Statistical Mixture Design: Study of Solvent Performance in Temperature Controlled Microwave Assisted Extraction System on Antioxidant Properties of Nephrolepis biserrata (Schott.) Sw. frond Extract

  • Abstract
  • Keywords
  • References
  • PDF
  • Abstract

    Statistical simplex-centroid design mixtures of water, acetone, acetonitrile, ethanol were used to extract Nephrolepis biserrata (Schott.) SW. frond (NBF) using temperature controlled microwave extraction system (TCMES).  The effects of different solvents and their mixtures on its antioxidant properties were studied. The result shows, the quality of extracted material is found to depend on the solvent proportions according to special cubic model of TPC, TFC and IC50. The binary and quaternary solvents are found to explain the superior extraction results as compared to single solvent for TPC, TFC and IC50.



  • Keywords

    Mixture Design, Nephrolepis biserrata (Schott.) Sw, TPC, TFC, IC50

  • References

      [1] E.D. Neas, and M.J. Collins, “Microwave heating. Theoretical concepts and equipment design, In Kingston, H.M. and Jassie, L.B. (Ed). I Introduction to microwave sample preparation. Theory and practice”. Ame. Chem. Soc. Washington, D.C., Chap. 2, pp. 7-32, 1988.

      [2] L. Perreux, ”A Tentative Rationalization of Microwave Effects In Organic Synthesis According to The Reaction Medium, and Mechanistic Considerations”, Tetrahedron, Vol. 57, pp 9199-9223, 2001.

      [3] V. Mandal, Y. Mohan, and S. Hemalatha, “Microwave Assisted Extraction - An Innovative and Promising Extraction Tool for Medicinal Plant Research”, Pharma. Review. Vol. 1, pp. 7-18, 2007.

      [4] B. Kaufmann, and P. Christen, “Recent extraction techniques for natural products: microwave-assisted extraction and pressurized solvent extraction”, Phytochem. anal, Vol. 13, pp. 105-113, 2002.

      [5] H.M. Kingston, and S. J. Haswell, “Microwave Enhanced Chemistry: Fundamentals Sample Preparation and Applications, American Chemical Society. 1997.

      [6] K. Ganzler, A. Salgo, and K. Valko, “Microwave extraction, a novel sample preparation method for chromatography. J.Chrom. Vol. 371, pp. 299–306, 1986.

      [7] H. Christensen, “Uses of ferns in two indigenous communities in Sarawak, Malaysia”, In R.J. Johns (ed.): Holttum Memorial Volume, Roy. Bot. Gard., Kew (U.K.), pp 177-192, 1997.

      [8] K. Siems, F. Weigt, and E. Wollenweber, “Drimanes from the epicuticular wax of the fern Nephrolepis biserrata”, J. Phytochem. Vol. 33, pp. 89-97. 1996.

      [9] D. Marinova, F. Ribarova, and M. Atanassova, “Total Phenolics And Total Flavonoids In Bulgaria Fruits And Vegetables”, J. Uni. Chem. Tech. and Metal. Vol. 40, pp. 255-260, 2005.

      [10] Braca, De. Nunziatina, L. Tommasi , D.B. Lorenzo, P. Cosimo, P. Matteo, and M. Ivano, ”Antioxidant Principles from Bauhinia tarapotensis”. J. Nat. Pro.Vol. 64, pp.892–895, 2001.

      [11] W.G. Cochran, and G.M. Cox, “Experimental designs”2nd ed. New York, John Wiley & Sons, pp.611, 1992.

      [12] D.C. Mothgomery, “Design and analysis of experiments”. 5thed, New York: John Wiley and Sons, pp.170-210, 2001.

      [13] Y. Yang, and F. Zhang, “Ultrasound-assisted extraction of rutin and quercetin from Euonymus alatus (Thunb.) Sieb”, Ultra. Sonochem., Vol. 15, pp. 308-313, 2008.

      [14] S.J. Kim, H.N, Murthy, E.J. Hahn, H.L. Lee, and K.Y. Paek, “Parameters affecting the extraction of ginsenosides from the adventitious roots of ginseng (Panax ginseng C.A. Meyer). Sep. Pur. Tech., Vol. 56, pp. 401-406, 2007.

      [15] H. Nawaz, J. Shi, G.S. Mittal, and Y. Kakuda, “Extraction of polyphenols from grape seeds and concentration by ultrafiltration” Sep. Pur. Tech., Vol. 48, pp. 176-181, 2006.

      [16] N. Turkmen, F. Sari, and Y.S. Velioglu, “Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin–Ciocalteu methods” Food Chem. Vol. 99, pp. 835-841, 2006.

      [17] J.M. Oespchuck, “A history of microwave-heating applications”, IEEE Trans, Microwave Theory Tech., Vol. 32, pp. 1200–1224, 1984.

      [18] C.R. Strauss, and R.W. Trainor, “Developments in microwave-assisted organic chemistry”, Aust. J. Chem.,Vol. 48, pp.1665-1692, 1995.

      [19] S. Frere, V. Thiéry, and T. Besson, “Microwave acceleration of the Pechmann reaction on graphite:montmorillonite K10: application to the preparation of4-substituted 7-aminocoumarins”, Tetrahedron Lett.,Vol. 42, pp. 2791-2794, 2001.

      [20] R.M. Kelly, and N.A. Rowson, “Microwave Reduction of Oxidized Ilmenite Concentrates”, Minerals Eng., Vol. 8, pp. 1427-1438, November, 1995.

      [21] X. Liao, “Dielectric properties and their application in microwave-assisted organic chemical reactions”, PhD Thesis. McGill University, Canada, 2002.

      [22] H. Yilmaz, “Excess Properties of Alcohol, Water Systems at 298.15 K”, Turk. J. Phys. Vol. 26, pp. 243-246, 2002.

      [23] F. Kormin, N.H. Abdurahman, R.M. Yunus, M. Rivai, “Study the Heating Mechanisms of Temperature Controlled Microwave Closed System (TCMCS),” Intl. J. Eng. Science Inno. Tech, Vol. 2, pp. 417-429, September, 2013.

      [24] R. Yazdanparast. A. Ardestani. “In Vitro Antioxidant and Free Radical Scavenging Activity of Cyperus Rotundus”. J. Med. Food. 10(4): 667- 674, 2007.

      [25] M. .F. A. Ghafar, K. N. Prasad, K.K. Weng and A. Ismail. “ Flavonoid, hesperidine, total phenolic contents and antioxidant activities from Citrus species”African Journal of Biotechnology. Vol. 9(3), pp. 326-330, 2010 .

      [26] M.R. Criado, S.P. Torre, I.R. Pereiro, and R.C. Torrijos, “Optimization of a microwave-assisted derivatization–extraction procedure for the determination of chlorophenols in ash samples”. J. Chrom. A, Vol.1024, pp. 155–163, 2004.

      [27] N. Lay-Keow, and H. Michel, “Effects of moisture content in cigar tobacco on nicotine extraction similarity between Soxhlet and focused open-vessel microwave-assisted techniques” J. Chrom. A, Vol.11, pp. 213–219,2003.




Article ID: 19061
DOI: 10.14419/ijet.v7i3.7.19061

Copyright © 2012-2015 Science Publishing Corporation Inc. All rights reserved.