An in Vitro Analysis of Antibacterial Efficacy of Local Brands of Toothpastes and Mouthrinses in Malaysian Market on Selected Oral Pathogens

  • Authors

    • M Chandramohan
    • S W Chan
    • P Paulraj
    • P T Mohamed Javad
    • P Sajeesh
    • K P Sajna
    • T Ketharin
    2019-12-24
    https://doi.org/10.14419/ijet.v7i4.14.27471
  • Antimicrobial efficacy, Cetylpyridium chloride, Fluoride, Mouthrinse, Toothpaste.

  • Abstract

    Oral disease affects a considerable portion of population and is considered one of the major causes of tooth loss in developed and developing countries. An in vitro study was conducted to investigate the antimicrobial efficacy of toothpastes and mouthrinses towards oral pathogens which are found to cause most of the oral diseases such as gingivitis and dental plague. In this study, a total of five toothpastes and four mouthrinses were investigated for their antimicrobial activity against five oral pathogens such as Streptococcus salivarius, Streptococcus sanguis, Streprococcus oralis, Streptococcus mutans and Candida albicans. The efficacy of different concentration of the toothpastes and mouthrinses were assessed by agar well diffusion method. Statistical analysis was performed by using analysis of variance (ANOVA) with post-hoc least square differences (LSD) method (p=0.05). Toothpaste B gave the maximum zone of inhibition against tested organisms, Streptococcus sanguis and Streptococcus oralis. Toothpaste C and E gave the maximum zone of inhibition against Streptococcus salivarius and Streptococcus mutans respectively. Toothpaste A was most effective against Candida albicans. Mouthrinse G was most effective against Streptococcus salivarius, Streptococcus sanguis, Streptococcus oralis and Candida albicans. In conclusion, the present study has demonstrated that dentifrices which contain fluoride and cetylpyridium chloride formulation gave the maximum zone of inhibition against the tested organisms compared to other active ingredients.

  • References

    1. [1] Preston AJ (1998), A review of dentifrices. Dental update 25, 247-253.

      [2] World Health Organization. 2004. Research for oral health in developing countries-challenges for the 21st Century and the approach of the WHO Global Oral Health Programme. Global Forum for Health Research 8, Mexico City.

      [3] Taylor GW, Loesche WJ, Terpenning MS (2000), Impact of oral diseases on systemic health in the elderly: diabetes mellitus and aspiration pneumonia. Journal of Public Health and Dentistry 60, 313-320.

      [4] Hojo S, Takahashi N, Yamada T (1991), Acid profile in carious dentin. Journal of Dental Research 70, 1 82-186.

      [5] Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE (2005), Defining the normal bacterial flora of the oral cavity. Journal of Clinical Microbiology 4, 5721-5732.

      [6] Bagg J (1990), Can the colonization resistance of the oral microflora be reduced? Microbial Ecology in Health and Disease 3, v-viii.

      [7] Almas K, Dahlan A, Mahmoud A (2001), Propolis as a natural remedy; An update. Saudi Dental Society 13, 45-49.

      [8] Health Promotion Board (HPB). Dental Carries affiliated with the Singapore government. 2006. Accessed on 25/08/2017

      [9] Kanchanakamol U, Umpriwan R, Jotikasthira N, Srisilapanan P, Tuongratanaphan S, Sholitkul W. (1995), Reduction of plague formation and gingivitis by a dentifrices containing triclosan and copolymer. Journal of Periodontology 66,109-112.

      [10] Redmond CA, Blinkhorn FA, Kay EJ, Davies RM, Worthington HV, Blinkhorn AS (1999), A cluster randomized controlled trial testing the effectiveness of school-based dental health education program for adolescent. Journal of Public Health and Dentistry 59, 12-17.

      [11] Varenne B, Petersen PE, Ouattara S. (2006) Oral health behavior of children and adults in urban and rural areas of Burkina Faso, African Dentistry Journal 56, 61-70.

      [12] Mandel ID (1979), Dental Caries. American Science 67, 680-688.

      [13] Saini S, Aparna GN, Mahajan A, Arora DR (2003), Microbial infection in orodental infection. Indian Journal of Medical Microbiology. 21, 111-114.

      [14] Maeda K, Nagata H, Kuboniwa M. (2013), Identification and Characterization of Porphyromonas gingivalis Client Proteins That Bind to Streptococcus oralis Glyceraldehyde-3-Phosphate Dehydrogenase. Infection and Immunity. 81, 753-763.

      [15] Xu P, Alves JM, Kitte T, Brown A, Chen Z, Ozaki LS, Manque P, Ge X, Serrano MS, Puiu D, Hendricks S, Wang Y, Chaplin MD, Akan D, Paik S, Peterson DL, Macrina FL, Buck GA (2007), Genome of the Opportunistic Pathogen Streptococcus sanguinis. Joutrnal of Bacteriology 189, 3166-3175.

      [16] Prashanth M (2011), Antimicrobial efficacy of different toothpastes and mouthrinse: an in vitro study. Dental research Journal. 8, 85-94.

      [17] Roopavathi KM, Venu GS, Pushpalatha G, Bennadi D, Renushri BV, Madhura AS (2015), Antimicrobial efficacy of commercially available toothpastes-an in vitro study. Journal of Young Pharmacist. 7, 188-189.

      [18] Eric CR (1994), Assessment of antimicrobial efficacy of fluoride pastes in school children’s. Australian Prescriber. 17,49-51.

      [19] Addy M, Moran J (2008), Chemical supragingival plaque control. In. Lindhe J, Lang NP, Karring T. Clinical periodontology and implant dentistry. Oxford: Blackwell Munkgaard. 734-783

      [20] Regos J, Hitz HR (1974) Investigation on the mode of action of triclosan, a broad-spectrum antimicrobial agent. Zentbl. Bakteriol Parasitenkd. Infektkrankh. Hyg. Abt. 1 Orig. Reihe A. 226, 390-401.

      [21] Dr Bora A, Dr Goswami A, Dr Kundu GK, Dr Ghosh B (2014), Antimicrobial Efficacy of few commercially available herbal and non-herbal toothpastes against clinically isolated human cariogenic pathogens. Journal of Nepal Dentistry Association. 14, 36.

      [22] Sohail SK (2003), Assessment of Antibacterial potential of different Toothpastes and Toothpowders against Mouth Flora. Indian Journal of Research. 2, 180-181

      [23] Matthews RW (2003) Hot salt water mouth baths. British Dentistry Journal. 19, 51-53.

      [24] Gibraiel F, Rajput M, Rajput MS, Manisha S, Saxena N, Vishal A, Jha AK (2014), In Vitro Study to Investigate the Antimicrobial Efficacy of Different Toothpastes and Mouth Rinses. Research Journal of Pharmaceutical, Biological and Chemical Science. 5, 246-257.

      [25] Addy M., Dummer PMH, Hunter ML, Kingdon A, Shaw MC (1990), The effect of toothbrushing frequency, toothbrushing hand, sex and social class on the incidence of plaque, gingivitis and pocketing in adolescents: A longitudinal cohort study. Community Dental Health 7, 237-247.

      [26] Feather JD (2004), The continuum of dental caries-evidence for a dynamic disease process. Journal of Dental Research. 83, C39-C42.

      [27] Kay E, Locker D (1998), A systematic review of the effectiveness of health promotion aimed at improving oral health. Community Dental Health 15,132-144.

      [28] Sheiham A (2001), Dietary effects on dental diseases. Public Health and Nutrition 4, 569-591.

      [29] Eaton KA, Carlile MJ (2008), Tooth brushing behaviour in Europe: opportunities for dental public health. International Dentistry Journal. 58, 287-293.

      [30] Santos AP, Nadanovsky P, Oliveira BH (2013) A systematic review and meta-analysis of the effects of fluoride toothpastes on the prevention of dental caries in the primary dentition of preschool children. Community Dentistry and Oral Epidemiology 41, 1-12

      [31] Cenci MS, Tenuta LMA, Pereira-Cenci T, Del Bel Cury AA, ten Cate JM, Cury JA (2008), Effect of microleakage and fluoride on enamel-dentine demineralization around restorations. Caries Research 42, 369-379.

      [32] Cury JA, Amaral RC, Tenuta LMA, Del Bel Cury AA, Tabchoury CPM (2010), Low-fluoride toothpaste and deciduous enamel demineralization under biofilm accumulation and sucrose exposure. European Journal of Oral Sciences. 118, 370-375.

      [33] Davies RM, Ellwood RP, Davies GM (2004), The effectiveness of a toothpaste containing triclosan and prolyvinyl-methyl ether maleic acid copolymer in improving plaque control and gingival health: a systematic review. Journal of Clinical Periodontology. 31, 1029-1033.

      [34] Flisfisch S, Meyer J, Meurman JH, Waltimo T (2008), Effects of fluorides on Candida albicans. Oral Diseases 14, 296-301.

      [35] Adwan G, Salameh Y, Adwan K, Barakat A (2012), Assessment of antifungal activity of herbal and conventional toothpastes against clinical isolates of Candida albicans. Asian Pacific Journal of Tropical Biomedicine 2, 375-379.

      [36] Amrutesh S, Malini J, Tandur PS, Patki PS (2010), Clinical evaluation of a novel herbal dental cream in plaque formation: a double-blind, randomized, controlled clinical trial. Journal of Experimental Pharmacology 2, 105-109.

      [37] Hassan SH (2012), A review on miswak (Salvadora persica) and its effect on various aspects of oral health. Saudi Dental Journal 24, 63-69.

      [38] Olsson B (1978), Efficiency of traditional chewing sticks in oral hygiene programs among Ethiopian schoolchildren. Community Dentistry and Oral Epidemiology 6, 105–109.

      [39] Char D, Dogao A, Dogan M (1987), Evaluation of Middle Eastern toothbrush “Salvadora persicaâ€. Journal of Electro Micro Techniques. 5, 145.

      [40] Danielsen B, Baelum V, Manji F, Fejerskov O (1989), Chewing sticks, toothpaste, and plaque removal. Acta. Odontologica Scandinavica. 47, 121–125.

      [41] Almas K, Al-Zeid Z (2004), The immediate antimicrobial effect of a toothbrush and miswak on cariogenic bacteria: a clinical study. Jounal of Contemporary Dental Practices. 5, 105–114.

      [42] Al-Bagieh NH, Idowu A, Salako NO (1994), Effect of aqueous extract of miswak on the in vitro growth of Candida albicans. Microbios. 80, 107–113.

      [43] Almas K, Al-Bagieh NH (1999), The antimicrobial effects of bark and pulp extracts of miswak, Salvadora persica. Biomedical Letters. 60, 71– 75.

      [44] Asadoorian J, Williams K (2008), Cetylpyridinium chloride mouth rinse on gingivitis and plaque. Journal of Dental Hygiene. 82, 82-84

      [45] Mankodi S, Bauroth K, Witt JJ, Bsoul S, He T, Gibb R, Dunavent J, Hamilton A (2005), A 6-month clinical trial to study the effects of a cetylpyridinium chloride mouth rinse on gingivitis and plaque. American Journal of Dentistry 18, 9A-14A.

      [46] Giuliana G, Pizzo G, Milici ME, Musotto GC, Giangreco R (1997), In vitro antifungal properties of mouthrinses containing antimicrobial agents. Journal of Periodontology 68, 729-733.

      [47] Giuliana G, Pizzo G, Milici ME, Giangreco R (1999), In vitro activities of antimicrobial agent’s againsts. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 87, 44-49.

      [48] Hu D, Li X, Sreenivasan PK, DeVizio W (2009), A randomized, double-blind clinical study to assess the antimicrobial effects of a cetylpyridinium chloride mouth rinse on dental plaque bacteria. Clinical Therapy 31, 2540-2548.

      [49] Costa X, Laguna E, Herrera D, Serrano J, Alonso B, Sanz M (2013) Efficacy of a new mouth rinse formulation based on 0.07% cetylpyridinium chloride in the control of plaque and gingivitis: a 6-month randomized clinical trial. Journal of Clinical Periodontology 40, 1007-1015.

      [50] Edlind MP, Smith WL, Edlind TD (2005), Effects of cetylpyridinium chloride resistance and treatment on fluconazole activity versus Candida albicans. Antimicrobial Agents and Chemotherapy 49, 843-845.

      [51] Samaranayake LP, Holmstrup P (1989), Oral candidiasis and human immunodeficiency virus infection. Journal of Oral Pathology and Medicine. 18, 554-564.

      [52] Majed MM, Shadi FG, Karem HA, Sayer IA, Wasfi MO (2013) Antimicrobial activity of common mouthwash solutions on multidrug-resistance bacterial biofilms. Journal of Clinical Medicine and Research. 5, 389-394.

      [53] Neeraja R, Anantharaj A, Praveen P, Karthik V, Vinitha M. (2008), The effect of povidone-iodine and chlorhexidine mouth rinses on plaque Streptococcus mutans count in 6- to 12-year-old school children: an in vivo study. Journal Indian Society of Pedodonitics and Preventive Dentistry 26, S14-S18.

      [54] Trombetta D, Castelli F, Sarpietro MG, Venuti V, Cristani M, Daniele C, Saija A, Mazzanti G, Bisignano G (2005), Mechanisms of antibacterial action of three monoterpenes. Antimicrobial Agents and Chemotherapy 49, 2474–2478.

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  • How to Cite

    Chandramohan, M., W Chan, S., Paulraj, P., T Mohamed Javad, P., Sajeesh, P., P Sajna, K., & Ketharin, T. (2019). An in Vitro Analysis of Antibacterial Efficacy of Local Brands of Toothpastes and Mouthrinses in Malaysian Market on Selected Oral Pathogens. International Journal of Engineering & Technology, 7(4.14), 57-62. https://doi.org/10.14419/ijet.v7i4.14.27471

    Received date: 2019-02-15

    Accepted date: 2019-02-15

    Published date: 2019-12-24