Separation of Biological Molecules using Electrochemically Etched Nanoporous Silicon Membrane
Keywords:Nanoporous silicon membrane, PDMS, electrochemical etching, biological molecules, indium oxide
This paper presents a separating technique of biological molecules and non-biological particles in solution using nanoporous silicon membranes. The simple separation method has been studied by fabricating the porous silicon membrane with Polydimethylsiloxane (PDMS) to produce the filtration system. The testing procedure began with diluted and sonicated indium oxide particles with deionized (DI) water and mixed with a biological solution. In this experiment setup, biological particles used is protein standard, serum albumin and sodium chloride has been filtered out through this filtration system. As a result, the indium oxide particles were trapped on the nanoporous silicon surface. Meanwhile, biology molecules penetrate the nanoporous silicon membrane with particle and molecule sizes up to 15 nm. The filtered-out particles are inspected under Zetasizer Nano SP to count and measure the size of the particles and molecules. According to the inspected result show 98% biological molecules are filtered out from this nanoporous silicon membrane. Due to this simple fabrication process, porous silicon membranes are able to be integrated to the other component to develop the complete bioMEMS and Lab on Chip system in the future.
style='mso-spacerun:yes'> ADDIN EN.REFLIST
field-separator'> S. Wickramasinghe, B. Kalbfuss, A. Zimmermann, V. Thom, and U. Reichl, "Tangential flow microfiltration and ultrafiltration for human influenza A virus concentration and purification," Biotechnology and bioengineering, vol. 92, pp. 199-208, 2005.
 W. H. Fissell, A. Dubnishev, A. N. Eldridgeb, A. J. Fleischman, A. L. Zydneyc, and S. Roy, "High-performance silicon nanopore hemofiltration membranes," Journal of Membrane Science, vol. 326, pp. 58-63, 2009.
 A. Hamzah, H. Z. Abidin, B. Y. Majlis, M. M. Nor, A. Ismardi, G. Sugandi, et al., "Electrochemically deposited and etched membranes with precisely sized micropores for biological fluids microfiltration," Journal of Micromechanics and Microengineering, vol. 23, p. 074007, 2013.
 J. Alvankarian and B. Y. Majlis, "A new UV-curing elastomeric substrate for rapid prototyping of microfluidic devices," Journal of Micromechanics and Microengineering, vol. 22, p. 035006, 2012.
 M. Masrie, B. Y. Majlis, and J. Yunas, "Fabrication of multilayer-PDMS based microfluidic device for bio-particles concentration detection," Bio-medical materials and engineering, vol. 24, pp. 1951-1958, 2014.
 R. Rupasinghe, "Dissolution and aggregation of zinc oxide nanoparticles at circumneutral pH; a study of size effects in the presence and absence of citric acid," 2011.
 P. Kumar, P. Lemmens, M. Ghosh, F. Ludwig, and M. Schilling, "Effect of HF Concentration on Physical and Electronic Properties of Electrochemically Formed Nanoporous Silicon," Journal of Nanomaterials, vol. 7, pp. 1-7, 2009.
 M. J. Sailor, "Fundamentals of Porous Silicon Preparation," in Porous Silicon in Practice: Preperation, Characterization and Applications, ed: Wiley-VCH Verlag GmbH & Co. KGaA, 2012, pp. 1-42.
 J. Salonen and V. P. Lehto, "Fabrication and chemical surface modification of mesoporous silicon for biomedical applications," Chemical engineering journal, vol. 137, pp. 162-172, 2008.
 X. G. Zhang, "Morphology and formation mechanisms of porous silicon," Journal of the Electrochemical Society, vol. 151, pp. 69-80, 2004.
 G. J. Tortora and B. Derrickson, Principles of Anatomy and Physiology, 11 ed.: John Wiley & Sons Inc, 2006.
 N. Burham, A. A. Hamzah, and B. Y. Majlis, "Self-Adjusting Electrochemical Etching Technique for Producing Nanoporous Silicon Membrane," in New Research on Silicon-Structure, Properties, Technology, ed: InTech, 2017.
 T. Urata, K. Fukami, T. Sakka, and Y. H. Ogata, "Pore formation in p-type silicon in solutions containing different types of alcohol," Nanoscale research letters, vol. 7, p. 329, 2012.
 Ã‰. VÃ¡zsonyi, E. SzilÃ¡gyi, P. Petrik, Z. E. HorvÃ¡th, T. Lohner, M. Fried, et al., "Porous silicon formation by stain etching," Thin Solid Films, vol. 388, pp. 295-302, 6/1/ 2001.
 S. Yaakob, M. A. B. Ismail, N. H. H. A. Bakar, and K. Ibrahim, "The formation and morphology of highly doped N-type porous silicon : Effect of short etching time at high current density and evidence of simultaneous chemical and electrochemical dissolutions," Journal of Physical Science, vol. 23, pp. 17-31, 2012.