4Hz mechanical vibration relieves pain through Na+/K+-ATPase α3 isoform-dependent brain tissue dehydration

  • Authors

    • Gohar Musheghyan Life Sciences International Postgraduate Educational Center, UNESCO Chair Armenia, Yerevan
    • Arevik Minasyan Life Sciences International Postgraduate Educational Center, UNESCO Chair Armenia, Yerevan
    • Gohar Arajyan Life Sciences International Postgraduate Educational Center, UNESCO Chair Armenia, Yerevan
    • Sinerik Ayrapetyan Life Sciences International Postgraduate Educational Center, UNESCO Chair Armenia, Yerevan
    2017-05-14
    https://doi.org/10.14419/ijbas.v6i2.7365
  • Brain, Cerebellum, Cortex, Mechanical Vibration, Tissue Hydration.

  • Abstract

    In this work the effect of 4Hz 30dB horizontal mechanical vibration (MV) on thermal pain threshold, hydration and [3H]-ouabain binding in brain and heart muscle tissues of rats was studied. It was revealed that 4Hz MV treatment for 10 minutes increased pain threshold, which was accompanied by brain and heart muscle tissue dehydration. In vitro state, hydration of brain and heart muscle tissues of sham animals was increased, while in 4Hz MV-treated animals the increase of brain tissue hydration was more pronounced and heart muscle tissues were dehydrated. The fact that 4Hz MV treatment also impacted heart muscle tissue hydration indicates that 4Hz MV effect on brain and heart muscle tissues is realized through a common messenger circulating in blood. The incubation of brain and heart muscle tissues in PS containing 10-4M and 10-9M ouabain led to tissue hydration in sham and 4Hz MV-treated animals. However, tissues of 4Hz MV-treated animals were less hydrated, and this hydration was accompanied by the decrease and increase of membrane receptors’ affinity at 10-4M and 10-9M ouabain concentrations, respectively. Based on the obtained data, it is suggested that pain-relieving effect of 4Hz MV is due to α3 isoform-dependent brain tissue dehydration.

  • References

    1. [1] M. Cardinale, MH. Pope, The effects of whole body vibration on humans: dangerous or advantageous? ActaPhysiol Hung. 90(3) 2003 195-206.https://doi.org/10.1556/APhysiol.90.2003.3.2.

      [2] M. Alves-Pereira, Infrasound and low frequency noise: Quantification in several rural and urban environments RevistaLusófona de Ciências e Tecnologias da Saude, 7(1) (2010) 91-108. http://recil.grupolusofona.pt/bitstream/handle/10437/2250/1237-4358-1-PB.pdf?sequence=1

      [3] V.A. Parsegian R.P. Rand, D.C. Rau, Osmotic stress, crowding, preferential hydration, and binding: A comparison of perspectives, Proc Nat Sci USA 97 (2000) 3987-3992.https://doi.org/10.1073/pnas.97.8.3987.

      [4] S.N. Ayrapetyan, M.A. Suleymanyan, A.A. Saghyan, S.S. Dadalyan, Autoregulation of electrogenic sodium pump, Cellular Molecular Neurobiology 4(4) (1984) 367-384.https://doi.org/10.1007/BF00733598.

      [5] S.N. Ayrapetyan, V.L. Arvanov, S.N. Maginyan et al., Further study of the correlation between Na-Pump activity and membrane chemosensitivity, Cell Mol Neurobiol 5(3) (1985) 231-243.https://doi.org/10.1007/BF00711009.

      [6] S.N. Ayrapetyan, G.Y. Rychkov, M.A. Suleymanyan, Effects of water flow on transmembrane ionic currents in neurons of Helix Pomatia and in squid giant axons, Comp Biochem Physiol 89A(2) (1988) 179-186. https://doi.org/10.1016/0300-9629(88)91076-6.

      [7] S. Ayrapetyan, N. Baghdasaryan, Y. Mikaelyan, et al., Cell bathing medium as a primary target and cell hydration as a universal biomarker for biological effects of non-ionizing radiation. Electromagnetic Fields in Biology and Medicine. USA: CRC Press, 2015; 193-216.

      [8] S. Ayrapetyan, The application of the theory of metabolic regulation to Pain, Pain Mechanism and Management. Amsterdam, Netherland: IOS Press, 1998; 3-14.

      [9] S. Ayrapetyan, G. Musheghyan, A. Deghoyan, The brain tissue dehydration as a mechanism of analgesic effect of hypertonic physiological solution in rats, Journal of International Dental and Medical Research 3(2) (2010) 93-98.

      [10] A. Heqimyan, A. Deghoyan, S. Ayrapetyan, Ketamine–induced cell dehydration as a mechanism of its analgesic and anesthetic effects, J Int Dent Med Res 4 (2011) 42-49.

      [11] A. Danielian, S. Ayrapetyan, Mechanism of anesthetic action of magnetic fields, Radiobiology & Radioecology 38(6) (1998) 908-912.

      [12] N.S. Baghdasaryan, Y.R. Mikayelyan, A.K. Nikoghosyan, S.N. Ayrapetyan, The impact of background radiation, illumination and temperature on EMF-induced changes of aqua medium properties, Electromagnetic Biology and Medicine 32(3) (2013) 390-400.https://doi.org/10.3109/15368378.2012.735206.

      [13] V. Martirosyan, N. Baghdasaryan, S. Ayrapetyan, Bidirectional frequency-dependent effect of extremely low-frequency electromagnetic field on E. coli K-12. Electromagnetic Biology and Medicine 32(3) (2013) 291-300. https://doi.org/10.3109/15368378.2012.712587.

      [14] K. Krnjevic, Cellular and synaptic actions of general anaesthetics, Gen Pharmacology 23 (1992) 965-75. https://doi.org/10.1016/0306-3623(92)90274-N.

      [15] R. Takahashi, M. Aprison, Acetylcholine content of discrete areas of the brain obtained by a near-freezing method, J Neurochem 11 (1964) 887-898.

      [16] S.N. Ayrapetyan, M.A. Sulejmanian, on the pump-induced cell volume changes, Comp Biochem. Physiol part A Comp Pharmacol 64(4) (1979) 571-575.

      [17] F. Lang, Mechanisms and significance of cell volume regulation, J Am Coll Nutr 26 (2007) 613-623.https://doi.org/10.1080/07315724.2007.10719667.

      [18] E.K. Hoffman, B.H. Sorensen, D.P. Sauter, I.H. Lambert, Role of volume-regulated and calcium-activated anion channels in cell volume homeostasis, Cancer and Drug resistance, Channels, Austin 9 (2015) 380-396. https://doi.org/10.1080/19336950.2015.1089007.

      [19] A. Deghoyan, A. Nikogosyan, A. Heqimyan, S. Ayrapetyan, Age-dependent effect of static magnetic field on brain tissue hydration, Electromagnetic Biology and Medicine 33(1) (2014) 58-67. https://doi.org/10.3109/15368378.2013.783852.

      [20] A.A. Sagian, S.N. Ayrapetyan, D.O. Carpenter, Low concentrations of ouabain stimulate Na:Ca exchange in neurons, Cell MolNeurobiol 16(4) (1996) 489-498.https://doi.org/10.1007/BF02150229.

      [21] P.F. Baker, M.P. Blaustein, A.L. Hodgkin, S.A. Steinhardt, The influence of calcium on sodium efflux in squid axons, J Physiol 200 (1969) 431-458. https://doi.org/10.1113/jphysiol.1969.sp008702.

      [22] A. Heqimyan, L. Narinyan, A. Nikoghosyan, S. Ayrapetyan, Age-dependent magnetic sensitivity of brain and heart muscles. Electromagnetic Fields in Biology and Medicine. USA: CRC Press, 2015; 217-230.

      [23] S.N. Ayrapetyan, Metabolically-dependent part of membrane potential and electrode properties of giant neuron membrane of mollusk, Biofizika 14(6) (1969) 1027-1031.

      [24] S.N. Ayrapetyan, on the physiological significance of pump-induced cell volume changes, Adv. Physiol. Sci. 23 (1980) 67-82.

      [25] M. Kojima, S. Ayrapetyan, K. Koketsu, On The Membrane Potential Independent Mechanism of Sodium Pump-Induced Inhibition of Spontaneous Electrical Activity of Japanese Land Snail Neurons, Comp. Biochem. Physiol. 77(A)(3) (1984) 577-583.

      [26] G.Y. Rychkov, M.A. Suleymanyan, S.N. Ayrapetyan, Dependence of water flow effect on the ionic currents of dialyzed neuron of somatic membrane fluidity, Biol Membrane 6(7) (1989) 733-739 (in Russian).

      [27] M.A. Suleymanian, V.Y. Ayrapetyan, V.B. Arakelyan, S.N. Ayrapetyan, The effect of osmotic gradient on the outward potassium current in dialyzed neurons of Helix Pomatia, Cell Mol Neurobiol 13(2) (1993) 183-190.https://doi.org/10.1007/BF00735374.

      [28] M.P. Blaustein, W.J. Lederer, Na+/Ca2+ exchange. Its physiological implications, Physiol Rev 79 (1999) 763-854.

      [29] Z. Xie, A. Askari, Na+/K+-ÐТPase as a signal transducer, Eur J Biochem 269 (2002) 2434-2439. https://doi.org/10.1046/j.1432-1033.2002.02910.x.

      [30] E. Dadasyan, G. Ayrapetyan, N. Baghdasaryan, Y. Mikayelyan, S Ayrapetyan, The Metabolic pathway of 4Hz mechanical vibration-induced effect on snail heart muscle contractility, Environmentalist 32(2) (2012) 166-174.https://doi.org/10.1007/s10669-011-9382-1.

      [31] J. Skou, The influence of some cations on an adenosine triphosphatase from peripheral nerves, Biochim Biophys Acta 23 (1957) 394-401.https://doi.org/10.1016/0006-3002(57)90343-8.

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  • How to Cite

    Musheghyan, G., Minasyan, A., Arajyan, G., & Ayrapetyan, S. (2017). 4Hz mechanical vibration relieves pain through Na+/K+-ATPase α3 isoform-dependent brain tissue dehydration. International Journal of Basic and Applied Sciences, 6(2), 29-35. https://doi.org/10.14419/ijbas.v6i2.7365

    Received date: 2017-02-15

    Accepted date: 2017-03-14

    Published date: 2017-05-14