Comparative Study on Anti-Acetylcholinesterase and Anti-Inflammatory Activities of Date and Apple Vinegars Fortified with Centella Asiatica

  • Authors

    • M I Adenan
    • N A Jusril
    • K A Radzun
    • Z Z Hafiz
    2019-12-24
    https://doi.org/10.14419/ijet.v7i4.14.27510
  • Apple vinegar, date vinegar, Centella asiatica, acetylcholinesterase inhibition, anti-inflammation

  • Neuroinflammation and deficiency of cholinergic are major factors of neurodegenerative damage correlated to cognitive impairment in Alzheimer’s disease (AD). We investigated the anti-inflammatory and anti-acetylcholinesterase activities of apple and date vinegars added with Centella asiatica in SH-SY5Y neuroblastoma cells. The neuroprotective effect of apple or date vinegar added with various percentage of C. asiatica (0, 0.5, 2, 5) % w/v was determined in vitro. The methanolic extract of apple vinegar added with 2% C. asiatica (AV-2% CA) and date vinegar added with 2% C. asiatica (DV-2% CA)  extracts showed potent neuroprotective effect. Both extracts were subjected to liquid-liquid partitioning yielded aqueous (H2O: AV/DV-2% CA) and ethyl acetate (EA: AV/DV-2% CA) extracts. Anti-inflammatory response against nitric oxide (NO) of all extracts was measured in LPS-induced SH-SY5Y neuroblastoma cells and percentage inhibition of acetylcholinesterase (AChE) was measured using commercially available test kits. It was found that EA: DV-2% CA showed potent ameliorating effect against LPS-induced inflammation (I50: 563.5 ± 0.13μg/mL) and was also responsible in the AChE inhibition activity (IC50: 9.087 ± 0.02). Thus, this extract is suggested to have dual properties of anti-inflammatory and anti-acetylcholinesterase inhibition activity that could be beneficial in the treatment and prevention of neurodegenerative disease.

     

     

     

  • References

    1. [1] J. Park, S. Shin, K. C. Park, E. Jeong, and J. H. Park, “Synthesis and in vitro Assay of New Triazole Linked Decursinol Derivatives Showing Inhibitory Activity against Cholinesterase for Alzheimer’s Disease Therapeutics,†J. Korean Chem. Soc., vol. 60, no. 2, pp. 125–130, 2016.

      [2] A. H. Nour et al., “In vitro Anti-Acetylcholinesterase and Antioxidant Activity of Selected Malaysian Plants,†Asian J. Pharm. Clin. Res., vol. 7, no. 3, pp. 93–97, 2014.

      [3] J. J. J. Zhang et al., “Wound Healing Activities of Different Extracts of Centella asiatica in Incision and Burn Wound Models: An Experimental Animal Study.,†BMC Complement. Altern. Med., vol. 3, no. 1, pp. 8–14, 2013.

      [4] A. Prasad, M. Singh, N. P. Yadav, A. K. Mathur, and A. Mathur, “Molecular, Chemical and Biological Stability of Plants Derived From Artificial Seeds of Centella asiatica (L.) Urban-An Industrially Important Medicinal Herb,†Ind. Crops Prod., vol. 60, pp. 205–211, 2014.

      [5] A. Mas, M. J. Torija, G. Carmne, A-Parrilla, and T. M. Ana, “Acetic Acid Bacteria and the Production and Quality of Wine Vinegar, Acetic Acid Bacteria and the Production and Quality of Wine Vinegar,†Sci. World J., vol. 2014, pp. 1–6, 2014.

      [6] S. Pourmozaffar, A. Hajimoradloo, and H. K. Miandare, “Dietary effect of apple cider vinegar and propionic acid on immune related transcriptional responses and growth performance in white shrimp, Litopenaeus vannamei,†Fish Shellfish Immunol., vol. 60, pp. 65–71, 2017.

      [7] M. H. Matloob, A. Alaa, and A. B. D. A. Balakit, “Phenolic Content of Various Date Palms Fruits and Vinegars From Iraq,†vol. 14, no. 4, pp. 1893–1906, 2016.

      [8] J. Popp et al., “Markers of Neuroinflammation Associated with Alzheimer’s Disease Pathology in Older Adults,†Brain. Behav. Immun., vol. 62, no. 62, pp. 203–211, 2017.

      [9] M. Rahimifard et al., “Targeting the TLR4 Signaling Pathway by Polyphenols: A Novel Therapeutic Strategy for Neuroinflammation,†Ageing Res. Rev., vol. 36, no. 6, pp. 11–19, 2017.

      [10] W. Y. Kwon et al., “Quality characteristics and biological activities of vinegars added with young leaves of Akebia quinata,†J. Korean Soc. Food Sci. Nutr., vol. 43, no. 7, pp. 989–998, 2014.

      [11] H. J. Heusinkveld and R. H. S. Westerink, “Comparison of different in vitro cell models for the assessment of pesticide-induced dopaminergic neurotoxicity,†Toxicol. Vitr., vol. 45, no. July, pp. 81–88, 2017.

      [12] K. Khwanraj, C. Phruksaniyom, S. Madlah, and P. Dharmasaroja, “Differential Expression of Tyrosine Hydroxylase Protein and Apoptosis-Related Genes in Differentiated and Undifferentiated SH-SY5Y Neuroblastoma Cells Treated with MPP+,†Neurol. Res. Int., vol. 2015, 2015.

      [13] G. Sala et al., “Rotenone upregulates alpha-synuclein and myocyte enhancer factor 2D independently from lysosomal degradation inhibition,†Biomed Res. Int., vol. 2013, no. March 2014, 2013.

      [14] M. Ramkumar et al., “Neuroprotective effect of Demethoxycurcumin, a natural derivative of Curcumin on rotenone induced neurotoxicity in SH-SY 5Y Neuroblastoma cells,†BMC Complement. Altern. Med., vol. 17, no. 1, pp. 1–11, 2017.

      [15] Y. Gao et al., “Anti-Inflammatory Effects of Sophocarpine in LPS-Induced RAW 264.7 Cells Via NF-kB and MAPKs Signaling Pathways,†Toxicol. Vitr., vol. 26, no. 1, pp. 1–6, 2012.

      [16] S.-K. Heo, H.-J. Yun, E.-K. Noh, W.-H. Park, and S.-D. Park, “LPS induces inflammatory responses in human aortic vascular smooth muscle cells via Toll-like receptor 4 expression and nitric oxide production,†Immunol. Lett., vol. 120, no. 1, pp. 57–64, 2008.

      [17] S.-H. Lu et al., “The discovery of potential acetylcholinesterase inhibitors: a combination of pharmacophore modeling, virtual screening, and molecular docking studies.,†J. Biomed. Sci., vol. 18, no. 1, p. 8, 2011.

      [18] H. Saleem et al., “In vitro acetylcholinesterase and butyrylcholinesterase inhibitory potentials of Jatropha gossypifolia plant extracts,†Acta Pol. Pharm. - Drug Res., vol. 73, no. 2, pp. 419–423, 2016.

  • Downloads

  • How to Cite

    I Adenan, M., A Jusril, N., A Radzun, K., & Z Hafiz, Z. (2019). Comparative Study on Anti-Acetylcholinesterase and Anti-Inflammatory Activities of Date and Apple Vinegars Fortified with Centella Asiatica. International Journal of Engineering & Technology, 7(4.14), 116-120. https://doi.org/10.14419/ijet.v7i4.14.27510