Canna edulis KERR. and spirulina platensis as a prebiotic

  • Authors

    • Nita Noriko
    • Analekta Tiara Perdana
    • Fadly Wiramandiri
    https://doi.org/10.14419/ijet.v7i3.29.19313
  • Probiotics, Prebiotics, Lactobacillus Bacteria, MRSA Modified

  • Probiotics have benefit on reducing the number of pathogenic bacteria in human the digestive system. One way to keep the amount of probiotics in the human body is to consume prebiotics. One source of prebiotic food is red C. edulis Kerr because contain starch and fiber. S. platensis is a microalgae having 60-70% protein contain and when combined with flour from red tubers C.edulis Kerr will increase nutritional contain. The research about the potency of red tubers Kerr flour and S. platensis powder as prebiotics has done by growing L. casei rhamnosus in De Mann Rogosa and Sharpe Agar (MRSA) as control and control negative without glucose and several modified MRSA. There were glucose MRSA substituted to C.edulis Kerr (MRSA C), peptone to S. platesis (MRSA P) , glucose and peptone to C.edulis Kerr flour and S. platensis (MRSA CP), and also complete flour composite C. edulis, S. platensis and Acalypha indica powder as additive oresevative substantive (MRSA CPA). Indicator potencial flour of Kerr, S. platensis powder in modified medium as prebiotics by calculating Total Plate Count (TPC) methode and test for anti-pathogen bacteria. Gram Test was conducted to make sure the kind of bacteria. The results showed L. casei rhamnosus grew in MRSA and modified medium. The total population of L. casei rhamnosus in MRSA, MRSA C, MRSA CP and MRSA CPA are not significantly (P< 0,05). MRSA P is the highest total of L. casei rhamnosus and when growing in modified MRSA CP can inhibit the growth of E. coli. Gram test bacteria in clear zone showed positif. It indicate L. casei rhamnosus. C. edulis Kerr and S.platensis in modified MRSA have potential as prebiotics by invitro methodology. A. indica in MRSA CPA did not inhibit growth of L. casei rhamnosus.

     

     

  • References

    1. [1] Antarini AAN. 2011. Sinbiotik antara Prebiotik dan Probiotik. J Ilmu Gizi. 2:148-155.

      [2] Cardici BH, Citak S. 2005. A comparison of two methods used for measuring antagonistics activity of lactic acid bacteria. Pakistan J Nutr 4: 237-241.

      [3] Christwardana M, Nur MMA, Hadiyanto. 2013. Spirulina platensis potensinya sebagai bahan pangan fungsional. J Aplikasi Teknologi Pangan. Two 1-4.

      [4] Delgado A, Brito D, Fevereiro P, Peres C, Marques JF. 2001. Antimicrobial activity of L. plantarum, isolated from a traditional lactic acid fermentation of table olives. INRA, EDP Science 81: 203-215.

      [5] Dommels YEM, Kemperman RA, Zebregs YEMP, Draaisma RB. 2009. Survival of Lactobacillus reuteri DSM 17938 and Lactobacilus rhamnosus GG in the human gastrointestinal tract with daily consumption of a low-fat probiotic spread. Appl Environ Microbiol. 75 6198-204.

      [6] Fatmala R. 2015. Pengaruh konsentrasi ektrak etanol kulit manggis (Garcinia mangostana Linn) terhadap daya hambat pertumbuhan bakteri Lactobacillus acidophilus (kajian In Vitro) [Skripsi]. Surakarta: Universitas Muhammadiyah.

      [7] Fitri L dan Yasmin Y. 2011. Isolasi dan Pengamatan Morfologi Koloni Bakteri Kitinolitik. Jurnal Ilmiah Pendidikan Biologi 3(2): 20-25.

      [8] Krisnayudha K. 2007. Mempelajari potensi garut dan ganyong untuk mendukung pertumbuhan bakteri asam laktat [Skripsi]. Bogor: Institut Pertanian Bogor.

      [9] Martini D. 2013. Daya pembengkakan-SwellingSwelling power) granula campuran tepung ganyong (Canna edulis ker.) dan tepung terigu terhadap elastisitas dan daya terima mie basah [Skripsi]. Surakarta: Universitas Muhammadiyah Surakarta.

      [10] Maunatin A dan Khanifa. 2012. Uji potensi probiotik Lactobacillus planetarium secara in-vitro. J. Alchemy 2: 26- 34.

      [11] Muchtadi. 2010. Ilmu Pengetahuan Bahan Pangan. Bogor: Alfabeta CV.

      [12] Noriko N. dan Swandari R. 2013. Ganyong dan Spirulina sebagai Produk Pangan Alternatif. J Matematika, Sains, dan Teknologi. Vol 4, D.121-D.12.

      [13] Pambudi A, Syaefudin, Noriko N, Swandari R, Azura PR. 2014. Identifikasi bioaktif golongan flavonoid tanaman anting- anting (Acalypha indica L.). J Al- Azhar Indones Seri Sains Teknol 2: 178- 187.

      [14] Pelezar JM, Micheal J, Chan EC. 2008. Dasar-dasar Mikrobiologi. Jilid 1. Terjemahan. Jakarta: Universitas Indonesia Press.

      [15] Primasari N. 2015. Identifikasi kandungan flavonoid pada daun dan umbi ganyong putih Canna edulis Kerr [Skripsi]. Jakarta: Universitas Al- Azhar Indonesia.

      [16] Rachmawati I, Suratno, Setyaningsih R. 2005. Uji Antibakteri bakteri asam laktat asal asinan sawi terhadap bakteri patogen. Jurnal Bioteknologi 2: 43-48.

      [17] Richana, N. dan Sunarti, T.C. 2004. Karakterisasi Sifat Fisiokimia Tepung Umbi Dan Tepung Pati dari Umbi Ganyong, Suweg, Ubi Kelapa Dan Gembili. J.Pascapanen 1(1) 2004: 29-37.

      [18] Sabir A. 2005. Aktivitas Antibakteri Flavonoid Propolis Trigona sp. Terhadap Bakteri Streptococcus mutans (in vitro). J Kedokteran Gigi 38: 135-141.

      [19] Sari R. 2012. Karakterisasi bakteri probiotik yang berasal dari saluran pencernaan ayam pedaging [skripsi]. Makassar: Universitas Hasanuddin.

      [20] Sutarman. 2000. Kultur Media Bakteri. Balai Penelitian Veteriner. Temu Teknis Fungsional non-Peneliti.

      [21] Tamime AY, Robinson RK. 2007. Yoghurt, Science, and Technology. ED ke-3. New York: CRC Pr.

      [22] Toma MM, Pokrotnieks J. 2006. Probiotics as functional food: microbiological and media aspects. Acta Universitatis Latviensis.710: 117-120.

      [23] Widianigsih, Ridho A, Hartati R, Harmoko. 2008. Kandungan nutrisi Spirulina plantesis yang dikultur pada media berbeda. Jurnal Ilmu Kelautan 13: 167- 170.

      [24] Yunita M, Hendrawan Y, Yulianingsih R. 2015. Analisis kuantitatif mikrobiologi pada makanan penerbangan (Aerofood ACS) Garuda Indonesia berdasarkan TPC (Total Plate Count) dengan metode Pour Plate. J. Keteknikan Pertanian Tropis dan Biosistem 3: 237-248.

      [25] Yusmarini, Indrati R, Utami T, Marsono Y. 2009. Isolasi dan identifikasi bakteri asam laktat proteolitik dari susu kedelai yang terfermentasi spontan. Jurnal Natur Indonesia 12: 28.

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    Noriko, N., Tiara Perdana, A., & Wiramandiri, F. (2018). Canna edulis KERR. and spirulina platensis as a prebiotic. International Journal of Engineering & Technology, 7(3.29), 564-567. https://doi.org/10.14419/ijet.v7i3.29.19313