The Expression of Drought Resistance GmDREB-1 Gene on Soybean (Glycine max L. Merr) as Result of EMS Mutation Induction (Etyl Methane Sulfonate)

  • Authors

    • Evika Sandi Savitri
    • Ruri Siti Resmisari
    2019-01-26
    https://doi.org/10.14419/ijet.v8i1.9.26409
  • GmDREB-1, Mutation Induction, EMS

  • Abstract

    Abiotic stresses, particularly drought, are harmful for the plant productivity. Mutagenesis is widely used as method that is potential to increase the variability of crop development. Mutagenic using EMS (Ethyl Methane Sulfonate) shows more effective results than using other mutagens. DREB1 gene is a subclass of DREB gene which is a transcription factor and it also serves as a key regulatory response of plants to drought stress. Mutation induction combines concentration treatment and immersion time in EMS.  RNA isolation employs RNeasy Plant Mini Kit (Qiagen). GmDREB-1 expression is analyzed using Real Time PCR (Quantitative Real-time Polymerase Chain Reaction or Q-PCR). The analysis shows that mutation treatment with EMS using 0.07% concentration with a wide range of immersion time indicates that GmDREB-1 expression is higher than other treatments.  The results of this study proved that mutation induction using EMS can be used as an alternative to plant breeding to obtain drought resistant soybean.  Induction mutation useful for improvement genetic variability of plant.

     

     

  • References

    1. [1] Atman Dan Hosen, 2008. Dukungan Teknologi Dan Kebijakan Dalam Pengembangan Tanaman Kedelai Di Sumatera Barat. Scientific Journal Tambua. VII (3), September-December 2008: 347-359

      [2] Farrell RE. 2005. RNA methodologies: A laboratory Guide for Isolation and Characterization. 3rd ed. Elsevier Academic Press, Burlington

      [3] Girija SP and Apparao J. 2011. Studies on Effectiveness and Efficiency of EMS in Pigeonpea (Cajanus cajan L.). Bioscience Discovery, 2: 29-31.

      [4] Hoffmann. 2009. A Review of RT-PCR Technologies Used in Veterinary Virology and Disease Control: Sensitive and Specific Diagnosis of Five Livestock Diseases Notifiable to the World Organisation for Animal Health. Available from journal homepage: www.elsevier.com/locat e/vetmic

      [5] Ingram J, Bartels D. 1996. The Molecular Basis of Dehydration Tolerance in Plants. AMU Rev Plant Physiol Plant Mo1 Biol 47:377403

      [6] Kaul, M.L.H. and Bhan, A. K. 1977. Mutagenic Effectivenes and Efficiency of EMS, DES, and Gamma-Rays in Rice. Theoretical and Applied Genetics. 50:241-246

      [7] Khan and Tyagi, 2010. Studies on Effectiveness and Efficiency of Gamma Rays, EMS and Their Combination in Soybean (Glycine Max L. Merril). Journal of Plant Breeding and Crop Science 2(3) : 55-58

      [8] Kubista.M Jose´ Manuel Andrade; Martin Bengtsson a,c, Amin Forootan d, Jiri Jona´k. 2006. The Real-Time Polymerase Chain Reaction. Molecular Aspects of Medicine 27: 95–125.

      [9] Micke, A., M. Maluszynski, and B. Donini 1985. Plant Cultivars Derived From Mutation Induction or the Use of Induced Mutants in Cross Breeding. Mutation Breeding Review 3. IAEA, Vienna

      [10] Rahman SM., Takagi, Y., Kubota, K., Miyamoto K., and Kawakita T. 1994. High Oleic Acid Mutant in Soybean Induced By X-Ray Irradiation. Biosci. Biotech. Biochem. 58 : 1070-1072

      [11] Rapley R, Heptinstall J. 1998. Uv Spectrophotometric Analysis of Ribonucleic Acids. In: Rapley R, Manning DL (Eds.). 1998. Methods in molecular biology. (86). RNA: Isolation and characterization protocols. Humana Press Inc., Totowa

      [12] Shah TM, Mirza JI, Haq MA and Atta BM. 2008. Induced Genetic Variability in Chickpea (Cicer Arietinum L.). II. Comparative Mutagenic Effectiveness and Efficiency of Physical and Chemical Mutagens. Pak. J. Bot., 40(2): 605-613

      [13] Shinozaki, K., & KY. Shinozaki. 1997. Gene Expression and Signal Transduction in Water Stress Response. Plant Physiology 115 : 327 – 334

      [14] Singh, S.P. 2001. Breeding The Genetic Base of Common Bean Cultivars: A Review. Crop Sci. 41:1659–1675

      [15] Solanki and Sharma, 1994. Gamma Rays, Ethyl Imine and N-Nitroso-N-Ethyl Urea in Macrosperma Lentil (Lens Culinaris Medik). Ind. J. Genet. 4 : 72-76

      [16] Subandi. 2007. Tenologi Produksi dan Strategi Pengembangan Kedelai Pada Lahan Kering Msam. http://www.puslittan.bogor.net (February 12, 2007).

      [17] Subuthi, P.K., B.K. Mohapatra and S.K. Sinha 1991. Use of Pollen Traits for Early Detection of Induced Micro Mutations in Wheat. Ind. J. Genet. 51:101- 111.

      [18] Thilagavathi and Mullainathan, 2009. Isolation of Macro Mutants and Mutagenic Effectiveness, Efficiency in Blackgram (Vigna Mungo (L). Hepper). Global J.Mol.Sci. 4: 76-79

      [19] Yamaguchi-Shinozaki K, Shinozaki K. 1994. A Novel Cis-Acting Element in an Arabidopsis Gene is Involved in Responsiveness to Drought, Low-Temperature, or High-Salt Stress. Plant Cell6 251-264

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  • How to Cite

    Sandi Savitri, E., & Siti Resmisari, R. (2019). The Expression of Drought Resistance GmDREB-1 Gene on Soybean (Glycine max L. Merr) as Result of EMS Mutation Induction (Etyl Methane Sulfonate). International Journal of Engineering & Technology, 8(1.9), 250-253. https://doi.org/10.14419/ijet.v8i1.9.26409

    Received date: 2019-01-22

    Accepted date: 2019-01-22

    Published date: 2019-01-26