Role of Zn-Regulated Transporterand Iron (Fe)-Regulated Transporter-Like Protein (ZIP) Gene Family in Rice(Oryzasativa.L) in Foliar Application of Zinc as Bio Fortification Strategy to Enhance Grain Zinc Content

  • Authors

    • Shobha Deepak H. S
    • Gopinath S.M
    • Shankar A.G
    • Rameshraddy. .
    2018-11-30
    https://doi.org/10.14419/ijet.v7i4.25.26934
  • Biofortification, Foliar Zn application, Zn deficiency, Zn-regulated transporters and iron(Fe)-regulated transporter-like protein(ZIP), Oryzasativa.L

  • Abstract

    Cereal crops especially rice (Oryza sativa L.) play an important role in satisfying daily calorie intake in developing world, but they are inherently very low in zinc concentrations, more so when grown on zinc deficient soils. Biofortification is one among various immediate strategies for overcoming Zn deficiency in humans. Availability of soil applied zinc to the plant is limited by chemical  and physical soil properties and hence foliar application of Zn is an effective strategy. Mobilization of foliar applied zinc and remobilization of plant accumulated zinc to grain is dependent on Zinc(Zn)-regulated transporter and iron(Fe)-regulated transporter-like protein(ZIP) gene family. Here we discuss the expression pattern of OsZIP1, OsZIP2, OsZIP3, OsZIP4, OsZIP5 and OsZIP7  transportersin two different seed zinc genotypes(high grain zinc content types and low grain  zinc content  types) at vegetative stage(65DAS) and at reproductive stage(50% panicle initiation)in stem, leaves and reproductive parts in response to Zn foliar applicationin the form of ZnSO4.7H2Oas an essential advance for understanding and manipulating the Zn absorption and translocation in rice. RT-PCR analysis  at 65DAS showed OsZIP1, OsZIP2, OsZIP7  expression  to be very minimal in leaves. OsZIP3  andOsZIP4 expression was medium to highindicatingOsZIP3 and OsZIP4 to be actively involved in mobilization of foliar applied zinc from leaves.OsZIP1, OsZIP3 and OsZIP4 had higher activity at reproductive stage in leaf compared to vegetative stage suggesting their involvement in zinc remobilization/transport during reproductive stage.OsZIP4 and OsZIP7 hadhigher expression activity in  developing grains.There was no difference observed in expression levels of transporters among zinc types. There was large variability observed in zinc mobilization due to transporter function specificity and it is possible  to enhance Zn content in grains under zinc sufficient conditions by direct foliar application at grain filling stage as transporters would facilitate this mobilization.

     

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  • How to Cite

    Deepak H. S, S., S.M, G., A.G, S., & ., R. (2018). Role of Zn-Regulated Transporterand Iron (Fe)-Regulated Transporter-Like Protein (ZIP) Gene Family in Rice(Oryzasativa.L) in Foliar Application of Zinc as Bio Fortification Strategy to Enhance Grain Zinc Content. International Journal of Engineering & Technology, 7(4.25), 256-261. https://doi.org/10.14419/ijet.v7i4.25.26934

    Received date: 2019-01-31

    Accepted date: 2019-01-31

    Published date: 2018-11-30