Callus Formation on Endosperm from Immature and Mature Fruits of Barringtonia Racemosa

  • Authors

    • D S M Soder
    • D N A A Khalid
    • A Saleh
    • F Pardi
    • N J Sidik
    2019-12-24
    https://doi.org/10.14419/ijet.v7i4.14.27508
  • Barringtonia racemosa, callogenesis, endosperm, immature fruits, mature fruits

  • Abstract

    Barringtonia racemosa is mangroves type of plant which had been extensively utilized in conventional practices for relieving ailments of pain and inflammation. Many studies have been done on ethnobotanical profiles, pharmacological activities and chemical compounds in Barringtonia racemosa. However, there is a limited study on callogenesis of this plant particularly from different maturity stage of fruits. The present study is to identify the callogenesis of Barringtonia racemosa from endosperm explants of immature and mature fruits in MS medium supplemented with different concentrations of hormones 2,4-Dichlorophenoxyacetic acid (2,4-D) (0, 0.5, 1.0, 1.5 and 2.0 mg/L) and Kinetin (KIN) (0, 0.5, 1.0, 1.5 and 2.0 mg/L). The optimum hormone combination was found in callus grown on endosperm of immature fruits in MS medium supplemented with 1.5 mg/L 2,4-D and 1.0 mg/L KIN. It was also found that the callus in this treatment grew profusely with highest fresh weight (0.513 ± 0.022 g), 100% callus induction and friable callus texture. The callus fresh weight on endosperm explants was higher in immature fruits compared to mature fruits for all the hormone combinations. Therefore, callogenesis were found more efficient from endosperm explant of immature fruits in Barringtonia racemosa species.

     

     

     

  • References

    1. [1] Osman NI, Sidik NJ & Awal A (2017), Ethnobotanical profiles and phytochemical constituents of Barringtonia racemosa L. for potential scrutiny of bioactive compounds through plant biotechnology. Journal of Natural Remedies 17, 57-68.

      [2] Ong HC & Nordiana M (1999), Malay ethno-medico botany in Machang, Kelantan, Malaysia. Fitoterapia 70, 502-513.

      [3] Osman NI, Sidik NJ & Awal A (2015), Pharmacological activities of Barringtonia racemosa L.(Putat), a tropical medicinal plant species. Journal of Pharmaceutical Sciences and Research 7, 185.

      [4] Osman NI, Sidik NJ, Awal A, Adam NAM & Rezali NI (2016), In vitro xanthine oxidase and albumin denaturation inhibition assay of Barringtonia racemosa L. and total phenolic content analysis for potential anti-inflammatory use in gouty arthritis. Journal of intercultural ethnopharmacology 5, 343.

      [5] Patil KR, Patil CR, Jadhav RB, Mahajan VK, Patil PR & Gaikwad PS (2011), Anti-arthritic activity of bartogenic acid isolated from fruits of Barringtonia racemosa Roxb.(Lecythidaceae). Evidence-Based Complementary and Alternative Medicine, doi: 10.1093/ecam/nep148.785245

      [6] Thomas TJ, Panikkar B, Subramoniam A, Nair MK & Panikkar KR (2002), Antitumour property and toxicity of Barringtonia racemosa Roxb seed extract in mice. Journal of ethnopharmacology 82, 223-227.

      [7] Bhatia S, Sharma K, Dahiya R & Bera T (2015), Plant tissue culture. In: Modern Applications of Plant Biotechnology in Pharmaceutical Sciences, Academic Press, London, UK, pp. 31-107.

      [8] Ikeuchi M, Sugimoto K, Iwase A (2013), Plant callus: mechanisms of induction and repression. The Plant Cell 25, 3159-73.

      [9] Murashige T & Skoog F (1962), A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum 15, 473-497.

      [10] Osman NI, Sidik NJ & Awal A (2016), Effects of variations in culture media and hormonal treatments upon callus induction potential in endosperm explant of Barringtonia racemosa L. Asian Pacific Journal of Tropical Biomedicine 6, 143-147.

      [11] George EF, Hall MA & De Klerk GJ (2008), Plant Propagation by Tissue Culture, Vol. 1, 3rd edn Springer, Dordrecht, Netherlands, pp. 186-375.

      [12] Li F, Cui X, Feng Z, Du X & Zhu J (2012), The effect of 2, 4-D and kinetin on dedifferentiation of petiole cells in Arabidopsis thaliana. Biologia plantarum 56, 121-125.

      [13] Hosseini SZ, Jelodar NB, Rahimian H & Ranjbar G (2015), Effect of different concentrations of kinetin and 2,4,D on callus induction of Citrus rootstock (Citrus sp.). Journal Biological 7, 1045-1050.

      [14] Al-Ani NK & Saad Z (2010), Effect of 2, 4-D and Kin combinations on callus induction on Nigella sativa explants and production of thymol. International Journal for Sciences and Technology 143, 1-14.

      [15] Silva ALCD, Caruso CS, Moreira RDA & Horta ACG (2003), In vitro induction of callus from cotyledon and hypocotyl explants of Glycine wightii (Wight & Arn.) Verdc. Ciência e Agrotecnologia 27, 1277-1284.

      [16] Pirrello J, Bourdon M, Cheniclet C, Bourge M, Brown SC, Renaudin JP, Frangne N & Chevalier C (2014), How fruit developmental biology makes use of flow cytometry approaches. Cytometry Part A 85, 115-125.

      [17] Abul-Soad AA, Markhand GS & Solangi ND (2013, June), Impact of immature fruit size and genotype on direct caullogenesis in monoembryonic-seed mango. In VIII International Symposium on In Vitro Culture and Horticultural Breeding 1083, 85-94.

      [18] Rampadarath S, Puchooa D & Ranghoo-Sanmukhiya M (2014), Optimized in vitro plant regeneration of the biodiesel plant Jatropha curcas L.: the effects of using seeds at different stages of maturity as starting materials. International Journal of Plant Biology 5, 34.

  • Downloads

  • How to Cite

    S M Soder, D., N A A Khalid, D., Saleh, A., Pardi, F., & J Sidik, N. (2019). Callus Formation on Endosperm from Immature and Mature Fruits of Barringtonia Racemosa. International Journal of Engineering & Technology, 7(4.14), 107-111. https://doi.org/10.14419/ijet.v7i4.14.27508

    Received date: 2019-02-18

    Accepted date: 2019-02-18

    Published date: 2019-12-24