Efficiency of pseudomonas aeruginosa and Escherichia coli to treatment of effluent petroleum wastewater from AI-Nasiriya refinery – Iraq


  • Ihsan Flayyih Hasan AI-Jawhari Department of Environment and Pollution, Marshes Research Centre, Thi-Qar University
  • Noor Jabar Mhail Department of Chemistry , College of Science , Thi-Qar University
  • Saher Abed AI-RuthaAli Department of Chemistry , College of Science , Thi-Qar University






Bacteria, Refinery, Soil, Total Acidity, Wastewater.


Four bacteria isolated from the soil contaminated with petroleum hydrocarbon and wastewaters from the refinery   located in the south part of AI-Nasiriya city in Iraq,by using dilution method.The results showed that Pseudomonas aeruginosa was more dominant with 100% in soil and wastewaters in inside of refinery,and 93%,95% in soil and wastewaters in outside the refinery respectively.The results showed that the second dominant of bacteria was Esherchia coil with 92%,98% in soil and wastewaters in inside the refinery,and 83%,88% in soil and wastewaters in outside of refinery.The remaining two species of bacteria isolated with a very low frequency,these bacteria were Bacillus  subtilis and Klebsiella sp., B.subtilis was obtained with 10%,8% in soil and wastewaters in inside the refinery,and 10%,3% in soil and wastewaters in outside of refinery .In the same time that Klebsiella sp. Was obtained with 10% in soil and wastewaters in inside the refinery,and 8%,3% in soil and wastewaters in outside the refinery.The results showed that mixed two bacteria (high frequency)were more efficient to decrease Biological Oxygen Demande(BOD)with 59% in pH 4,0.5gm / I salt (NaCI) concentration.Chemical Oxygen Demande (COD) was decreased to 75%.The results showed that the Total Acidity(TA) in wastewater were increased to 82% mg / I in 40co ,pH 8 and 2.0 gm/ I NaCI , and Total Suspended Solid (TSS) was decreased to 50 mg / I in 40co, pH,2.0 gm / I NaCI . The results showed that the Total Petroleum Hydrocarbon (TPH) was removal with 99.99%, and in the same time the numbers of mixed bacteria were increased to 5x108 after incubation 48h in 40co .


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