Extraction and Characterization of Chitin from Leucaena Leococephala

  • Authors

    • Noor Harliza Abd Razak
    • Shareena Shahira Tajul Arus
    • N. A . Zainuddin
    2018-11-27
    https://doi.org/10.14419/ijet.v7i4.18.21938
  • chitin, elemental analysis, leucaena leucocephala pods, TGA

  • Abstract

    In this research, a new source of chitin was proposed. Chitin was extracted leucaena leucocephala pods using NaOH and HCl solutions for deproteination and demineralisation, respectively. The purposes of this research were to characterize chitin by extraction of LL pods at different length and aging with 6M HCL and 12M of NaOH by using TGA and elemental Analysis. Thermal property characterization of chitin from leucaena leucocephala pods (LL) extraction was obtained by using thermogravimetric analysis (TGA) and elemental analysis. The TGA and Elemental Analysis then tested the prepared samples for the thermal property. For TGA, the results show development stage at LL (Set 6) has the highest thermal stability. However, for elemental analysis, the results show that the degree of deacetylation is the highest for carbon-nitrogen ratio at young LL because of high protein content. Furthermore, it was found that the DA decreased with an increasing thermal property.

     

     

  • References

    1. 1] P. Kumar Dutta, J. Dutta, and V. S. Tripathi, “Chitin and chitosan: Chemistry, properties and applications,†J. Sci. Ind. Res., vol. 63, pp. 20–31, 2004.

      [2] A. Hassainia, H. Satha, and S. Boufi, “Chitin from Agaricus bisporus: Extraction and characterization,†Int. J. Biol. Macromol., vol. 117, pp. 1334–1342, 2018.

      [3] H.-S. Jung, M. H. Kim, J. Y. Shin, S. R. Park, J.-Y. Jung, and W. H. Park, “Electrospinning and wound healing activity of β-chitin extracted from cuttlefish bone,†Carbohydr. Polym., vol. 193, pp. 205–211, 2018.

      [4] F. A. Al Sagheer, M. A. Al-Sughayer, S. Muslim, and M. Z. Elsabee, “Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf,†Carbohydr. Polym., vol. 77, pp. 410–419, 2009.

      [5] N. Mittal, P. Mattu, and G. Kaur, “Extraction and derivatization of Leucaena leucocephala (Lam.) galactomannan: Optimization and characterization,†Int. J. Biol. Macromol., vol. 92, pp. 831–841, 2016.

      [6] S. Kumari and P. Kumar, “Extraction and Characterization of Chitin and Chitosan from (Labeo rohit) Fish Scales,†Procedia Mater. Sci., vol. 6, no. Icmpc, pp. 482–489, 2014.

      [7] M. C. Gortari and R. A. Hours, “Biotechnological processes for chitin recovery out of crustacean waste: A mini-review,†Electron. J. Biotechnol., vol. 16, no. 3, 2013.

      [8] A. Singhal, M. Kumar, M. Bhattacharya, N. Kumari, P. K. Jha, D. K. Chauhan, and I. S. Thakur, “Pretreatment of Leucaena leucocephala wood by acidified glycerol: optimization, severity index and correlation analysis,†Bioresour. Technol., vol. 265, pp. 214–223, 2018.

      [9] N. Mittal, P. Mattu, and G. Kaur, “Extraction and derivatization of Leucaena leucocephala (Lam.) galactomannan: Optimization and characterization,†Int. J. Biol. Macromol., vol. 92, pp. 831–841, 2016.

      [10] Z. Ilham, H. Hamidon, N. A. Rosji, N. Ramli, and N. Osman, “Extraction and Quantification of Toxic Compound Mimosine from Leucaena Leucocephala Leaves,†Procedia Chem., vol. 16, pp. 164–170, 2015.

      [11] H. M. Shelton and J. L. Brewbaker, “Leucaena leucocephala - the most widely used forage tree legume.,†in Forage tree legumes in tropical agriculture., Wallingford: CAB INTERNATIONAL, 1994, pp. 15–29.

      [12] V. Jayanthy, R. Geetha, R. Rajendran, and P. Prabhavathi, “Phytoremediation of dye contaminated soil by Leucaena leucocephala (subabul) seed and growth assessment of Vigna radiata in the remediated soil,†Saudi J. Biol. Sci., vol. 21, no. 4, pp. 324–333, 2014.

      [13] K. A. and C. O. 2018: Atalay A I, Ozkan C O, Kaya E, “Chemical composition,nutritive value and rumen methane potential of some legume tree pods.,†Livest. Res. Rural Dev., vol. 30, no. 92, 2018.

      [14] R. L. Lavall, O. B. G. Assis, and S. P. Campana-Filho, “b-Chitin from the pens of Loligo sp.: Extraction and characterization,†2006.

      [15] M. Kaya, T. Baran, S. Erdoğan, A. Menteş, M. A. Özüsağlam, and Y. S. Çakmak, “Physicochemical comparison of chitin and chitosan obtained from larvae and adult Colorado potato beetle (Leptinotarsa decemlineata),†Mater. Sci. Eng. C, vol. 45, pp. 72–81, 2014.

      [16] R. M. Abdel-Rahman, R. Hrdina, A. M. Abdel-Mohsen, M. M. G. Fouda, A. Y. Soliman, F. K. Mohamed, K. Mohsin, and T. D. Pinto, “Chitin and chitosan from Brazilian Atlantic Coast: Isolation, characterization and antibacterial activity,†Int. J. Biol. Ma,†Int. J. Biol. Macromol., vol. 80, pp. 107–20, Sep. 2015.

      [17] W. Sajomsang and P. Gonil, “Preparation and characterization of chitin from cicada sloughs,†Mater. Sci. Eng. C, vol. 30, no. 3, pp. 357–363, 2010.

      [18] E. Layne, “Spectrophotometric and turbidimetric methods for measuring proteins,†Methods Enzymol., vol. 3, no. C, pp. 447–454, 1957.

      [19] I. Younes, S. Hajji, M. Rinaudo, M. Chaabouni, K. Jellouli, and M. Nasri, “Optimization of proteins and minerals removal from shrimp shells to produce highly acetylated chitin.,†Int. J. Biol. Macromol., vol. 84, pp. 246–53, Mar. 2016.

      [20] J. Domínguez-Robles, T. Tamminen, T. Liitiä, M. S. Peresin, A. Rodríguez, and A. S. Jääskeläinen, “Aqueous acetone fractionation of kraft, organosolv and soda lignins,†Int. J. Biol. Macromol., 2017.

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  • How to Cite

    Harliza Abd Razak, N., Shahira Tajul Arus, S., & A . Zainuddin, N. (2018). Extraction and Characterization of Chitin from Leucaena Leococephala. International Journal of Engineering & Technology, 7(4.18), 297-300. https://doi.org/10.14419/ijet.v7i4.18.21938

    Received date: 2018-11-27

    Accepted date: 2018-11-27

    Published date: 2018-11-27