Fermentation Optimization on Bacterial Cellulose Production by Acetobacter Xylinum 0416

 
 
 
  • Abstract
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  • Abstract


    Bacterial cellulose (BC) has received extensive attention in various field due to of its high purity, mechanical strength, crystallinity and biodegradability. Determination of optimum fermentation condition for the improvement of cellulose production is essential especially in industrial scale. The production of bacterial cellulose by A.xylinum 0416 was fermented in matured coconut water medium and the suitable culture conditions were predicted statistically via Central Composite Design of Response Surface Methodology. Effect of varying temperature, fermentation period, initial pH, inoculum size and parameters interaction were studied in five-levels Design of Experimental approach. Temperature, inoculum size and fermentation period had significant effects on the cellulose production. By aiming to minimize the temperature and fermentation period for maximum cellulose production, the optimized parameters predicted for cellulose production of 1.12829 g dry weight were temperature: 27˚C, fermentation period: 5 days, initial pH: pH 8 and inoculum size: 15% with high desirability of 78.2%.

     

     


  • Keywords


    Acetobacter xylinum 0416; bacterial cellulose; central composite design; fermentation; optimum parameter.

  • References


      [1] Sengun IY & Karabiyikli S (2011). Importance of acetic acid bacteria in food industry. Food Control 5, 647-656.

      [2] Rivas B, Moldes AB, Domínguez JM & Parajó JC (2004). Development of Culture Media Containing Spent Yeast Cells ofdebaryomyces Hansenii and Corn Steep Liquor for Lactic Acid Production with Lactobacillus Rhamnosus. International Journal of Food Microbiology 97(1), 93–98.

      [3] Bhaumik R, Mondal NK, Chattoraj S & Datta JK (2013), Application of response surface methodology for optimization of fluoride removal mechanism by newely developed biomaterial. American Journal of Analytical Chemistry 4, 404–419.

      [4] Norouzian D, Farhangi A, Tolooei S, Saffari Z, Mehrabi MR, Chiani M, Ghassemi S, Farahnak M & Akbarzadeh A (2011). Study of Nano-fiber Cellulose Production by Glucanacetobacter xylinum ATCC 10245. Pakistan Journal of Biological Sciences 14, 780-784.

      [5] Bilgi E, Bayir E, Sendemir-Urkmez A, Hames EE (2016). Optimization of bacterial cellulose production by Gluconacetobacter xylinus using carob and haricot bean. Int J Biol Macromol 90, 2-10.

      [6] Chawla PR, Bajaj IB, Survase SA & Singhal RS (2009). Fermentative production of microbial cellulose. Food Technol. Biotechnol 47(2), 107–124.

      [7] Norliza, A. R., Azmi, M., Atif, M., Zainuri, A., Izzuddin, M., Lupang, L. S., Norasila, K., Jaylakshumi, G. & Faezah, E. (2015). Application of Process Control in Production of Biocellulose : A Case Study 755, 960–963.

      [8] Harun, S., V. Balan, M. S. Takriff, O. Hassan, J. Jahim & B. E. Dale (2013). Performance of AFEX™ pretreated rice straw as source of fermentable sugars: the influence of particle size. Biotechnology for Biofuels 6(40): 1 - 17


 

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Article ID: 24912
 
DOI: 10.14419/ijet.v8i1.2.24912




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