Optimisation of Gallic Acid and Quercetin Extraction from Phyllanthus Niruri

  • Authors

    • Suok Suok Ling Nguang
    • Yi Ling Yeong
    • Sook Sook Fun Pang
    • Jolius Gimbun
    2018-07-27
    https://doi.org/10.14419/ijet.v7i3.13.16331
  • Phyllanthus niruri, ultrasonic assisted extraction, gallic acid, quercetin, response surface methodology, UPLC.

  • Abstract

    This paper presents an optimization study of gallic acid and queretin extraction from Phyllanthis niruri by using response surface methodology. Gallic acid and quercetin was extracted with the aid of sonication and the polyphenols content was analyzed using Ultra Performance Liquid Chromatography (UPLC). The effect of extraction time, amplitude and ethanol concentration on the yield of gallic acid and quercetin were investigated. The optimum gallic acid yield (10.43 ± 0.28 mg GA/g DW) was found in ethanol concentration of 40.0%, extraction time of 15 minutes and amplitude of 86.85% after solving the regression equation. Meanwhile, the optimum condition to obtain the highest yield of quercetin (17.48 ± 0.21 mg Que/g DW) was found in ethanol concentration of 40.0%, extraction time of 15 minutes and amplitude of 75%. The findings in this work may serve as a useful guide to obtain highest extraction yield of gallic acid and quercetin from Phyllanthus niruri.

     


     

  • References

    1. [1] Boim MA, Heilberg IP & Schor B (2010), Phyllanthus niruri as a promising alternative treatment for nephrolithiasis. Int Braz J Urol 36(6), 657-664.

      [2] Xia Y, Luo H, Liu JP & Gluud C (2011), Phyllanthus species for chronic hepatitis B virus infection Cochrane Database Syst Rev 13(4) doi: 10.1002/14651858.CD008960.pub2.

      [3] Sharma P, Parmar J, Verma P, Sharma P & Goyal PK (2009), Anti-tumor activity of Phyllanthus niruri (a medical plant) on chemical-induced skin carcinogenesis in mice. Asian Pac J Cancer Prec 10(6), pp 1089-1094.

      [4] Markom M, Hasan M, Daud WRW, Singh H & Jahim JM (2007), Extraction of hydrolysable tannins from Phyllanthusniruri linn: Effects of solvents and extraction methods. Separation and Purification Technology 52(3), 487–96.

      [5] Franco J, Sineiro M, Rubilar M, Sanchez M, Jerez M, Pinelo M & Nunez MJ (2008), Polyphenols from plant materials:extraction and antioxidant power. Electronic Journal of Environment, Agriculture and Food Chemistry 7, 3210-3216.

      [6] Pang SF, Yusoff MM & Gimbun J (2014), Assessment of phenolic compounds stability and retention during spray drying of Orthosiphon stamineus extracts. Food Hydrocolloids 37, 159-65.

      [7] Muhamad N, Yusoff MM & Gimbun J (2015), Thermal degradation kimetic of nocotinic acid, pantothenic acid and catechin derived from Averrhoa bilimbi fruits.RSC Advances 5, 74132-74137.

      [8] Mercali GD, Jaeschke DP, Tessaro IC & Marczak LDF (2013), Degradation kinetics of anthocynins in acerola pulp: Comparison between ohmic and conventional heat treatment. Food Chemistry 136, 853-857.

      [9] Murugaiyah V, & Chan KL (2007), Analysis of lignans from Phyllanthus niruri L. in plasma using a simple HPLC method with fluorescence detection and its application in a pharmacokinetic study. Journal of Chromatography 852, 138-144.

      [10] Cuoto AG, Kunzler M, Spaniol B, Magalhaes P, Ortega G & Petrovick P (2013), Chemical and technological evaluation of the Phyllanthus niruri aerial parts as a function of cultivation and harvesting conditions. Brazilian Journal of Pharmacognosy 23(1), pp. 36-43.

      [11] Ghosal S, Veeraragavan M & Kalidindi SR (2013), U.S. Patent No. 20130122119A1. New Brunswick. DC: U.S Patent and Office Trademark Office.

      [12] Annamalai A, & Lakshimi PTV (2009), HPTLC and HPLC Analysis of Bioactive Phyllanthin from Different Organs of Phyllanthus amarus. Asian Journal of Biotechnology 1(4), 154-162.

      [13] Tripathi AK, Verma RK, Gupta AK, Gupta MM & Khanuja SP (2006), Quantitative determination of phyllanthinand hypo­phyllantin in phyllanthus species by high-performance thin layer chromatography. Phytochemistry Analysis 17(6), 394–397.

      [14] Metrouh-Amir H, Durte CM & Maiza F (2015), Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens. Industrial Crops and Product 67, 249-256.

      [15] Pang SF, Lau MJ, Yusoff MM & Gimbun J (2017), Microwave-Irradiation Induced Fast Simultaneous Extraction of Methoxylated and Hydroxylated Phenolic Compounds from Orthosiphon stamineus Leaves. Materials Science Forum 890, 155-158.

      [16] Poh-Hwa T, Yoke-Kqueen C, Indu Bala J & Son R (2011), Bioprotective properties of three Malaysia Phyllanthus species: An investigation of the Antioxidant and Antimicrobial Activities. International Food Research Journal 18, 887-893.

      [17] Tian F, Li B, Ji BP, Yang JH, Zhang GZ, Chen Y & Luo YC (2009), Antioxidant and antimicrobial activities of consecutive extracts from Galla chinesis: The polarity effects the bioactivities. Food Chemistry 113, 173-179.

      [18] Nguang SL, Yeong YL, Pang SF & Gimbun J (2016), Ultrasonic Assisted Extraction on Phenolic and Flavonoid Content from Phyllanthus niruri Plant. Indian Journal of Science and Technology 10(2), 1-5.

  • Downloads

  • How to Cite

    Suok Ling Nguang, S., Ling Yeong, Y., Sook Fun Pang, S., & Gimbun, J. (2018). Optimisation of Gallic Acid and Quercetin Extraction from Phyllanthus Niruri. International Journal of Engineering & Technology, 7(3.13), 90-94. https://doi.org/10.14419/ijet.v7i3.13.16331

    Received date: 2018-07-26

    Accepted date: 2018-07-26

    Published date: 2018-07-27