Evaluation of Potential Native Phosphate Solubilizing Bacteria for the Agriculture Practice of okra (Abelmoschus Esculentus) with the Target to Replace Chemical Fertilizer - in a Field Study

  • Authors

    • P Sajeesh
    • P Paulraj
    • V Jaamuna
    • N Jayakumar
    • K P Sajna
    • M Chandramohan
    • T Ketharin
    • P T Mohamed Javad
    2019-12-24
    https://doi.org/10.14419/ijet.v7i4.14.27468
  • Phosphate Solubilizing Bacteria (PSB), Triple Super Phosphate (TSP), Rock Phosphate (RP), Tea dust, Eggshells and Bone waste of animal (TEB).
  • Abstract

    Phosphorus (P) is a major growth limiting nutrient. Phosphate Solubilizing Bacteria (PSB) plays an important role in the development of agricultural industry. In this study, the native PSB were isolated from the okra plant root by using Pikovskaya’s medium and potential strains were selected based on the phosphate solubilization efficiency. The potential strains in presence of Rock Phosphate (RP), Tea dust, Eggshells and Bone waste of animal (TEB) were studied as an alternative fertilizer instead of chemical fertilizer. The PSB consortia strains were prepared and inoculated to the plant root. Different growth parameters were studied to determine the effectiveness of strains. In all cases the difference was significant with p<0.05. A decreasing trend of results were recorded among the tested parameters from plants in PSB with RP, PSB with TEB, and PSB with TSP and followed by PSB without fertilizer. The highest results were recorded among plants in PSB with RP for mean shoot length (64cm), mean weight of fruits (29gm), mean length of fruits (23cm), mean number of leaves (13Nos) and chlorophyll content (5.1mg/g). Hence, the present study showed that the application of PSB with the cheap source of nutrients has shown the capability of providing similar or enhanced results based on the various characters expressed by the plant and thus reduce the reliance on synthetic chemical fertilizers.

     

  • References

    1. [1] Mal B, Mahapatra. P and Mohanty S (2014), "Effect of Diazotrophs and Chemical Fertilizers on Production and Economics of Okra (Abelmoschus esculentus, L.) Cultivars," American Journal of Plant Sciences, Vol. 5 No. 1, pp. 168-174.

      [2] Binalfew T and Alemu Y (2016), Characterization of Okra (Abelmoschus esculentus (L.) Moench) Germplasms Collected from Western Ethiopia, International Journal of Research in Agriculture and Forestry, 3(2).

      [3] Jonathan Eshiet, A. and Andi Brisibe, E. (2015), Morphological Characterization and Yield Traits Analysis in Some Selected Varieties of Okra (Abelmoschus Esculentus L. Moench), Advances in Crop Science and Technology, 04(01).

      [4] Semwal DK, Semwal RB, Combrinck S, and Viljoen A, Myricetin: A Dietary Molecule with Diverse Biological Activities. Nutrients, [e-journal] 8(2), 90, (2016), available online: https://dx.doi.org/10.3390/nu8020090, last visit: 19.08.18.

      [5] Karpagam T and Nagalakshmi P (2014), Isolation and characterization of Phosphate Solubilizing Microbes from Agricultural soil, International Journal of Current Microbiology and Applied Sciences, 3(3), pp.601-614.

      [6] Wang T. Camps-Arbestain M, Hedley M and Bishop P (2012), Predicting phosphorus bioavailability from high-ash biochars. Plant and Soil, 357(1-2), 173-187.

      [7] Anand K, Kumari B and Mallick M (2016), Phosphate Solubilizing Microbes: An Effective and Alternative Approach as Biofertilizers. International Journal of Pharmacy and Pharmaceutical Science, 8(2), pp.37-40.

      [8] Huey T & Ibrahim A, Statistical analysis of annual rainfall patterns in peninsular Malaysia using TRIMM algorithm, The 33rd Asian conference and remote sensing, Thailand,(2012), https://www.researchgate.net/publication/260593838, last visit: 16.08.18.

      [9] Mekki BE (2016). Effect of bio-organic, chemical fer-tilizers and their combination on growth, yield and some macro and micronutrients contents of faba bean (Vicia faba L.), Bioscience Research, 13, pp.08-14.

      [10] Ch’ng H, Ahmed O, and Majid N (2014), Improving Phosphorus Availability in an Acid Soil Using Organic Amendments Produced from Agroindustrial Wastes, The Scientific World Journal, 2014, pp.1-6.

      [11] Kivela J (2015), Effects of meat bone meal as fertilizer on leaf nitrogen status in sugar beet and on soil nitrate concentrations, Acta fytotechnica et zootechnica, 18(Special Issue), pp.71-73.

      [12] Farahan R, Supri A, and Teh P (2015), Tensile and Water Absorption Properties of Eggshell Powder Filled Recycled High-Density Polyethylene/Ethylene Vinyl Acetate Composites: Effect of 3-Aminopropyltriethoxysilane, Journal of Advanced Research in Materials Science, 5(1), pp.1-9.

      [13] Ahamed M and Kibret M (2014), Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. Journal of King Saud University - Science, 26(1), pp.1-20.

      [14] Goldstein AH (1986) Bacterial solubilization of mineral phosphates: Historical perspective and future prospects. American Journal of Alternative Agriculture 1:51-7.

      [15] Richardson A and Simpson R (2011), Soil Microorganisms Mediating Phosphorus Availability, American Society of Plant Biologists, Plant Physiology, 156, 989–996.

      [16] Pikovskaya, R.I (1948) Mobilization of P in soil in connection with the vital activity of some microbial species, Microbiologiya 17: 362-370

      [17] Trivedy PK, Goel PK, Chemical and biological methods for water pollution studies, Series in Methodology, Environmental Publications, Karad, (1986), pp.126-127.

      [18] Liba C M Ferrara F I S Manfio G P Fantinatti-Garboggini F Alburquerque R C Pavan C Ramos P L Moreira-Filho C A and Barbosa H R 2006 Journal of Applied Microbiology 101, 1076-1086.

      [19] Sharma K, Dak G, Agrawal A, Bhatnagar M, and Sharma R (2007), Effect of phosphate solubilizing bacteria on the germination of Cicer arietinum seeds and seedling growth, Journal of Herbal Medicine and Toxicology, 1, 61-63.

      [20] Zaidi A, and Khan M S (2006), Co-inoculation effects of phosphate solubilizing microorganisms and Glomus fasciculatumon on green gram - Bradyrhizobium symbiosis, Turkish Journal of Agriculture and Forestry, 30,223-230.

      [21] Gusain Y S, Kamal R, Mehta C M, Singh U S, Sharma A K (2015), Phosphate solubilizing and indole-3-acetic acid producing bacteria from the soil of Garhwal Himalaya aimed to improve the growth of rice, Journal of Environmental Biology, 36, 301–307.

      [22] Hussain M I, Asghar H N, Akhtar M J, and Arshad (2013), Impact of phosphate solubilizing bacteria on growth and yield of maize. Plant Soil and Environment, 32(1), 71-78.

      [23] Singh A V, Chandra R, and Reeta G(2013), Phosphate solubilization by Chryseobacterium sp. and their combined effect with N and P fertilizers on plant growth promotion, Archives of Agronomy and Soil Science, 59(5), 641– 651.

      [24] Ajay Veer Singh, Birendra Prasad and Reeta Goel (2018), Plant Growth Promoting Efficiency of Phosphate Solubilizing Chryseobacterium sp. PSR 10 with Different Doses of N and P Fertilizers on Lentil (Lens culinaris var. PL-5) Growth and Yield, International journal of current microbiology and applied science, 7(5), 2280-2289

      [25] Singh A V and Prasad B (2014), Enhancement of plant growth, nodulation and seed yield through Plant Growth Promoting Rhizobacteria in Lentil (Lens culinaris Medik cv. VL125), International journal of current microbiology and applied science,3(6), 614-622

      [26] Wang M, Zheng Q, Shen Q and Guo S (2013), the critical role of potassium in plant stress response, International journal of molecular science, 14, 7370–7390.

      [27] Sreedevi Sarsan. 2016. Effect of Phosphate Solubilising Bacteria Bacillus PSB24 on Growth of Tomato Plants. Int.J.Curr.Microbiol.App.Sci. 5(7): 310-320.

      [28] Meena R, Kumar S, Maji S, Kumar D and Kumar M (2014), Effect of organic manures and biofertilizers on growth, flowering, yield and quality of tomato cv. Pusa Sheetal, International Journal of Agricultural Sciences, 10(1), pp.329-332.

  • Downloads

  • How to Cite

    Sajeesh, P., Paulraj, P., Jaamuna, V., Jayakumar, N., P Sajna, K., Chandramohan, M., Ketharin, T., & T Mohamed Javad, P. (2019). Evaluation of Potential Native Phosphate Solubilizing Bacteria for the Agriculture Practice of okra (Abelmoschus Esculentus) with the Target to Replace Chemical Fertilizer - in a Field Study. International Journal of Engineering & Technology, 7(4.14), 40-45. https://doi.org/10.14419/ijet.v7i4.14.27468

    Received date: 2019-02-15

    Accepted date: 2019-02-15

    Published date: 2019-12-24