Physical Properties and Total Phenolic Content by Different Drying and Extraction Techniques of Clinacanthus Nutans Leaves

  • Authors

    • Yvonne Melse Laurence
    • Mohammad Shaheen Khan
    • Mohd Sani Sarjadi
    2018-05-22
    https://doi.org/10.14419/ijet.v7i2.29.15141
  • Clinacanthus nutans, Drying, Extraction method, Physical properties, Phenolic content

  • Abstract

    This report presents a series of different drying condition and extraction methods on Clinacanthus nutans leaves. C. nutans leaves were dried using three different drying conditions: oven (40°C, 60°C and 80°C), vacuum oven and air drying. Then, the physical properties of dried leaves obtained were analyzed in terms of the moisture content and water activity. Thus, the series of extraction was performed using three different extraction techniques: soxhlet, maceration and ultrasound-assisted solvent extraction respectively. Finally, the total phenolic content of each extracts were determined with the Folin-Ciocalteu method. The results showed that the choice of drying method and extraction technique influenced the phenolic compound yield of C. nutans extract. Excellent performance of the combination of drying C. nutans leaves using laboratory oven at 60°C and soxhlet extraction obtained the highest total phenolic content yield at 65.92 ± 2.19 mg GAE/g.

     

     

  • References

    1. [1] Alam, A., Ferdosh, S., Ghafoor, K., Hakim, A., Juraimi, A. S., Khatib, A., & Sarker, Z. I. (2016). Clinacanthus nutans: A review of the medicinal uses, pharmacology and phytochemistry. Asian Pacific Journal of Tropical Medicine, 9(4), 402-409.

      [2] Arullappan, S., Rajamanickam, P., Thevar, N. & Kodimani, C.C. (2014). In Vitro Screening of Cytotoxic, Antimicrobial and Antioxidant Activities of Clinacanthus nutans (Acanthaceae) leaf extracts. Tropical Journal of Pharmaceutical Research, 13(9), 1455-1461.

      [3] Aslam, M. S., Ahmad, M. S., & Mamat, A. S. (2015). Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines. Evidence-based complementary and alternative medicine. International Journal of Pharmacy and Pharmaceutical Sciences, 7(2), 30-33.

      [4] Blainski, A., Lopes, G. C., & De Mello, J. C. P. (2013). Application and analysis of the Folin Ciocalteu method for the determination of the total phenolic content from Limonium Brasiliense L. Molecules, 18(6), 6852-6865.

      [5] Dai, J., & Mumper, R. J. (2010). Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10), 7313-7352.

      [6] Freeman, B. C., & Beattie, G. A. (2008). An overview of plant defenses against pathogens and herbivores. The Plant Health Instructor. DOI: 10.1094/PHI-I-2008-0226-01.

      [7] Ho, S. Y., Tiew, W. P., Priya, M., Mohamed, S. A. S., & Gabriel, A. A. (2013). Phytochemical analysis and antibacterial activity of methanolic extract of Clinacanthus nutans leaf. International Journal of Drug Development and Research, 5(3), 349-355.

      [8] Huang, D., Guo, W., Gao, J., Chen, J. & Olatunji, J. O. (2015). Clinacanthus nutans (Burm. f.) Lindau Ethanol Extract Inhibits Hepatoma in Mice through Upregulation of the Immune Response, Journal of Molecules. 20(9), 17405-17428.

      [9] Khoo, L. W., Mediani, A., Zolkeflee, N. K. Z., Leong, S. W., Ismail, I. S., Khatib, A., Shaari, K. & Abas, F. 2015. Phytochemical diversity of Clinacanthus nutans extracts and their bioactivity correlations elucidated by NMR based metabolomics. Phytochemistry Letters. 14,123-133.

      [10] Kumar, S. (2013). Efficient Method of Storage of Dry Herbs. Journal for Drugs and Medicines. 5(2), A1-A3.

      [11] Kunsorn, P., Ruangrungsi, N., Lipipun, V., Khanboon, A. & Rungsihirunrat, K. (2013). The identities and anti-herpes simplex virus activity of Clinacanthus nutans and Clinacanthus siamensis. Asian Pacific Journal of Tropical Biomedicine, 3(4): 284–290.

      [12] Lusia, B. M., Hasmadi, M., Zaleha, A. & Mohd Fadzelly, A. (2015). Effect of different drying methods on phytochemicals and antioxidant properties of unfermented and fermented teas from Sabah Snake Grass (Clinacanthus nutans Lind.) leaves. International Food Research Journal. 22(2), 661-670.

      [13] Mai, C. W., Yap, K.S. I., Kho, M. T., Ismail, N. H., Yusoff, K., Shaari, K., Chin, S.Y. & Lim, E. S. H. (2016). Mechanisms Underlying the Anti-Inflammatory Effects of Clinacanthus nutans Lindau Extracts: Inhibition of Cytokine Production and Toll-Like Receptor-4 Activation. Frontiers in Pharmacology. 7,7.

      [14] Pannangpetch, P. Laupattarakasem, P., Kukongviriyapan, V., Kukongviriyapan, U., Kongyingyoes, B. & Aromdee, C. (2007). Antioxidant activity and protective effect against oxidative hemolysis of Clinacanthus nutans (Burm.f) Lindau. Songklanakarin Journal of Science and Technology. 29(1), 1–9.

      [15] Peng, T. W., Wen, P. X., Han, C. J. & Akowuah, G. A. (2015). Effect of methanol extract of Clinacanthus nutans on serum biochemical parameters in rats. Journal of Molecules. 20,10822-10838

      [16] Pham, H. N. G. Van, Nguyen, V. T., Vuong, Q. V., Bowyer, M. C. & Scarlett, C. J. (2015). Effect of extraction solvents and drying methods on the physicochemical and antioxidant properties of Helicteres hirsuta Lour. Leaves. Journal of Technologies. 3,285-301

      [17] Puranik, V., Chauhan, D. K., Mishra, V., & Rai, G. K. (2012). Effect of drying techniques on the physicochemical and bioactive components of selected medicinal herbs. Ann Phytomed, 1, 23-29.

      [18] Raya, K. B., Ahmad, S. H., Farhana, S. F., Mohammad, M., Tajidin, N. E., & Parvez, A. (2015). Changes in phytochemical contents in different parts of Clinacanthus nutans (Burm. f.) lindau due to storage duration. Bragantia, 74(4), 445-452.

      [19] Sakdarat, S., Shuyprom, A., Pientong , C., Ekalaksananan, T. & Thongchai, S. (2009). Bioactive constituents from the leaves of Clinacanthus nutans Lindau. Bioorganic & Medicinal Chemistry. 17:1857–1860.

      [20] Solibun, A., & Sivakumar, K. (2016). Sabah snake grass extract pre-processing: Preliminary studies in drying and fermentation. In IOP Conference Series: Earth and Environmental Science (Vol. 36, No. 1, p. 012066). IOP Publishing.

      [21] Susantikarn, P., & Donlao, N. (2016). Optimization of green tea extracts spray drying as affected by temperature and maltodextrin content. Int. Food Res. J, 23, 1327-1331.

      [22] Thawaranantha, D., Balachandra, K., Jongtrakulsiri, S., Chavalittumrong, P., Bhumiswasdi, J. & Jayavasu, C. (1992). In vitro antiviral activity of Clinacanthus nutans on varicella zoster virus. Siriraj Hospital gazette. 44:285–291.

      [23] Tu, S. F., Liu, R. H., Cheng, Y. B., Hsu, Y. M., Du, Y. C., El-Shazly, M., ... & Chang, F. R. (2014). Chemical constituents and bioactivities of Clinacanthus nutans aerial parts. Molecules, 19(12), 20382-20390.

      [24] Wang, L., & Weller, C. L. (2006). Recent advances in extraction of nutraceuticals from plants. Trends in Food Science & Technology, 17(6), 300-312.

      [25] Zulkipli, I. N., Rajabalaya, R., Idris, A., Sulaiman, N. A., & David, S. R. (2017). Clinacanthus nutans: a review on ethnomedicinal uses, chemical constituents and pharmacological properties. Pharmaceutical biology, 55(1), 1093-1113.

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  • How to Cite

    Melse Laurence, Y., Shaheen Khan, M., & Sani Sarjadi, M. (2018). Physical Properties and Total Phenolic Content by Different Drying and Extraction Techniques of Clinacanthus Nutans Leaves. International Journal of Engineering & Technology, 7(2.29), 1126-1129. https://doi.org/10.14419/ijet.v7i2.29.15141

    Received date: 2018-07-05

    Accepted date: 2018-07-05

    Published date: 2018-05-22