The effects of microbial fermentation on antibacterial activity of seaweed (Kappaphycus alvarezii) extracts

  • Authors

    • Norakma M.N
    • Zaibunnisa A.H.
    • Wan Razarinah W.A.R
    2018-11-27
    https://doi.org/10.14419/ijet.v7i4.18.21928
  • Antibacterial, Aspergillus oryzae, Kappaphycus alvarezii, microbial fermentation, red algae
  • Abstract

    In the present study, Kappaphycus alvarezii one of the most abundant red algae in Malaysia was fermented with Aspergillus oryzae at 30oC for six days with 70% initial moisture content. Total phenolic content and antibacterial activity of fermented seaweed extracts was determined and compared to the non-fermented counterparts. It is found that total phenolic content increased as the fermentation progressed. Green variety fermented for four days exhibited the highest phenolic content (10.022 mg GAE/g). Meanwhile, fermented seaweed extracts tested for antibacterial activity showed that antibacterial activity of fermented seaweed against six different bacteria increased as the fermentation day increased. Green and Yellow variety fermented for six days demonstrated the highest antimicrobial activity (7 mm) against MRSA and Salmonella respectively. pH of fermented seaweed extracts increased with the increase of fermentation day and it might be related with the antimicrobial susceptibility which greater at increasing pH value.

     

     

  • References

    1. [1] Abirami RG & Kowsalya S (2011), Nutrient and nutraceutical potentials of seaweed biomass Ulva lactuca and Kappaphycus alvarezii. Journal of Agricultural Sciences and Technology, 5, 109-115.

      [2] Pérez MJ, Falqué E & Domínguez H (2016), Antimicrobial action of compounds from marine seaweed. Marine Drugs, 14(3), 52.

      [3] Yan S & Asmah R (2010), Comparison of total phenolic contents and antioxidants activities of turmeric leaf, pandan leaf and torch ginger flower. International Food Research Journal, 17, 417-423.

      [4] Namvar F, Mohamed S, Fard SG, Behravan J, Mustapha NM, Alitheen NBM, et al. (2011), Polyphenol-rich seaweed (Eucheuma cottonii) extract suppresses breast tumour via hormone modulation and apoptosis induction. Food Chemistry, 130(2): 376-82.

      [5] Dulf FV, Vodnar DC & Socaciu C (2016), Effects of solid-state fermentation with two filamentous fungi on the total phenolic contents, flavonoids, antioxidant activities and lipid fractions of plum fruit (Prunus domestica L.) by-products. Food Chemistry, 209, 27–36.

      [6] Ajila CM, Gassara F, Brar SK, Verma M, Tyagi RD & Valéro JR (2012), Polyphenolic antioxidant mobilization in apple pomace by different methods of solid-state fermentation and evaluation of its antioxidant activity. Food Bioprocess Technology, 5, 2697–2707.

      [7] Machida M, Yamada O & Gomi K (2008), Genomics of Aspergillus oryzae: learning from the history of koji mold and exploration of its future, DNA Research, 15(4), 173–183.

      [8] Ellaiah P, Adinarayana K, Bha Y, Padmaja P & Srini B (2002), Optimization of process parameters for glucoamylase production under solid state fermentation by a newly isolated Aspergillus species. Process Biochemistry, 38, 615–620.

      [9] Burkholder PR, Burkholder LM & Almodovar LR (1960), Antibiotic activity of some marine algae of Puerto Rico. Bot. Marina, 2: 149-156.

      [10] Jeyanthi RL, Dhanalakshmi V & Chandra S (2012), Antibacterial activity of Sargassum Ilicifolium and Kappaphycus alvarezii, Journal of Chemical and Pharmaceutical Research, 4(1), 700-705.

      [11] Lalitha MK, Manayani DJ, Priya L, Jesudason MV, Thomas K, & Steinhoff MC (1997), E-test as an alternative to conventional MIC determination for surveillance of drug resistant S.pneumoniae. Indian Journal of Medical Res.,106; 500-503.

      [12] Ballantine DL, Gerwick WH, Velez SM, Alexander E & Guevara P (1987), Antibiotic activity of lipid-soluble extracts from Caribbean marine algae. In Hydrobiologia, 151, 463–469.

      [13] Yang L, Kunjie W, Hong L, John DD & Peter AC (2014), The influence of urinary pH on antibiotic efficacy against bacterial uropathogens. Journal of Urology, 84, 731.

      [14] Bae HN & Kim YM (2010), Improvement of the functional qualities of sea tangle extract through fermentation by Aspergillus oryzae. Fish Aquatic Science, 13, 12–17.

      [15] Chancharoonpong C, Hsieh PC, Sheu SC (2012), Production of enzyme and growth of Aspergillus oryzae S. on soybean koji. International Journal of Bioscience, Biochemistry and Bioinformatics, 2(4), 228 – 231.

      [16] Liang Y, Pan L & Lin Y (2009), Analysis of extracelular proteins of Aspergillus oryzae grown on soy sauce koji, Bioscience, Biotechnology Biochemistry, 73(1): 192-195.

      [17] Sjostrom JE & Larsson H (1996), Factors affecting growth and antibiotic susceptibility of Helicobacter pylori: effect of pH and urea on the survival of a wild-type strain and a urease-deficient mutant. Journal of Medical Microbiology, 44, 425-433.

      [18] Amorati R, Pedulli GF, Gabrini L, Zambonin L & Landi L. (2006). Solvent and pH effects on the antioxidant activity of caffeic and other phenolic acids. Journal of Agricultural and Food Chemistry, 54(8), 2932 - 2937.

      [19] Pushparaj A, Raubbin RS & Balasankar T (2014), Antibacterial activity of Kappaphycus alvarezii and Ulva lactuca extracts against human pathogenic bacteria. International Journal of Current Microbiology and Applied Sciences, 3(1), 432 – 436.

      [20] Cox S, Ghannam-Abu N & Gupta S (2010), An assessment of the antioxidant and antimicrobial activity of six species of edible red seaweeds. Int Food Res J,17: 205-220.

      [21] Jassbi AR, Mohabati M, Eslami S, Sohrabipour J & Miri R (2013), Biological activity and chemical constituents of red and brown algae from the Persian Gulf. Iran J Pharm Res,12(3): 339-348.

      [22] Alexander C & Rietchel ET (2001), Bacterial lipopolysaccharides and innate immunity. J Endotoxin Resist, 7, 167-202.

      [23] Kaplan M (2000), The problem with gram-negative bacteria. Herb Care Collection. The Merck Manual: Home Edition, 17, 177.

      [24] Raetz CR & Whitfield C (2002), Lipopolysaccharide endotoxins. Annu Rev Biochem, 71, 635-700.

      [25] Rosenfeld Y & Shai Y(2006), Lipopolysaccharide (Endotoxin)-host defense antibacterial peptides interactions: role in bacterial resistance and prevention of sepsis. Biochim Biophys Acta Rev Biomembr, 1758(9): 1513-22.

      [26] Bhanja T, Kumari A & Banerjee R (2009), Enrichment of phenolics and free radical scavenging property of wheat koji prepared with two filamentous fungi. Bioresource Technology, 100(11), 2861-2866.

      [27] Cho KM, Hong SY, Math RK, Lee JH, Kambiranda DM, Kim JM, et al. (2009). Biotransformation of phenolics (isoflavones, flavanols and phenolic acids) during the fermentation of cheonggukjang by Bacillus pumilus HY1. Food Chemistry, 114, 413–419.

      [28] Puupponen-Pimiä R, Nohynek L, Meier C, Kähkönen M., Heinonen M, Hopia A, et al. (2001), Antimicrobial properties of phenolic compounds from berries. Journal of Applied Microbiology, 90, 494–507.

      [29] Madhavarani A & Ramanibai R (2014), In-vitro antibacterial activity of Kappaphycus alvarezii extracts collected from Mandapam Coast, Rameswaram, Tamil Nadu. International Journal of Innovative Research in Science, Engineering and Technology, 3(1), 90-99.

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  • How to Cite

    M.N, N., A.H., Z., & Razarinah W.A.R, W. (2018). The effects of microbial fermentation on antibacterial activity of seaweed (Kappaphycus alvarezii) extracts. International Journal of Engineering & Technology, 7(4.18), 247-251. https://doi.org/10.14419/ijet.v7i4.18.21928

    Received date: 2018-11-27

    Accepted date: 2018-11-27

    Published date: 2018-11-27