Comparative Study on Anti-Acetylcholinesterase and Anti-Inflammatory Activities of Date and Apple Vinegars Fortified with Centella Asiatica

 
 
 
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  • Abstract


    Neuroinflammation and deficiency of cholinergic are major factors of neurodegenerative damage correlated to cognitive impairment in Alzheimer’s disease (AD). We investigated the anti-inflammatory and anti-acetylcholinesterase activities of apple and date vinegars added with Centella asiatica in SH-SY5Y neuroblastoma cells. The neuroprotective effect of apple or date vinegar added with various percentage of C. asiatica (0, 0.5, 2, 5) % w/v was determined in vitro. The methanolic extract of apple vinegar added with 2% C. asiatica (AV-2% CA) and date vinegar added with 2% C. asiatica (DV-2% CA)  extracts showed potent neuroprotective effect. Both extracts were subjected to liquid-liquid partitioning yielded aqueous (H2O: AV/DV-2% CA) and ethyl acetate (EA: AV/DV-2% CA) extracts. Anti-inflammatory response against nitric oxide (NO) of all extracts was measured in LPS-induced SH-SY5Y neuroblastoma cells and percentage inhibition of acetylcholinesterase (AChE) was measured using commercially available test kits. It was found that EA: DV-2% CA showed potent ameliorating effect against LPS-induced inflammation (I50: 563.5 ± 0.13μg/mL) and was also responsible in the AChE inhibition activity (IC50: 9.087 ± 0.02). Thus, this extract is suggested to have dual properties of anti-inflammatory and anti-acetylcholinesterase inhibition activity that could be beneficial in the treatment and prevention of neurodegenerative disease.

     

     

     

  • Keywords


    Apple vinegar, date vinegar, Centella asiatica, acetylcholinesterase inhibition, anti-inflammation

  • References


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Article ID: 27510
 
DOI: 10.14419/ijet.v7i4.14.27510




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