Extrusion encapsulation of Lactobacillus bulgaricus coated by carrageenan – alginate with additional tofu waste flour prebiotic

  • Authors

    • Vina Muhardina Department of Agricultural Technology, Faculty of Agricultural Technology, University of Serambi Mekkah
    • Putri Meutia Sari Department of Agricultural Technology, Faculty of Agricultural Technology, University of Serambi Mekkah
    • Yuliani Aisyah Department of Agricultural Product Technology, Faculty of Agriculture, University of Syiah Kuala
    • Sri Haryani Department of Agricultural Product Technology, Faculty of Agriculture, University of Syiah Kuala
    • Fitrah Atul Mega Department of Agricultural Product Technology, Faculty of Agriculture, University of Syiah Kuala
    2019-03-22
    https://doi.org/10.14419/ijet.v7i4.24255
  • Alginate, Carrageenan, Tofu waste flour, Extrusion, Encapsulation, Probiotic.

  • Abstract

    Survival phase of probiotic Lactobacillus bulgaricus, depends on its living conditions, which are processing stages, storage condition and acidity level in digestive tract. The severity states of environment and improper treatment may cause a reduction of probiotic viability in food products. Extrusion method was used to encapsulate the bacteria by using combination of encapsulating agents and a certain percentage of prebiotic in order to enhance the viability of probiotic in acid and cold condition. This study used two factors, which were capsule agents (alginate and carrageenan), and percentages of tofu waste flour (1.5%, 2%, 2.5%, and 3%). The results showed that carrageenan was better in protecting probiotics at pH 2 with a total LAB of 4.23 LogCFU/gram, 42.5% viability of LAB and 41% efficiency encapsulation compared to alginate 3.92 LogCFU/gram, 38.2% viability and 37.9% efficiency encapsulation. The addition of tofu waste flour causes an increase in the growth of probiotic bacteria up to 10.5 LogCFU/gram. In the simulation conditions of gastric acid (pH 2), the combination of the use of carrageenan with the optimal percentage of tofu waste flour (3%) results in the most effective encapsulation conditions for probiotic viability and the highest yield of encapsulation.

     

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  • How to Cite

    Muhardina, V., Meutia Sari, P., Aisyah, Y., Haryani, S., & Atul Mega, F. (2019). Extrusion encapsulation of Lactobacillus bulgaricus coated by carrageenan – alginate with additional tofu waste flour prebiotic. International Journal of Engineering & Technology, 7(4), 5242-5244. https://doi.org/10.14419/ijet.v7i4.24255

    Received date: 2018-12-18

    Accepted date: 2018-12-18

    Published date: 2019-03-22