercrops on Pratylenchus zeae damage and yield of maize in subsistence agriculture. Plant soil. 284, 243-251.

      [12] Rodriguez-Kabana, R., Pinochet, J., Robertson, D. G., and Wells, L. (1992). Crop Rotation Studies with Mucuna (Mucuna deeringiana) for the Management of Meloidogyne spp. Journal of Nematology. 24(4S), 662-668.

      [13] Weaver, D. B., Rodriguez-Kabana, R., and Carden, E. L. (1998). Mucuna and Bahiagrass as rotation crops for management of Meloidegyne spp. and Heterodera glycines in soybean. Journal of Nematology. 30(4), 563-568.

      [14] Fujii, Y., Hiradate, S. (2007). Allelopathy: New Concepts and Methodology. Enfield, USA. Science Publishers.

      [15] Callaway, R. M., and Ridenour, W. M. (2004). Novel Weapons: Invasive Success and the Evolution of Increased Competitive Ability. Frontiers in Ecology Environment. 2(8), 436-443.[0436:NWISAT]2.0.CO;2.

      [16] Duke, S. O. (2010). Allelopathy: Current status of research and future of the discipline: A commentary. Allelopathy Journal. 2(1), 17-30.

      [17] Vyvyan, J. R. (2002). Allelochemicals as leads for new herbicides and agrochemicals. Tetrahedron. 58, 1631-1646.

      [18] Fujii, Y. (2003). Allelopathy in the natural and agricultural ecosystems and isolation of potent allelochemicals from mucuna (Mucuna pruriens) and hairy vetch (Vicia villosa). Biological Sciences in Space. 17(1), 6-13.

      [19] Fujii, Y., Shibuya T., and Yasuda T. (1991). L-3, 4-Dihydroxyphenylalanine as an allelochemical candidate from Mucuna pruriens (L) DC. Var. utilis. Agricultural and Biological Chemistry. 55(2), 617-618.

      [20] Fujii, Y., Shibuya T., and Yasuda T. (1992). Allelopathy of mucuna: Its discrimination and identification of L-DOPA as a candidate of allelopathic substance. Japan Agricultural Research Quarterly. 25, 238-247.

      [21] Fujii, Y. (1994). Screening of Allelopathic candidates by new specific discrimination and assessment methods for allelopathy, and the inhibition of L-DOPA as the allelopathic substance from the most promising mucuna (Mucuna pruriens). Bulletin of National Institute of Agro-Environmental Sciences. 10, 115–218 (in Japanese with English summary).

      [22] Fujii, Y., Shibuya, T., Nakata, K., Itani, T., Hiradate, S. and Parvez, M. M. (2004). Assessment Method for the Allelopathic Effect from Leaf Leachate. Weed Biology Management. 4, 19-23.

      [23] Shiraishi, S., Watanabe, I., Kuno, K., and Fujii, Y. (2002). Allelopathic activity of leaching from dry leaves and exudate from roots of ground cover plants assayed on agar. Weed Biology Management. 2, 133-142.

      [24] Nishihara, E., Parvez, M. M., Araya, H., Kawashima, S., and Fujii, Y. (2005). L-3-(3, 4-Dihydroxyphenyl)alanine (L-DOPA), an allelochemical exuded from mucuna (Mucuna pruriens) roots. Plant Growth Regulation. 45, 113-120.

      [25] Bhadoria, P. B. S. (2011). Allelopathy: A Natural Way towards Weed Management. American Journal of Experimental Agriculture. 1(1), 7-20.

      [26] Eucharia, O. N., and Edward O. A. (2010). Allelopathy as expressed by Mucuna pruriens and the possibility for weed management. International Journal of Plant Physiology and Biochemistry 2(1), 1-5.

      [27] Mushtaq, M. N., Sunohara, Y., and Matsumoto, H. (2013). Allelochemical L-DOPA induces quinoprotein adducts and inhibits NADH dehydrogenase activity and root growth of cucumber. Plant Physiology and Biochemistry. 70, 374-378.

      [28] Hachinohe, M., and Matsumoto, H. (2007). Involvement of melanin synthesis and reactive oxygen species in phytotoxic action of L-DOPA in carrot cells. Crop Protection. 26, 294-298.

  • Downloads

  • How to Cite

    Appiah, K., Amoatey, C., & Fujii, Y. (2015). Allelopathic activities of selected Mucuna pruriens on the germination and initial growth of lettuce. International Journal of Basic and Applied Sciences, 4(4), 475-481.