Characterization of active compounds from Jatropha gossypifolia L. based on HPTLC

  • Authors

    • Siti Rofida
    • Nailis Nailis
    • Nurkhasanah .
    • Laela Hayu Nurani
    2019-01-26
    https://doi.org/10.14419/ijet.v8i1.9.26384
  • Fingerprint, active compounds, anticancer, ethanolic extract of Jatropha gossypifolia L.

  • Jatropha gossypifolia L. is one of plants that come from Euphorbiaceae family.That is used to be a medicine, particularly as an anticancer. The previous research has reported that the root extract of J.gossypifolia L. (EEAJG) has an ability to kill the cervix cancer cells and breast cancer cells. The cytotoxicity of EEAJG on breast cancer cell lines (T47D) and cervix cancer cell lines (Hella) respectively contained by 43.57 mg/mL and 4.32 mg/mL. The consistency of active compouds on traditional medicine can assurance the biological activity of medicinal plants. Fingerprint profiles of active compouds is either one that can be used to indicate the composition of secondary metabolites in medicinal plants. In this study aims to obtain a fingerprint profiles of active compouds in EEAJG to ensure biological activity as an anticancer based on HPTLC.

    The root extract of J.gossypifolia L. obtained from extraction by maceration with ethanol. Fingerprint profiles of active compouds can be identified by using High Performance Thin Layer Chromatography (HPLC). Secondary metabolites of EEAJG separated by TLC techniques and scaned wavelength of 254 nm and 366 nm with CAMAG TLC scanner 3. The chromatogram were recorded. The results showed that the chemical compounds were detected at 254 nm produced ten peaks with maximum absorbance at 250-350 nm region. The chemical compounds that were detected at 366 nm produced six peaks with absorbance maximum at 200-350 nm region.

     

     

  • References

    1. [1] Bandaranayake, W.M., (2006). Quality Control, Screening, Toxicity and Regulation of Herbal Drugs. In: Ahmad, I., Aqil, F., Owais, M. (Eds). Modern Phytomedicine Turning Medicinal Plant into Drugs. Weinheim: Wiley-VCH VerlagGmbH&Co. KGaA.

      [2] Bauer,R., (1998). Quality Criteria and Standardization of Phytopharmaceuticals: Can Acceptable Drug Standards Be Achieved?. Drug Information Journal, 32:101-110.

      [3] BPOM, (2004). Ketentuan Pokok Pengelompokan dan Penandaan Obat Bahan Alam Indonesia. Jakarta.

      [4] Busse,W., (2000). The Significance of Quality for Efficacy and Safety of Herbal Medicinal Products.Drug Information Journal, 34: 15-23.

      [5] Debenedetti,S.L., (2009). TLC and PC. In: Sampietro,D.A., Catalan,C.N., Vattuone,M.A.,Narwal, S.S. (Eds). Isolation, Identification and Characterization of Allelochemicals/Natural products. (pp.103-134). New Hampshire: Science Publishers, Enfield.

      [6] Falodun,A.,Sheng-Xiang,Q., Parkinson,G., Gibbons,S., (2012). Isolation and Characterization of a new anticancer diterpenoid from Jatropha gossypifolia. Pharmaceutical Chemistry Journal, 10(45), 636-639.

      [7] Gbeassor, M., Kossou, Y., Amegbo, K., De Souza, C., Koumaglo, K., Denke, A., (1989). Antimalarial effects of eight African medicinal plants. Journal of Ethnophamacology 25, 115–118.

      [8] Hutpea, J.R., (1994). Inventaris Tanaman Obat Indonesia (III). (pp.113). Jakarta: Badan Penelitian dan Pengembangan Kesehatan Departemen Kesehatan Republik Indonesia,

      [9] Jansen, O., Angenot, L., Tits, M., Nicolas, J.P., De Mol, P., Nikie’ma, J.-B., Fre’de’rich, M., (2010). Evaluation of 13 selected medicinal plants from Burkina Faso for their antiplasmodial properties. Journal of Ethnopharmacology 130, 143–150.

      [10] Kupchan, S.M., Sigel, C.W., Matz, M.J., Bryan, R.F., (1970). Jatrophone, a novel macrocyclic diterpenoid tumor inhibitor from Jatropha gossypiifolia. Journal of American Chemical Society 92 (14), 4476.

      [11] Mabry,T.J., Markham,K.R., Thomas,M.B., (1970). The Systematic Identification of Flavonoids. (pp.12-15). Berlin: Springer-Verlag..

      [12] Oduola, T., Avwioro, O.G., Ayanniyi, T.B., (2005). Suitability of the leaf extract of Jatropha gossypiifolia as an anticoagulant for biochemical and haematological analyses. African Jounal of Biotechnology 4 (7), 679–681.

      [13] Ogundare, A.O., (2007). Antimicrobial effect of Tithonia Diversifolia and Jatropha gossypiifolia leaf extracts. Trends In Applied Sciences Research 2 (2), 145–150.

      [14] Panda, B.B., Gaur, K., Kori, M.L., Tyagi, L.K., Nema, R.K., Sharma, C.S., Jain, A.K., (2009). Anti-inflammatory and analgesic ativity of Jatropha gossypiifolia in experimental animal models. Global Journal of Pharmacology 3 (1), 1–5.

      [15] Panda, B.B., Gaur, R.K., Sharma, C.S., Jain, A.K., Jain, C.P., (2009). Hepatoprotective activity of Jatropha gossypiifolia against carbon tetrachloride-induced hepatic injury in rats. Asian Journal of Pharmaceutical and, Clinical Research 2.

      [16] Rajani, M., Kumar, V., Chauhan, N., Padh, H., (2006). Search for antibacterial and antifungal agents from selected Indian medicinal plants. Journal of Ethnopharmacology 107, 182–188.

      [17] Rofida, S.,Syifa,N.,Nurkhasanah, Nurani,H.L., (2016). Anticancer Activity of Jatropha sp. on Breast Cancer Cells and Cervix Cells. Proceeding International Seminar TOI, ISBN : 978-602-72418-2-8, 102-107.

      [18] Singh, D., Singh, A., (2002). Biochemical alteration in freshwater fish Channa punctatus due to lattices of Euphorbia royleana and Jatropha gossypiifolia. Environmental Toxicology and Pharmacology 12, 129–136.

      [19] Spangenberg,B., (2008). Derivatization, Detection (Quantification), and Identification of Compounds Online. In: Waksmundzka-Hajnos,M.,Sherma,J.,Kowalska,T. (Eds). Thin Layer Chromatography in Phytochemistry. (pp. 175-189). New York: CRC Press,

      [20] Srivastava,M.M., (2011). An Overview of HPTLC: A Modern Anlytical Technique with Excellent Potential for Automation, Optimization, Hyphenation, and Multidimensional Applications. In: Srivastava,M.M. (Eds). High-Performance Thin Layer Chromatography (HPTLC). (pp.3-21). Berlin: Springer-Verlag.

      [21] Valencia, A., Frérot, B., Guénego, H., Múnera, D.F., Grossi De Sá, M.F., Calatayud, P.A., (2006). Effect of Jatropha gossypiifolia leaf extracts on three lepidoptera species. Revista Colombiana de Entomología 32 (1), 45–48.

      [22] World Health Organization, (2005). Good Manufacturing Practices: Updates Supplementary Guidelines for The Manufacture of Herbal Medicinal Products. Geneva

      [23] World Health Organization, (2011), Traditional, Complementary and Herbal Medicine. Retrieved from http://apps.who.int/medicinedocs /en/d/Jh2996e/6.2.html.

      [24] Yadav, N.P., Dixit, V.K., (2008). Recent approaches in herbal drug standardization. International Journal of Integrative Biology, 3(2):195-203.

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    Rofida, S., Nailis, N., ., N., & Hayu Nurani, L. (2019). Characterization of active compounds from Jatropha gossypifolia L. based on HPTLC. International Journal of Engineering & Technology, 8(1.9), 127-130. https://doi.org/10.14419/ijet.v8i1.9.26384