Identification of Bacterial Community in Wastewater and Their Kinetic Growth in Microbial Fuel Cells

  • Authors

    • S. M. Zain
    • T. A. Naa
    • F. Suja
    • N. A. Zahaimi
    • A. N. Baskharan
    2019-01-30
    https://doi.org/10.14419/ijet.v8i1.2.24880
  • bacterial community, kinetic, microbial fuel cell, wastewater.

  • Abstract

    Microbial fuel cell (MFC) is an alternative energy system comprise of bio-electrochemical reactor with wastewater as raw material and electrochemically active microorganisms as the main biocatalyst that utilize and convert chemical energy within organic matters to electrical energy. Sequence analysis by BLAST indicated that five species existed which were Pseudomonas aeruginosa, Klebsiella pneumonia, Enterobacter aerogenes, Leucobacter luti and Bacillus cereus. Pseudomonas aeruginosa, Klebsiella pneumonia and Enterobacter aerogenes are biofilm and exoelectrogens that responsible to generate electricity in the MFC for both aerated or with the addition of ferricyanide. However, Leucobacter luti and Bacillus cereus are also among the suspended growth bacteria that can be found in the MFC reactor. As for the rate of removal of COD, it showed that with aerated cathode, the COD removal rate was 51.30 % while with the addition of ferricyanide in the cathode showed a COD removal rate of 93 %. The highest voltage generated by the additional of ferricyanide was 0.841 V while for the aerated cathode, the highest voltage of 0.589 V has been generated. As for the kinetic growth of the bacteria, specific maximum growth rate, μnet of the biomass was 0.003 h-1 and 0.004 h-1 for biofilm in the aerated tank while with the addition of ferricyanide, μnet for biomass was 0.002 h-1 and 0.001 h-1 for biofilm. Yield growth, Y for biomass in the aerated tank had the highest growth which was 2.035 g.cell/g.substrate while biofilm only had limited growth of 0.1213 g.cell/g.substrate. As for the addition of ferricyanide, Y for biomass was 1.206 g.cell/g.substrate and 0.3268 g.cell/g.substrate for biofilm.

     


     

  • References

    1. [1] Zain SM, Roslani NS, Hashim AN, Suja F, Daud WRW & Basri, NEA (2011a), Microbial Fuel Cells using Mixed Cultures of Wastewater for Electricity Generation. Sains Malaysiana 40(9): 993-997.

      [2] Zain SM, Hashim R, Roslani NS, Anuar N, Suja F, Daud WRW & Basri NEA (2011b), Pengecaman Awal Komuniti Bakteria Sel Bahan Api Mikrob dalam Air Sisa Kumbahan. Sains Malaysiana 40(9):959-964.

      [3] Syazana

      [4] Zain SM, Ching NL, Jusoh S & Yunus SY (2015), Different Types of Microbial Fuel Cell (MFC) System for Simultaneous Electricity Generation and Pollutant Removal, Jurnal Teknologi 74 (3): 13-19.

      [5] Ismail ZZ & Jaeel AJ (2013), Sustainable Power Generation in Continuous Flow Microbial Fuel Cell Treating Actual Wastewater: Influence of Biocatalyst Type on Electricity Production. The Scientific World Journal 1-7.

      [6] Logan BE, Microbial fuel cells, John Wiley and Sons, New York, (2008).

      [7] Watanabe K, Ishii S, Takefumi S, Yasuaki H (2008), Characterization of a filamentous biofilm community established in a cellulose-fed microbial fuel cell. BMC Microbiology 8(6), 1186-1271.

      [8] Chaudhuri SK, Lovely DR (2003), Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells. Nature Biotechnology 21(10), 1229-1232.

      [9] Kim BH, Kim HJ, Hyun MS, Park DH (1999), Direct electron reaction of Fe (III)-reducing bacterium, Shewanella putrefaciens IR-1. Journal of Microbiology and Biotechnology 9, 127-131.

      [10] Kim HJ, Park HS, Hyun MS, Chang IS, Kim MA, Kim BH (2002), A mediator-less microbial fuel cell using a metal reducing bacterium, Shewanella putrefaciens. Enzyme Microbiol Technology 30, 145-152.

      [11] Rabaey K, Boon N, Siciliano SD, Verhaege M, Verstraete W (2004), Biofuel cells select for microbial consortia that self-mediate electron transfer. Applied and Environmental Microbiology 70(9), 5373-5382.

      [12] Bond DR, Holmes DE, Tender LM, Lovley DR (2002), Electrode-reducing microorganisms that harvest energy from marine sediments. Science 295, 483-485.

      [13] Bond DR, Lovley DR (2003), Electricity production by Geobacter sulfurreducens attached to electrodes. Applied and Environmental Microbiology 69(3), 1548-1555.

      [14] Park HS, Kim BH, Kim HS, Kim HJ, Kim GT, Kim M, Chang IS, Park YK, Chang HI (2001), A novel electrochemically active and Fe (III) reducing bacterium phylogenetically related to Clostridium butyricum isolated from a bacterial fuel cell. Anaerobe 7: 297-306.

      [15] Yi Z, Defeng X, John MR, Logan BE (2009), Isolation of the exoelectrogenic bacterium Ochrobactrum anthropi YZ-1 by using a U-tube microbial fuel cell. Applied and Environmental Microbiology 74(10), 3130–3137.

      [16] Kim JR, Jung SH, Regan JM, Logan BE (2007), Electricity generation and microbial community analysis of ethanol powered microbial fuel cells. Bioresource Technology 41(3), 1004-1009.

      [17] Oh S & Logan BE (2006), Proton exchange membrane and electrode surface areas as factors that affect power generation in microbial fuel cells. Appl. Microbiol. Biotechnol. 70(2), 162-169.

      [18] Ringeisen BR, Henderson E, Wu PK, Pietron J, Ray R, Little B, Biffinger JC, Jones-Meehan JM (2006), High power density from a miniature microbial fuel cell using Shewanella oneidensis DSP10. Enviromental Science and Technology 40(8), 2629-2634.

      [19] Kiely PD, Call DF, Yates MD, Regan JM, Logan BE (2010), Anodic biofilms in microbial fuel cells harbor low numbers of higher power producing bacteria than abundant genera. Applied Microbiology and Biotechnology 88(1), 371–380.

      [20] Richter H, Nevin KP, Jia H, Lowy DA, Lovley DR, Tender LM (2009), Cyclic voltammetry of biofilms of wild type and mutant Geobacter sulfurreducens on fuel cell anodes indicates possible roles of OmcB, OmcZ, type IV pili, and protons in extracellular electron transfer. Energy and Environmental Science 2, 506-516.

      [21] Min B, Kim JR, Oh S, Regan JM, Logan BE (2005), Electricity generation from swine wastewater using microbial fuel cell. Water Research 39(20), 4961-4968.

      [22] NSN Hisham, SM Zain, S Jusoh, N. Anuar, F. Suja, A. Ismail, NEA Basri (2013), Microbial Fuel Cells Using Different Types of Wastewater for Electricity Generation and Simultaneously Removed Pollutant. Journal of Engineering Science and Technology 8(3):317-326.

      [23] Ishii S, Suzuki S, Norden-Krichmar, TM, Wu A, Yamanaka Y, Nealson KH & Bretschger O (2013), Identifying the microbial communities and operational conditions for optimized wastewater treatment in microbial fuel cells. Water Research 47(19): 7120-7130.

      [24] MZM, Yusoff A. Hu C, Feng T, Maeda Y, Shirai MA, Hassan & Yu CP (2013), Influenced of pretreated activated sludge for electricity generation in microbial fuel cell application. Bioresource Technology 145: 90-96.

      [25] Tunc C, Li K, Hakan B, Liu H (2008), Electricity production from twelve monosaccharide using microbial fuel cells. Journal of Power Sources 175,196–200.

      [26] Deng LF, Li FB, Zhou SG, Huang DY, Ni JR (2010), A study of electron-shutle mechanism in Klebsiella pneumonia based-microbial fuel cells. Envr. Sci &Tech 55, 99-104.

      [27] Zhuang L, Zhou S, Yuan Y, Liu T, Wu Z & Cheng L (2010), Development of Enterobacter aerogenes fuel cells: From in situ biohydrogen oxidation to direct electroactive biofilm. Bioresource Technology 102(1): 284-289.

      [28] Mohan SV, Saravanan R, Raghavulu SV, Mohanakrishna G, Sarma, PN (2008a), Bioelectricity production from wastewater treatment in dual chambered microbial fuel cell (MFC) using selectively enriched mixed microflora: Effect of catholyte. Bioresource Technology 99, 596-603.

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  • How to Cite

    M. Zain, S., A. Naa, T., Suja, F., A. Zahaimi, N., & N. Baskharan, A. (2019). Identification of Bacterial Community in Wastewater and Their Kinetic Growth in Microbial Fuel Cells. International Journal of Engineering & Technology, 8(1.2), 103-108. https://doi.org/10.14419/ijet.v8i1.2.24880

    Received date: 2018-12-28

    Accepted date: 2018-12-28

    Published date: 2019-01-30