Detection of Porcine Adulteration in Cosmetic Cream Formulation via TaqMan Probe Real-Time Polymerase Chain Reaction.

  • Authors

    • S S Abd-Gani
    • S Mustafa
    • M N Mohd Desa
    • N F Khairil Mokhtar
    • U K Hanapi
    • Z Zakaria
    • N Yahaya
    • W M A Wan Sulaiman
    2019-12-24
    https://doi.org/10.14419/ijet.v7i4.14.27509
  • Cosmetic cream, MPRE, TaqMan probe, Real-time PCR, 18S rRNA.

  • The identification of  lard adulterated in cosmetic cream using TaqMan probe real-time Polymerase Chain Reaction was developed in this research. The laboratory prepared cream was formulated with oil in water (o/w) emulsion with the adulteration of 1%, 3% and 5% of lard (w/w). The total DNA from lard-adulterated cosmetic cream was successfully extracted. The detection assay targeting different gene; universal 18S rRNA and porcine-specific multiple porcine repetitive element (MPRE) which employed with the TaqMan probe. This study targeting 187 bp of amplicons for 18S rRNA while 99 bp of amplicons for Porcine-specific probes. A comparison of quantification cycle (Cq) with prior to percentage of adulteration were detected. Lard-adulterated cream was amplified for 18S rRNA while no detection was detected for Porcine-specific probes. The Cq value obtained might be vary as proposed by the hypothesis depending on the total recovery of extracted DNA from the lard-adulterated cream.

     

     

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    S Abd-Gani, S., Mustafa, S., N Mohd Desa, M., F Khairil Mokhtar, N., K Hanapi, U., Zakaria, Z., Yahaya, N., & M A Wan Sulaiman, W. (2019). Detection of Porcine Adulteration in Cosmetic Cream Formulation via TaqMan Probe Real-Time Polymerase Chain Reaction. International Journal of Engineering & Technology, 7(4.14), 112-115. https://doi.org/10.14419/ijet.v7i4.14.27509