Callus Induction from Leaf Explants of Mas Cotek (Ficus Deltoidea var. bilobata) Treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram)

  • Authors

    • Azrina Ismail
    • Norshazila Norshazila
    • Rashidi Othman
    • Nashriyah Mat
    2018-12-29
    https://doi.org/10.14419/ijet.v7i4.43.25841
  • Callus, Ficus deltiodea var bilobata, induction, 6-Benzyl Amino Purine (BAP), 4-amino-3, 5, 6-trichloro picolinic acid (Picloram).

  • Abstract

    The objective of this study was to observe the effect of 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-trichloropicolinic acid (Picloram) on callus induction of Mas Cotek (Ficus deltoidea var bilobata) explant. Murashige and Skoog (MS) nutrient media supplemented with 6-Benzylaminopurine (BAP) and 4-amino-3,5,6-trichloro picolinic acid (Picloram) each at different concentrations of 0 mg/l, 0.5 mg/l , 1.5 mg/l and 3.0 mg/l, respectively. The control and other six treatments gave negative responses to the MS media supplemented with various concentrations of BAP and Picloram. Most of the callus began to form at day 16 up to day 30 with treatment.  Hard and yellowish callus with moderate growth rate was obtained from explants cultured on MS medium supplemented with 0.5 mg/ l BAP and 1.5 mg/l Picloram, wherein the callus formation was recorded at 47 %. Hard and greenish callus was obtained from medium treated with 0.5 mg/l BAP and 0.5 mg/l Picloram after 30 days of treatment with callus formation of 26.67 %. Medium supplemented with 0.5 mg/l of BAP hormone and 1.5 mg/l of Picloram hormone displayed the best growth rate of callus and was the most suitable medium for callus induction.

     

     

  • References

    1. [1] Ghazali, M.M. (2010) Mas Cotek: Tumbuhan Herba Ajaib. Retrieved 13th May 2010 from http://www.majalahsains.com/2010/11/mas-cotek-tumbuhan-herba-ajaib/

      [2] Berg, C.C. & Corner, E.J.H. (2005) Flora Malaysiana. Series I, Vol 17/Part 2. National Herbarium Nederland. The Netherlands: Universiteit Leiden branch.

      [3] Berg, C.C. & Corner, E.J.H.(2005) Moraceae (Ficus). Series 1- Seed Plants. Flora Malesiana 17 (2), 1-729.

      [4] Hamed, M.A. (2011) Beneficial effect of Ficus Linn. On high fat-induced hypercholesterolemia in rats. Food Chemistry 129, 162-170.

      [5] Starr, F., Starr, K., Loope, L. (2003) Ficus Deltoidea. Retrieved on 9th February 2009 from http://www.hear.org/starr/hiplants/report/html/ficus_deltoidea.htm.

      [6] Nostro, A., Germano, M.O., D’Angelo, V., Marino, A. & Cannatelli, M.A. (2000) Extraction method and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology 30: 37-384.

      [7] Yvonne, L.S.Y. (2003) Herbs for verve. Retrieved from http://thestar.com.my/lifestyle/story.asp?file=/2003/7/15/features/5735054&sec=features on (2005, August 16).

      [8] Osman, D. (2004) Menggali khazanah alam emas cotek. Retrieved from http://delto.my.com/karangkraf.htm on (29 December, 2017).

      [9] Zaki, W.W.M. (2005) Dry matter partitions and yield potential of Mas Cotek (Ficus deltoidea) grown on bris soil. Seminar on Medicinal and Aromatic Plants, 2005, September 13-14, pp: 39.

      [10] Zaidah, R., Aizi Nor Mazila R., Izza, A.A., Liew, W.,M., Noor Afida, A.F., Nor Fadzilah, M.Z., Nor Kamila, K., Pua, S.S., Azrul Razi, M., Siti Marziah, M.D. & Zahirah, Z. (2006) Genomic extraction of several Malaysian herbal plants. Proceedings Annual 2006, p. 177-183. Johor Bahru: Ibnu Sina Institute for Fundamental Science Studies, UTM.

      [11] Gamborg, O.L., Murashige, T., Thorpe, T.A. & Vasil, I.K. (1976) Plant Tissue Culture Media. In Vitro 12, 473-478.

      [12] Drossard, J., Nahring, J.M., Hellwig, S. & Fisher, R. (1997) Production of engineered antibodies in tobacco plants and cell suspension cultures. In Antibody Engineering, New Technology, Application and Commercialization, vol. 2, IBC’s 8th annual international conference on antibody engineering, p:69-93 (IBC Library Services, Southborough, Massachusetts, 1997).

      [13] Fischer, R., Liao, Y.C. & Drossard, J. (1999) Affinity-purification of a TMV-specific recombinant full size antibody from a transgenic tobacco suspension culture. Journal of Immunology Methods 226, 1-10.

      [14] Shahidan, N., Othman, R., Jaswir, I., Hasyun-Hashim, Y. Z. (2017) Effect of Abiotic Stress under Light and Dark Conditions on Carotenoid Content in Pumpkin (Cucurbita moshata) Calluses. Journal of Fundamental and Applied Science 9(2S), 861-875.

      [15] Dodds, J.H. & Roberts LW. (1995) Experiments in Plant Tissue Culture. 3rd edition , Cambridge University Press, Australia, p: 248.

      [16] Kedra, M. & Bach, A. (2005) Morphogenesis og Lilium martagon L. explants in callus culture. Acta Biologica Cracoviensia Series Botanica 47, 65-73.

      [17] Beyl, C.A. & Sharma, G.C. (1983) Picloram induced somatic embryogenesis in Gasteria and Haworthia. Plant Cell, Tissue and Organ Culture 2, 123–132.

      [18] Sellés, M., Viladomat, J., Bastida, J. & Codina, C. (1999) Callus induction, somatic embryogenesis and organogenesis in Narcissus confusus: correlation between the state of differentiation and the content of galanthamine and related alkaloids. Plant Cell Reports 18, 646–651.

      [19] Figueiredo, S.F.L., V.R.C.Vuana, C. Simoes, L.C. Trugo & M.A.C. Kaplan. (2003) Rolllinia mucosa (Jacq) Baill.: Establishment of callus culture and lignan production. Revista Cubana de Plantas Medicinales, p: 8.

      [20] Fujimura, T. & Komamine, A. (1975) Effects of various growth regulators on the embryogenesis in a carrot cell suspension culture. Plant Science Letters 5(6), 359-364.

      [21] Grossman, K., Kwiatkowski, J. & Tresch, S. (2001) Auxin herbicides induce H2O2 overproduction and tissue damage in cleavers (Galium aparine L.). Journal of Experimental Botany 52 (362), 1811–1816.

      [22] George, E.F. & Sherrington, P.D. (1984) Plant propagation by tissue culture. Exegetics Ltd., Eversley, Basingstoke, 39-71.

      [23] Franklin, C.I. & Dixon, R.A. (1994) Initiation and Maintenance of Callus and Cell Suspension Cultures, In: Dixon, R.A. and R.A. Gonzales (Eds), Plant Cell Culture: A practical Approach 2nd Edn, Irl Press, United States.

      [24] Mohan, R.Y. & Saritha, K.V. (2012) Callus induction and somatic embryogenesis of Gardenia latifolia Ait. International Jurnal of Current Science 4, 83-89.

      [25] Dodds, J.H. & Roberts, L.W. (1995) Experiments in Plant Tissue Culture. 3rd edition , Cambridge University Press, Australia, p, 248.

      [26] Sivaram, L. & Mukundan, U. (2003) In vitro culture studies on Stevia rebaudian. In Vitro Cellular & Developmental Biology, Plant, 39, 520-523.

      [27] Rangappa K. & Aind DS. (2013) High frequency multiplication of shoots using axillary buds for production of elite lines of Stevia rebaudiana. Advances in Bioscience and Biotechnology 4, 781-784.

      [28] Rashidi O. (2009) Biochemistry and genetics of carotenoid composition in potato tubers. PhD thesis. Lincoln University.

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  • How to Cite

    Ismail, A., Norshazila, N., Othman, R., & Mat, N. (2018). Callus Induction from Leaf Explants of Mas Cotek (Ficus Deltoidea var. bilobata) Treated with 6-Benzylaminopurine (BAP) and 4-Amino-3, 5, 6-Trichloropicolinic acid (Picloram). International Journal of Engineering & Technology, 7(4.43), 164-167. https://doi.org/10.14419/ijet.v7i4.43.25841

    Received date: 2019-01-13

    Accepted date: 2019-01-13

    Published date: 2018-12-29