Antibacterial Activities Test of the Xanthorrhizol Bioactive Compound in the Endophytic Bacteria of Curcuma xanthorrhiza

  • Authors

    • Ulfah Utami
    • Choirul Fuadati
    • C. Ida Alfiah
    https://doi.org/10.14419/ijet.v8i1.9.26670
  • xanthorrhizol, docking, endophytic bacteria, antibacterial

  • Abstract

    The objective of this study is to understand the potential of xanthorrhizol bioactive compound of the endophytic bacteria of Curcuma xanthorrhiza rhizome as an antibacteria  in vitro and the molecular docking simulation. The potency of two endophytic bacteria isolates (ET 1 and ET 2) as the antibacterial producer was tested, then the xanthorrhizol compound was separated using the Thin Layer Chromatography (TLC) and the contact-autobiography TLC.  Furthermore, a docking simulation analysis of the xanthrorrhizol compound as an antibacteria was made. The endophytic bacteria isolate ET1 shows its antibacterial activities to Escherichia coli (6.40 mm)  and Staphylococcus aureus (21.48 mm with the inhibiting power  under category of very strong), while the endophitic bacteria isolate  ET2 shows its antibacterial activities to E. coli (4.52 mm) and S. ureus (8.56 mm). The results of the bioautography -TLC test of the bioactive compound of the  endophitic bacteriais isolate ET1 (Rf 0.57) and isolate ET2 (Rf 0.57)  the visualization of 254 UV lamp is assumed to be the xanthorrizol compound, does not show  antibacterial activities in E. coli and S. aureus. Based on docking simulation results can be seen that xanthorrhizol compounds have the potential to interact with the target protein in  E. coli and S. aureus, but it   is lower than the control antibiotic.

     

     

  • References

    1. [1] Adila, R., Nurmiati, dan Anthoni, A. 2013. Antimicrobial Test Curcuma spp. on growth Candida albica, S. aureus and E. coli. Jurnal Biologi. p. 11-16

      [2] Arwansyah, Ambarsari , L., Sumaryada, T. I.. 2014. Docking Simulation Curcumin compound and analogs As Androgen Receptor Inhibitor on Prostate Cancer. Current. Biochem. 1 (1): 11-19.

      [3] Asriani, D. 2010. Isolation Xantorizol of Temu Lawak Chosen By Numbers of Hope. Thesis. Faculty of Mathematics and Natural Sciences. Bogor Agricultural

      [4] Davis, W.W., and Stout, T.R., 1971. Disc Plate Methods of Microbiological Antibiotic Essay. J.Applied Microbiology, p. 659-665.

      [5] Fabrega, Anna dkk. 2009. Mechanism of action of and resistance to quinolones. Microbial Biotechnology, 2(1): 40–61.

      [6] Hallman J, Quadt-Hallman A, Mahaffee WF, Kloepper JW. 1997. Bacterial endophytes in agricultural crop. Can J Microbiol. Vol.43, p.895-914.

      [7] Hwang JK, Shim JS, Baek NI, Pyun YR. 2000. Xanthorrhizol: a potential antibacterial agent from C. xanthorrhiza against S. mutans. Planta Med 2000.Vol. 66,.2(196-197).

      [8] Herdiyanto. 2014. Optimisation Methods of Extraction and Isolation Xantorizol From Temulawak (Curcuma xanthorrhiza Roxb.). Paper. Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University.

      [9] Hong, N.M and Sirat. 2004. Synthesis of several bisabolane sesquiterpenoids from xanthorrhizol isolated from Curcuma xanthorrhiza and their bioactivities. The 4th Annual Seminar of National Science Fellowship

      [10] Inamandar, A.C and Paliit,A. 2003. The genus Malassezia ang human disease. Indian J.Dermatol Venereol Leprol

      [11] Kusumaningtyas, E., Astuti, E., and Darmono. 2008. Sensitivity Method Contact Bioautografi and agar Overlay in Determining Compound Antifungi. Indonesian Journal of Pharmaceutical Sciences. Vol. 6, No. 2, p. 75-79

      [12] Lipinski CA. 2004. Lead- and drug-like compounds: the rule-of-five revolution. J..Drug Discovery Today: Technologies. vol.1 no 4g.Discovery004iscovery

      [13] Mohamed, M. A.,Ghanem,H.W, El-Ghaffar, A. and Mohamed, S.S. 2013. Biological Evaluation and Molecular Docking of Substituted Quinazolinones as Antimicrobial Agents. Australian Journal of Basic and Applied Sciences, 7(2): 263-274.

      [14] Oon, S.F., Nallappan , M., Tee, T.T, et al . 2015. Xanthorrhizol: a review of its pharmacological activities and anticancer properties. Cancer Cell Int (2015) 15:100

      [15] Sherma, J. and B. Fried. 2003. Handbook of Thin Layer Cromatography. New York: Marcel Dekker, Inc.

      [16] Strobel, G.A. 2002. Microbial Gifts From Rainforests. Can. J. Plant Phathol. Vol. 24. No. 14-20

      [17] Shibuya H, Agusta A, Ohashi K, Maehara S, and Simanjuntak P. 2005. Biooxidation of (+)-catechin and (-)-epicatechin into 3,4-dihydroxy flavan derivatives by the endophytic fungus Diaporthe sp. Isolated from a tea plant. Chem Pharm Bull. Vol.53, No.7, Hal.866-867

      [18] Siagian M.H. 2006. Temulawak as a plant Drugs and cultivation intensively. Bogor. Balitbang Botany, LIPI Research and Development Center.

      [19] Utami, U. 2011. Isolation, identification and antimicrobial activities selection of endophytic bacterial from mangrove Bruguiera gymnorrhiza. International Journal of Academic Research.vol.3,p.187-194

      [20] Veber DF, Johnson SR, Cheng HY, Smith BR. 2002. Molecular properties that influence the oral bioavailability of drug Candidates. J. Med.Chem. 45: 2615-2623.

      [21] Yang, Xiang, Xue Zhang, Shui-Ping Yang and Xiao-Dong Yu. 2013. Preliminary Antibacterial Evaluation of the Chemical Compositions in Herba pogostemonis Oil. J. Pharm. Sci, 26 (6): 1173-1179

      [22] Yulianty, R., Rante, H.,Alam,G. and tahir,A. 2011. Screening and analysis of TLC bioautography of antimicrobial compounds of several sponge extracts from the waters of Barrang Lompo Island, South Sulawesi. Traditional medicine magazine. Vol.16, no.2, p 88-94.

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  • How to Cite

    Utami, U., Fuadati, C., & Ida Alfiah, C. (2019). Antibacterial Activities Test of the Xanthorrhizol Bioactive Compound in the Endophytic Bacteria of Curcuma xanthorrhiza. International Journal of Engineering & Technology, 8(1.9), 283-286. https://doi.org/10.14419/ijet.v8i1.9.26670

    Received date: 2019-01-29

    Accepted date: 2019-01-29