Inhibitory Effect of 5-Aza-2’-Deoxytidine on Cell Viability of the Oral Cancer Cell Line, ORL-48T

  • Authors

    • N. M.S Rawaidah
    • N. S Syairah
    • G. H Khor
    • I. M. Amin
    • N. Harun
    • M. Y.P.M. Yusof
    • G. R.A Froemming
    2018-12-29
    https://doi.org/10.14419/ijet.v7i4.42.25699
  • Oral Cancer, 5-aza-2'-deoxycytidine, MTT Assay, Cell Viability.

  • Abstract

    Oral cancer is recorded as the sixth highest malignancy globally. 5-Aza-2'-deoxycytidine (5-Aza-dC) has been applied as an inhibitory agent in various tumourous cells. Previous studies showed that 5-Aza-dC can inverse genes silencing resulting from hypermethylation process. Inhibitory effects of 5-Aza-dC on oral cancer cell lines, ORL-48T proliferation was studied using 3-(4,5-dimethylthiazol-2-yl)-2,5 -diphenyltetrazolium bromide (MTT)-based assay in this study. The results demonstrated the treatment with 20 µM 5-Aza-dC for 144 h and 40 µM with 72 h respectively, revealed a significantly decreased percentage of viable tumour cells (p= 0.03). This current study provides an evidence of the inhibitive effect of 5-Aza-dC on the ORL-48T cells proliferation.

     

     

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  • How to Cite

    M.S Rawaidah, N., S Syairah, N., H Khor, G., M. Amin, I., Harun, N., Y.P.M. Yusof, M., & R.A Froemming, G. (2018). Inhibitory Effect of 5-Aza-2’-Deoxytidine on Cell Viability of the Oral Cancer Cell Line, ORL-48T. International Journal of Engineering & Technology, 7(4.42), 145-147. https://doi.org/10.14419/ijet.v7i4.42.25699

    Received date: 2019-01-11

    Accepted date: 2019-01-11

    Published date: 2018-12-29