An Investigation of Attached Biomass on Bio-Media using Scanning Electron Microscopy

  • Authors

    • Gasim Hayder
    • Puniyarasen Perumulselum
    2018-11-30
    https://doi.org/10.14419/ijet.v7i4.35.22900
  • Attached growth, wastewater treatment, Bio-media, Scanning electron microscopy
  • Biological film that attached to bio-media in attached growth wastewater treatment processes needs to be monitored and evaluated as it reflects the performance of the plant. Using Scanning Electron Microscopy (SEM) is an effective way for the examination of biofilms. Three different types of bio-media (bio-balls) were used to study the biofilm thickness using SEM. They are fin ball, spike ball and cage ball. Each type of bio-balls was cut into pieces since the size of SEM sample must be less than 25 mm. The fin ball was cut into three pieces, the spike ball was cut into two pieces and the cage ball was cut into one piece. Only the non-identical parts from the bio-balls were chosen for SEM test. The cut pieces with biofilm growth were overnight dehydrated in laboratory oven before bring to the SEM for measuring and analysis of the thickness of biofilm growth. Based on average biofilm thickness of the cut pieces extracted from SEM test, cage ball and fin ball recorded the highest biofilm while spike ball has the lowest biofilm growth on it.

  • References

    1. [1] Gasim, H., Megat, A. R. M. M. A., & Shamsul, R. M. K. (2015). Treatment of petroleum refinery wastewater using extended aeration activated sludge. In International Journal of Engineering Research in Africa (Vol. 13, pp. 1-7). Trans Tech Publications.

      [2] Sehar, S., & Naz, I. (2016). Role of the biofilms in wastewater treatment. In Microbial Biofilms-Importance and Applications. InTech.

      [3] Diao, H. F., Li, X. Y., Gu, J. D., Shi, H. C., & Xie, Z. M. (2004). Electron microscopic investigation of the bactericidal action of electrochemical disinfection in comparison with chlorination, ozonation and Fenton reaction. Process biochemistry, 39(11), 1421-1426.

      [4] Bayarassou, M., Zidani, M., & Farh, H. (2018). Microstructural Evolution and Mechanical Properties during Homogenization and Ageing Treatment of Al-Mg-Si Alloy Wire Cold Drawn. In International Journal of Engineering Research in Africa (Vol. 36, pp. 60-68). Trans Tech Publications.

      [5] Drouiche, N., Aoudj, S., Hecini, M., Ghaffour, N., Lounici, H., & Mameri, N. (2009). Study on the treatment of photovoltaic wastewater using electrocoagulation: Fluoride removal with aluminium electrodes—Characteristics of products. Journal of Hazardous Materials, 169(1-3), 65-69.

      [6] Goel, J., Kadirvelu, K., Rajagopal, C., & Garg, V. K. (2005). Removal of lead (II) by adsorption using treated granular activated carbon: batch and column studies. Journal of hazardous materials, 125(1-3), 211-220.

      [7] Eighmy, T. T., Maratea, D., & Bishop, P. L. (1983). Electron microscopic examination of wastewater biofilm formation and structural components. Applied and environmental microbiology, 45(6), 1921-1931.

      [8] Al-Ghouti, M. A., Khraisheh, M. A. M., Allen, S. J., & Ahmad, M. N. (2003). The removal of dyes from textile wastewater: a study of the physical characteristics and adsorption mechanisms of diatomaceous earth. Journal of Environmental Management, 69(3), 229-238.

      [9] Mohan, S. V., Rao, N. C., Prasad, K. K., Madhavi, B. T. V., & Sharma, P. N. (2005). Treatment of complex chemical wastewater in a sequencing batch reactor (SBR) with an aerobic suspended growth configuration. Process Biochemistry, 40(5), 1501-1508.

      [10] Cha, J., Choi, S., Yu, H., Kim, H., & Kim, C. (2010). Directly applicable microbial fuel cells in aeration tank for wastewater treatment. Bioelectrochemistry, 78(1), 72-79.

  • Downloads

  • How to Cite

    Hayder, G., & Perumulselum, P. (2018). An Investigation of Attached Biomass on Bio-Media using Scanning Electron Microscopy. International Journal of Engineering & Technology, 7(4.35), 509-512. https://doi.org/10.14419/ijet.v7i4.35.22900