Immunoferment, Cytomorphological, Cytochemical Parameters of Fish as Ecological Indicators of Resistance to Helminthes, Passed by Fish-Eating Birds

 
 
 
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  • Abstract


    A search for indicators reflecting resistance of fish to helminth infections passed by fish-eating birds has been carried out. A decrease in the concentration of lysozyme by 13.5 % and an increase in hemagglutinin by 7.7 % for the bighead carp (Hypophthalmichthys nobilis), 19.9 % and 7.4 % correspondingly for scaly carp (Cyprinus carpio) and 18.6 % and 11.4 %, respectively for white amur (Ctenopharyngodon idela) identify the fish at risk for the incidence of helminths of Trematoda class (Posthodiplostomum brevicaudatum and Diplostomum spathaceum) and Cestoidea class (Pseudophyllidea). The diffuse-gel method revealed that the dynamics of lysozyme marks resistance of fish to invasions. A decrease in lysozyme indices in the kidney of a bighead carp by 30.1 %, that of a scaly carp by 10.9 %, and that of a grass carp by 6.6 % is an indicator of a decrease in the resistance of the fish to helminths. The positive pool of polysaccharide granulation in the cytoplasm of fish lymphocytes detected by periodic acid Schiff reaction attributes them to the risk group for helminth infections passed by fish-eating birds. A dangerous tendency to decrease resistance to invasions is precipitation of large glycogen granules in lymphocytes of silver carp (up to 2.7 %), scaly carp (up to 2.4 %) and white amur (up to 2.9 %), as well as small polysaccharide inclusions up to 24 %, 54 % and 43 % respectively. Among cytomorphologic markers of fish resistance to helminth infections, an increase (2-4 times) in the number of lymphocytes with caudate nuclei, micronuclei, and chromosome aberrations of the dicentric type is benchmark.

     

     


  • Keywords


    resistance to helminthiasis, fish-eating birds, scaly carp, silver carp, white amur.

  • References


      [1] Aleksandrova M.A. Analysis of environmental problems of the barents sea and their influence on the condition of the sanitary and nestles stock of the main wealth of barents sea north-waterstone of the arctic cod. Modern Science Success. 2017. Vol. 2. Issue 12. Pp. 153-156.

      [2] Baranova T.V. Evaluation of the environment at betula pendula cytogenetic parameters. Modern Science Success. 2017. Vol. 1. Issue 9. Pp. 97-104.

      [3] Vikhman, A.A. 'System analysis of immunophysiological reactivity of fish in aquaculture', M., Forwarding agent, 1996, 176 p.

      [4] Vikhman, A.A. and Generalova L.P. 'Guidelines for the quantitative analysis of humoral factors of resistance in organs and tissues of fish', M., VNIIPRH, 1991, 20 p.

      [5] Vikhman A.A., Sitnova O.V., Generalova L.P., Shart L.A. and Borschev V.N. 'On the methods of studying the variability of the immunophysiological reactivity of fish. The development of aquaculture in inland water bodies', M., Moscow University Press, 1995, pp. 58-59.

      [6] Gabysheva N.S., Protopopova A.V., Sorokopudov V.N. Gene pool of ribes nigrum l. on stability to illnesses and wreckers in the conditions of Yakutia. Modern Science Success. 2017. Vol. 1. Issue 9. Pp. 174-180.

      [7] Generalova L.P. and Sitnova O.V. 'Methods to determine lysozyme by liquid-gel method', M., VNIIPRH, 1994, 3 p.

      [8] Golovina N.A., Romanova N.N. and Golovin P.P. 'Ecologo-faunistic analysis of parasites of fish of the Belgorod and Starooskolsky reservoirs', Scientific statements of Belgorod State University, 2017.

      [9] Golovina N.A. and Romanova N.N. 'On the occurrence of Pseudamphistomum truncatum (Rudolphi, 1919) in carp fish in water bodies of central Russia'. In the book: Parasitology in the changing world. Proceedings of the 5th Congress of the Parasitological Society of the Russian Academy of Science (Novosibirsk, September 23-26, 2013), Novosibirsk, 2013, 54.

      [10] Dorovskikh G.N. 'Theoretical and methodological approaches to the study of component communities of freshwater fish parasites'. In the book: Biodiversity of the European North. Abstracts of international scientific conferences, Petrazovodsk, 2001, 57-58.

      [11] Korovushkin A.A. and Nefedova S.A. 'The current state and prospects of commercial aquaculture development', In coll. of the national conference "Innovative development of the modern agro-industrial complex of Russia", FSBEI HE RSATU, December 12, 2016, pp. 360-362.

      [12] Nefedova S.A., Korovushkin A.A., Minin D.G., Shashurina E.A., Afanasyev M.Yu. and Ipatov I.A. 'Biotesting techniques with the use of aquaculture representatives as test objects', Agrarian Russia, Moscow, Folium, 2015, No. 4, pp. 35-39.

      [13] Nefedova S.A., Korovushkin A.A., Minin D.G., Zutova L.B. and Ipatov I.A. 'On the issue of selecting test-sensitive hydrobionts for water bio-testing in laboratory, industrial and natural conditions', Theoretical and Applied Ecology, 2014, No. 3.

      [14] Nefedova S.A., Korovushkin A.A., Shashurina E.A., Minin D.G. and Ipatov I.A. 'To method of biotesting in fish farming', Scientific support of innovative development of the agroindustrial complex. In coll. of scientific papers on the results of the international scientific-practical conference of the faculty, January 29-31, St. Petersburg, Publishing House of St. Petersburg State Agrarian University, 2015, pp. 183-189.

      [15] Nefedova S.A. and Korovushkin A.A. 'Physiological and cytomorphological parameters of animals in various environmental conditions of habitat' (methodology recommendations), Ryazan, RSATU publishing house, 2011, 20 p.

      [16] Novak A.I. 'Invasions of fish in water bodies with different environmental conditions', Russian Journal of Parasitology, (2), 2010, 6-10.

      [17] Platonov T.A., Kuzmina N.V. and Nyukkanov A.N. 'Parasites of Leuciscus Baicalensis (D.) and Esox Lucius (L.) of the middle course of the Lena river and its tributary Vilyuy in the environmental conditions of anthropopression', Natural resources of the Arctic and Subarctic, 2014.

      [18] Romanova N.N., Golovina N.A., Listopadov A.A. and Sekhina O.V. 'Ichthyocotylurus erraticus (family Strigeidae) infection of carp fish in inland waters of the central zone of the Russian Federation'. In the book "Proceedings of the center of parasitology", Center for Parasitology of Institute of Ecology and Evolution Named after A.N. Severtsev, RAS, vol. XLVIII, M., Association of scientific publications KMK, 2014, 75-77.

      [19] Romanova N.N., Golovina N.A., Listopadov A.A. and Sekhina O.V. 'Ecologo-faunistic studies of parasites of fish from the Belgorod reservoir'. In book: "Fishery reservoirs of Russia", Proceedings of the international scientific conference dedicated to the 100th anniversary of GOS-NIORH, St. Petersburg, 2014, 685-693.

      [20] Sitnova O.V. 'Resistance and immunological reactivity of paddlefish (Polyodon spathula (Walb.)) when industrial breeding', synopsis of a thesis of Candidate of Biological Science: 03.00.10, Moscow, 2000, 143 p.

      [21] MU 3.3.2.1758-03 'Methods for determining the quality indicators of immuno-biological preparations for prevention and diagnosis of influenza'.

      [22] Soodeh Shokrolahi, Seyed Mehdi Hosseinifard, Mohammad Raza Youssefi, Mina Sadough Evaluation of parasite fauna in Fish of Alborz Dam // J Parasit Dis. 2016 Mar; 40(1): 129–131. Published online 2014 Jun 22.

      [23] Mehdipour M, Barzegar M, Jalali B. Evaluation of monogeneans parasites of the fish gill in Zayandeh River. Iran // J Veterinary Sci 1(2), 2002.


 

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Article ID: 23748
 
DOI: 10.14419/ijet.v7i4.36.23748




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