Antimicrbial Effect of Diode Laser and Biossynthesis Silver Nanoparticles on Bactrium Staphylococcus Aureus in Vitro

  • Authors

    • Raad Sh. Alnayli
    • Adnan H. Al Hamadani
    • Safaa A. Yaseen
    2018-12-09
    https://doi.org/10.14419/ijet.v7i4.36.23789
  • Diode laser, AgNPs, Nanoparticles, S. aureus

  • Abstract

    The present work was aimed to illustrate how the use of Diode laser or silver nanoparticles (AgNPs) each of them individually, as an antibacterial agent against  Staphylococcus aureus (S. aureus) as well as study the effective result from the absorption of laser energy by these nanoparticles to kill or inhibition bacterial growth. The silver nanoparticles are prepared by biological method. S. aureus are isolated and identified in the Central Health Laboratory in Al Najaf city. Nanoparticles are tested against S. aureus cultured on Muller Hinton agar but Diode laser or laser with nanoparticles are tested against S. aureus cultured in Nutrient broth. S. aureus are irradiating by Diode laser with different irradiation times, moreover there are different concentrations of AgNPs have been employed for killing and inhibition bacterial growth. Results showed that S. aureus is affected by AgNPs (mainly highest concentration of nanoparticles) also detected that decrease bacterial availability with increase time of irradiation to laser.

     

  • References

    1. [1] Kolar M, Urbanek K, Latal T. Antibiotic selective pressure and development of bacterial resistance. Int J Antimicrob 2001;17: 357-63.

      [2] Firdhouse M, Lalitha, P. Biosynthesis of Silver Nanoparticles and Its Applications. J of Nanotech 2015:1-19.

      [3] Chwalibog A, Sawosz E, Hotowy A, Szeliga J, Mitura S, Mitura K et al. Visualization of interaction between inorganic nano-particles and bacteria or fungi. Int J of Nanomed 2010; 5:1085-94.

      [4] Pal S, Tak, Y, Song J. Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli. Appl Environ Microbiol J 2007;73: 1712–20.

      [5] Thomas R, Janardhanan A, Varghese R, Soniya E, Mathew J, Radhakrishnan E. Antibacterial properties of silver nanoparticles synthesized by marine Ochrobactrum sp. Brazilian J of Microbio 2014; 45:1221-7.

      [6] Rathod S, Mayuresh S, Shrikant J, Kshirsagar R. Effects of He-Ne laser irradiation on Escherichia coli and Bacillus subtilis Int J of Basic and Applied 2012;2: 2249-52.

      [7] Mahmood M. The antibacterial effect of silver nanoparticles on some bacterial pathogens. Iraqi J of Physics 2012;10:56-61.

      [8] Poulose S, Panda T, Nair P, Th´eodore T. Biosynthesis of silver nanoparticles. J of Nanosci and Nanotech 2014;14: 2038–49.

      [9] Marambio-Jones C, Hoek E. A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J of Nanoparticle Res 2010;12:1531–51.

      [10] Arya V, Komal R, Kaur M, Goyal A. Silver nanoparticles as a potent antimicrobial agent: a review. Pharmacology online 2011;3:118–24.

      [11] Stadler I, Lanzafame R, Oskoui P, Zhang R, Coleman J, Whittaker M. Alteration of skin temperature during low-level laser irradiation at 830 nm in a mouse model. Photomed Laser Surg 2004: 227-31.

      [12] Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent:a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 2004, 275:177 - 82.

      [13] Theivasanthi T, Alagar M. Anti-bacterial Studies of Silver Nanoparticles. Department of Physics, PACR Polytechnic College, India 2010:1-5.

      [14] Wajih1 Z, Al-dahan1 Z, Ayyad A. Antimicrobial Effects of Silver Nanoparticles Produced by Laser Ablation. Iraqi J of Sci. 2015; 56: 2170-86.

      [15] Amro N, Kotra L, Wadu-Mesthrige K, Bulychev A, Mobashery S, Liu G. High-resolution atomic force microscopy studies of the Escherichia coli outer membrane: structural basis for permeability. Langmuir 2000;16: 2789- 96.

      [16] Guzman M, Dille J, Godet S. Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria. Nanotechnology 2012;8: 37–45.[17] Dakhil A. Biosynthesis of silver nanoparticle (AgNPs) using Lactobacillus and their effects on oxidative stress biomarkers in rats. J of King Saud University- Science 2017; http://dx.doi.org/10.1016/j.jksus.2017.05.013.

      [18] Al-Nori T. Antibacterial activity of Silver and GoldNanoparticles against Streptococcus, Staphylococcus aureus and E.coli . Al- Mustansiriya J. Sci, 2012;23:1-10.

      [19] Ismail M, Waleed S, Jabbar F, Ibrahim K. Effect of Diode Laser (805) nm on alpha-toxin production and antibiotic sensitivity of Staphylococcus aureus. Iraqi J of Science, 2012;53: 755-9.

      [20] Rassam Y. The effect of laser light on virulence factors and antibiotic susceptibility of locally isolated pseudomonas aeruginosa. J Appl. Sci. Res 2010 6: 1298-302.

      [21] Al-Azawi R. Physiological and Genetic study of X-ray effect on Streptococcus spp. Isolated from patients and X-ray exposed technicians. M.Sc. thesis, College of Science. University of Al-Nahrain 2012.

      [22] Hassan S. Physical damage to the bacteria using a combination of laser energy and absorbing nanoparticles. Advances in Environmental Biology 2015;9: 288-94.

  • Downloads

  • How to Cite

    Sh. Alnayli, R., H. Al Hamadani, A., & A. Yaseen, S. (2018). Antimicrbial Effect of Diode Laser and Biossynthesis Silver Nanoparticles on Bactrium Staphylococcus Aureus in Vitro. International Journal of Engineering & Technology, 7(4.36), 290-292. https://doi.org/10.14419/ijet.v7i4.36.23789

    Received date: 2018-12-12

    Accepted date: 2018-12-12

    Published date: 2018-12-09