Role of Zn-Regulated Transporterand Iron (Fe)-Regulated Transporter-Like Protein (ZIP) Gene Family in Rice(Oryzasativa.L) in Foliar Application of Zinc as Bio Fortification Strategy to Enhance Grain Zinc Content

  • Authors

    • Shobha Deepak H. S
    • Gopinath S.M
    • Shankar A.G
    • Rameshraddy. .
    2018-11-30
    https://doi.org/10.14419/ijet.v7i4.25.26934
  • Biofortification, Foliar Zn application, Zn deficiency, Zn-regulated transporters and iron(Fe)-regulated transporter-like protein(ZIP), Oryzasativa.L

  • Cereal crops especially rice (Oryza sativa L.) play an important role in satisfying daily calorie intake in developing world, but they are inherently very low in zinc concentrations, more so when grown on zinc deficient soils. Biofortification is one among various immediate strategies for overcoming Zn deficiency in humans. Availability of soil applied zinc to the plant is limited by chemical  and physical soil properties and hence foliar application of Zn is an effective strategy. Mobilization of foliar applied zinc and remobilization of plant accumulated zinc to grain is dependent on Zinc(Zn)-regulated transporter and iron(Fe)-regulated transporter-like protein(ZIP) gene family. Here we discuss the expression pattern of OsZIP1, OsZIP2, OsZIP3, OsZIP4, OsZIP5 and OsZIP7  transportersin two different seed zinc genotypes(high grain zinc content types and low grain  zinc content  types) at vegetative stage(65DAS) and at reproductive stage(50% panicle initiation)in stem, leaves and reproductive parts in response to Zn foliar applicationin the form of ZnSO4.7H2Oas an essential advance for understanding and manipulating the Zn absorption and translocation in rice. RT-PCR analysis  at 65DAS showed OsZIP1, OsZIP2, OsZIP7  expression  to be very minimal in leaves. OsZIP3  andOsZIP4 expression was medium to highindicatingOsZIP3 and OsZIP4 to be actively involved in mobilization of foliar applied zinc from leaves.OsZIP1, OsZIP3 and OsZIP4 had higher activity at reproductive stage in leaf compared to vegetative stage suggesting their involvement in zinc remobilization/transport during reproductive stage.OsZIP4 and OsZIP7 hadhigher expression activity in  developing grains.There was no difference observed in expression levels of transporters among zinc types. There was large variability observed in zinc mobilization due to transporter function specificity and it is possible  to enhance Zn content in grains under zinc sufficient conditions by direct foliar application at grain filling stage as transporters would facilitate this mobilization.

     

  • References

    1. [1] Welch RM and Graham RD. A new paradigm for world agriculture: meeting human needs -productive, sustainable, nutritious. Field crops Research. 1999 Jan 01;60: 1-10.

      [2] Graham RD, Welch RM, Bouis HE. Addressing micronutrient malnutrition through enhancing the nutritional quality of staple foods: principles, perspectives and knowledge gaps. Adv. Agron. 2001;70: 77–142.

      [3] WELCH RM, GRAHAM RD. Breeding for micronutrients in staple food crops from a human nutrition perspective. Journal of Experimental Botany. 2004 Feb 01;55: 353-364.

      [4] Wissuwa M, Ismail AM and Yanagihara S. Effects of Zinc Deficiency on Rice Growth and Genetic Factors Contributing to Tolerance. Plant Physiology, 2006 Oct;142(2): 731-741.

      [5] Yilmaz AH, Ekiz B, Torun I, Gultekin S, Karanlik SA, Bagci, Cakmak I. Effect of different zinc application methods on grain yield and zinc concentration in wheat cultivars grown on zincâ€deficient calcareous soils. Journal of Plant Nutrition. 1997; 20(4-5): 461-471

      [6] S. Rohini, M. Sharanya, A. Vidhya, S. Viji and P. Poornima. "Proximity Coupled Microstrip Antenna for Bluetooth, WIMAX and WLAN Applications ." International Journal of Communication and Computer Technologies 5 (2017), 48-52.

      [7] Guerinot ML. The ZIP family of metal transporters. BiochimicaBiophysicaActa. 2000 May 01;1465(1-2):190-198

      [8] Ramesh SA, Shin R, Eide DJ and Schachtman DP. Differential metal selectivity and gene expression of two zinc transporters from rice. Plant Physiology. Sept 2003;133(1): 126-134.

      [9] Nagarathna TK, Shankar AG, Udayakumar M. Assessment of genetic variation in zinc acquisition and transport to seed in diversified germplasm lines of rice (Oryza sativa L.). Journal of Agricultural Technology. 2010; 6(1): 171-178.

      [10] Piper CS. Soil and Plant Analysis. Hans Publishers, Bombay. 1966.

      [11] Datta K, Schmidt A, Marcus A. Characterization of two soybean repetitive proline-rich proteins and a cognate cDNA from germinated axes. Plant Cell. 1989 Sept;1(9):945-952

      [12] Hegazy, A. A. E. Coarse Grains Distribution Of The Natural Deposits In Arid Areas.

      [13] Bailey TL, Williams N, Misleh C, Li WW. MEME: discovering and analyzing DNA and Protein sequence motifs. Nucleic Acids Res. 2006 Jul 01;34(2):W369-W373

      [14] Grotz N, Fox T, Connolly E, Park W, Guerinot M L and Eide D. Identification of a family of zinc transporter genes from Arabidopsis that respond to zinc deficiency. Proceedings of the National Academy of Sciences.1998 Jun 09;95(12): 7220-7224

      [15] Vert G, Grotz N, Dedaldechamp F, Gaymard F, Guerinot ML, Briat IF and Curie C. IRTl, an Arabidopsis transporter essential for iron uptake from the soil and for plant growth. The Plant Cell. 2002 Jun 06;14: 1223-1233.

      [16] Moreau S, Thomson RM, Kaiser BN, Trevaskis B, Guerinot ML, Udvardi MK, Puppo A, Day DA. GmZIPl encodes a symbiosis-specific zinc transporter in soybean. Journal of Biological Chemistry. 2002 Feb 15;277: 4738-4746.

      [17] Schachtman DP,SusanA, BarkerBJ. Molecular approaches for increasing the micronutrient density in edible portions of food crops. Field Crops Research.1999 Jan;60(1-2):81-92

      [18] Eide DJ. Zinc transporters and the cellular trafficking of zinc.Biochimica et BiophysicaActa. 2006 Jul;1763(7): 711–722

      [19] Ishimaru Y, Suzuki M, Kobayashi T, Akahashi M, Nakanishi H, Mori S, Nishizawa NK. OsZIP4, a novel zinc-regulated zinc transporter in rice. Journal of Experimental Botany, 2005 Nov 01;56(422): 3207-3214.

      [20] Mabesaa RL, Impaa SL, Grewalb D, Johnson-Beebouta SE.Contrasting grain-Zn response of biofortification rice (Oryza sativa L.)breeding lines to foliar Zn application.Field Crops Research 2013 Aug 01;149: 223–233.

  • Downloads

  • How to Cite

    Deepak H. S, S., S.M, G., A.G, S., & ., R. (2018). Role of Zn-Regulated Transporterand Iron (Fe)-Regulated Transporter-Like Protein (ZIP) Gene Family in Rice(Oryzasativa.L) in Foliar Application of Zinc as Bio Fortification Strategy to Enhance Grain Zinc Content. International Journal of Engineering & Technology, 7(4.25), 256-261. https://doi.org/10.14419/ijet.v7i4.25.26934