Effect of Different basal Media on Callus Growth and Morphology of Barringtonia Racemosa L Endosperms Explant

  • Authors

    • N D A Kamaruzzaman
    • A Saleh
    • F Pardi
    • N Ahmat
    • N J Sidik
    2019-12-24
    https://doi.org/10.14419/ijet.v7i4.14.27480
  • Barringtonia racemosa L, callus, Murashige and Skoog (MS) medium, Lloyd and McCown Wood Plant (WPM) medium, Gamborg (B5) medium.

  • Abstract

    Barringtonia racemosa L. has many medicinal properties especially the fruit and leaf parts. The fruits are used to relief pain and inflammation, the leaves were proved to control high blood pressure whereas the roots barks are effective to treat chicken pox. Due to its medicinal importance for human health, it is essential to conserve this plant. A comparative study of different types of media was performed to study its effect on callus growth of endosperm explant from B. racemosa. Types of basal media studied including Murashige and Skoog (MS), Lloyd and McCown Woody Plant (WPM) and Gamborg (B5). The endosperm explant of B. racemosa were cultured on different basal MS, WPM and B5 media supplemented with 1.0 mg/L 2,4-D and 1.5 mg/L kinetin. The observation of callus growth and morphological characteristics of callus were done on weekly basis within 4 weeks. After 4 weeks of incubation period, the maximum fresh weight (0.300 ± 0.027g) and dry weight (0.025 ± 0.003g) were recorded from the explants cultured on MS medium followed by WPM and B5 media supplemented with 1.0 mg/L 2,4-D and 1.5 mg/L kinetin. The textures of callus produced from MS were nodular in shape and creamy colour. In conclusion, all the media studied had successfully induced the callus growth of B. racemosa with the present of 1.0 mg/L 2,4-D and 1.5 mg/L kinetin.

     

  • References

    1. [1] Wangchuk, P. (2004). Bioactive alkaloids from medicinal plants of Bhutan, M. Sc. Thesis, Department of Chemistry, University of Wollongong, Australia.

      [2] Ong, H. C., Nordiana, M. (1999). Malay ethno-medicobotany in Machang, Kelantan, Malaysia. Fitotera-pia, (70), 502-513.

      [3] Deraniyagala, S. A., Ratnasooriya, W. D., and Goonasekara, C. L. (2003). Antinociceptive effect and toxicological study of the aqueous bark extract of Barringtonia racemosa on rats. PubLMed.gov, 86(1), 21-6.

      [4] Osman, N. I., Sidik, N. J and Awal, A. (2016). Effects of variations in culture media and hormonal treatments upon callus induction potential in endosperm explant of Barringtonia racemosa L. Asian Pacific Journal of Tropical Biomedicines, (6)2, 143-147.

      [5] Osman, N. I., Sidik, N. J., & Awal, A. (2015). Pharmacological activities of Barringtonia racemosa L.(Putat), a tropical medic-inal plant species. Journal of Pharmaceutical Sciences and Re-search, 7(4), 185.

      [6] Osman, N. I., Sidik, N. J., Awal, A., Adam, N. A. M., & Rezali, N. I. (2016). In vitro xanthine oxidase and albumin denaturation inhibition assay of Barringtonia racemosa L. and total phenolic content analysis for potential anti-inflammatory use in gouty arthritis. Journal of intercultural ethnopharmacology, 5(4), 343.

      [7] Duangporn, P and Siripong. (2009). Effect of auxin and cytokinin in Phyllanthusol a production by callus culture Phyllanthusol acidus skeels. American-Eurasian Journal of Agricultural and Environmental Science, 5(2), 258-263.

      [8] Sauer, M., Robert, S and Klein-Vehn, J. (2013). Auxin: simply complicated. Journal of Experimental Botany, 64(9), 2565-77.

      [9] Ikeuchi, M., Sugimoto, K and Iwase. (2013). A plant callus: mechanisms of induction and repression. The Plant Cell, 25(9), 3159-73.

      [10] Bourgaud, F., Gravot, A., Milesi, S and Gontier, E. (2011). Production of plant secondary metabolities: A historical perspective. Plant Sci, 161, 839-851.

      [11] George, E. F., Hall, M. A and De-Klerk, G. J. (2008). Plant propagation by tissue culture. (1)3, Springer, Dordrecht, Netherlands, pages: 501.

      [12] Staba, E. J. Plant tissue culture as a source of biochemicals. Florida: CRC Press; 1980.

      [13] Amiri, M., Gerdakaneh, M., Mamghani, R and Nouri, F. (2013). Effect of different concentration of 2,4-D on callus induction and callus root indication in 2 explants from true potato (Solanum tuberosum L.) seeds. Scientific Journal of Agronomy and Plant Breeding, 1(2): 56-63.

      [14] Barnes, D. L and Seefeldt, S. (2009). Attenuation and effectiveness of triclopyr and 2,4-D along alaska highway right-of-ways in a continental and a coastal subarctic environment. Alaska Department of T. Environment, 2, 07-06.

      [15] Michel, Z., Hilaire, K. T., Mongomake, K., Georges, A. N and Justin, K. Y. (2008). Effecr of genotypes explants, growth regulators and sugars on callus induction in cotton (Gossypium hirsutum L.). Australian Journal of Crop Science, 2(1), 1-9.

      [16] Jansen, P. C. M. and Cardon, D. (2005). Plant Resources of Tropical Africa/ Resources Vegetables de L’ Afrique Tropicale. Wageningen, Netherlands.

      [17] Avil´es, F., Rios, D,, Gonz´alez, R and S´anchez-Olate, M. (2009). Effect of culture medium in callogenesis from adult walnut leaves (Juglans regia L.). Chilean Journal of Agricultural Research, 69(3), 460-467.

      [18] Behbahani, M., Shanehsazzadeh, M and Hessami, M.J. (2011). Optimization of callus and cell suspension cultures of Barringtonia racemosa (Lecythidaceae family) for lycopene production. Science Agricultural, 68(1), 69-76.

  • Downloads

  • How to Cite

    D A Kamaruzzaman, N., Saleh, A., Pardi, F., Ahmat, N., & J Sidik, N. (2019). Effect of Different basal Media on Callus Growth and Morphology of Barringtonia Racemosa L Endosperms Explant. International Journal of Engineering & Technology, 7(4.14), 102-106. https://doi.org/10.14419/ijet.v7i4.14.27480

    Received date: 2019-02-15

    Accepted date: 2019-02-15

    Published date: 2019-12-24