Biotransformation of Furaltadone, Furazolidone and Nitrofurazone using Aspergillus Tamarii isolate TN-7 : in Vitro Residual Identification and Quantification by HPLC-DAD
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2019-12-24 https://doi.org/10.14419/ijet.v7i4.14.27511
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Aspergillus tamarii isolate TN-7, biotransformation, degradation, HPLC-DAD, nitrofuran. -
Abstract
Nitrofurans (NFs) such as furaltadone (FTD), furazolidone (FZD) and nitrofurazone (NFZ) have been used as antibacterials and growth promoters for the poultry and aquaculture industry. These antibiotics have now been banned from use due to their carcinogenic properties; therefore there is an urgent need to remove or degrade NFs from contaminated areas. Aspergillus tamarii isolate TN-7 isolated from antibiotic overexposed soil shows an ability to degrade the NFs antibiotics. After 5 days of incubating of Aspergillus tamarii isolate TN-7 with 500 µg/mL NF, the residual of the NF concentration was determined by High-Performance Liquid Chromatography-diode array detector (HPLC-DAD). Solid phase extraction was performed to clean-up the fermentation broth prior to HPLC-DAD analysis.  Antimicrobial of the NFs residues showed a decreased in the percentage of inhibition that FTD, FZD and NFZ were reduced to to 85.71 %, 75.86 % and 70.97 % after 96 hours of incubation. Quantification using HPLC-DAD showed, after 96 hours of incubation, Aspergillus tamarii isolate TN-7 reduced furaltadone, furazolidone and nitrofurazone to 86.73 %, 37.49 % and 29.17 % respectively. This finding shows that Aspergillus tamarii isolate TN-7 has the potential to be used as a bioremediation tool in removing NF antibiotics from the contaminated areas. Â
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References
[1] Leston S, Nunes M, Viegas I, Lemos MFL, Freitas A, Barbosa J, Ramos F, & Pardal MA, (2011), The effects of the nitrofuran furaltadone on Ulva lactuca. Chemosphere, 82(7), 1010-1016.
[2] Commission Regulation, Annexes I, II, III and IV to Regulation (EEC) No 2377/90 laying down a community procedure for the establishment of maximum residue limits of veterinary medicinal products in food stuffs of animal origin, C Regulation, et al., Editors. 1995, Official J. Eur. Communities.
[3] Yu WH, Chin TS, & Lai HT, (2013), Detection of nitrofurans and their metabolites in pond water and sediments by liquid chromatography (LC)-photodiode array detection and LC-ion spray tandem mass spectrometry. International Biodeterioration & Biodegradation, 85, 517-526.
[4] Senwan MS, Safian MF, Noor ZM, & Ariffin ZZ, (2017), Isolation and characterization of filamentous fungi from animal agricultural farm soil. Pertanika journal of science and technology, 25, 19-28.
[5] Silambarasan S & Abraham J, (2013), Mycoremediation of Endosulfan and Its Metabolites in Aqueous Medium and Soil by Botryosphaeria laricina JAS6 and Aspergillus tamarii JAS9. PLoS ONE, 8(10).
[6] Ghosh A, Dastidar MG, & Sreekrishnan TR, (2017), Bioremediation of chromium complex dyes and treatment of sludge generated during the process. International Biodeterioration & Biodegradation, 119(Supplement C), 448-460.
[7] Korabecna M, (2007), The variability in the fungal ribosomal DNA (ITS1, ITS2, and 5.8 S rRNA gene): its biological meaning and application in medical mycology. Communicating current research and educational topics and trends in applied microbiology, 2, 783-787.
[8] Dong T, Wu GW, Wang XN, Gao JM, Chen JG, & Lee SS, (2010), Microbiological transformation of diosgenin by resting cells of filamentous fungus Cunninghamella echinulata CGMCC 3.2716. J. Mol. Catal B: Enzym, 67(3-4), 251-256.
[9] Haller MY, Müller SR, McArdell CS, Alder AC, & Suter MJF, (2002), Quantification of veterinary antibiotics (sulfonamides and trimethoprim) in animal manure by liquid chromatography–mass spectrometry. Journal of Chromatography A, 952(1-2), 111-120.
[10] Jewell KS, Castronovo S, Wick A, Falås P, Joss A, & Ternes TA, (2016), New insights into the transformation of trimethoprim during biological wastewater treatment. Water Research, 88, 550-557.
[11] Perez C, Agnese AM, & Cabrera JL, (1999), The essential oil of Senecia graveolens (composite): Chemical composition and antimicrobial activity tests. Journal of Ethnopharmacology, 66(11), 91-96.
[12] Jrah H, Kouidhi B, Flamimi G, & Bakhrouf AM, T., (2011), Chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of Tunisian Nigella sativa essential oil and thymoquinone. Food Chemistry, 129(4), 1469-1474.
[13] Wang JR & Zhang LY, (2006), Simultaneous determination and identification of furazolidone, furaltadone, nitrofurazone, and nitrovin in feeds by HPLC and LC-MS. Journal of liquid chromatography & related technologies, 29(377-390).
[14] Levison ME & Levison JH, (2009), Pharmacokinetics and pharmacodynamics of antibacterial agents. Infectious disease clinics of north America, 23(4), 791-815.
[15] Dariush N, (2008), Effects of aeration, agitation and pH on the production of mycelial biomass and exopolysaccharide from the filamentous fungus Ganoderma lucidum. Dynamic Biochemistry, Process Biotechnology and Molecular Biology, 2(1), 14-18.
[16] Technologies A, Using DMSO as injection solvent to increase sample load in preparative LC. 2005: USA.
[17] Nouws JFM, Vree TB, Aerts MML, Degen M, & Driessens F, (1987), Some pharmacokinetic data about furaltadone and nitrofurazone administered orally to preruminant calves. Veterinary Quarterly, 9(3), 208-214.
[18] European Food Safety Authority E, (2015), Scientific Opinion on nitrofurans and their metabolites in food. European Food Safety Authority Journal, 13(6), 4140.
[19] Boelsterli UA, Ho HK, Zhou S, & Leow KY, (2006), Bioactivation and hepatotoxicity of nitroaromatic drugs. Current Drug Metabolism, 7, 715-727.
[20] Aracena P, Lazo-Hernandez. C., Molina-Berrios A, Sepulveda DR, Reinoso C, Larrain JI, Navarro J, & Letelier ME, (2014), Microsomal oxidative stress induced by NADPH is inhibited by nitrofurantoin redox biotransformation. Free Radical Research, 48, 129-136.
[21] Belinda SH, Christopher B, & Shane RW, (2011), Nifurtimox activation by Trypanosomal Type I Nitroreductases generates cytotoxic nitrile metabolites. Journal of Biological Chemistry 286(15), 13088-13095.
[22] Ertan G, Karasulu Y, & Güneri T, (1993), Degradation and gastrointestinal stability of nitrofurantoin in acidic and alkaline media. International Journal of Pharmaceutics, 96(1), 243-248.
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How to Cite
S Mohammad, N., I A Halim, M., M Mahat, M., F Safian, M., & Z Ariffin, Z. (2019). Biotransformation of Furaltadone, Furazolidone and Nitrofurazone using Aspergillus Tamarii isolate TN-7 : in Vitro Residual Identification and Quantification by HPLC-DAD. International Journal of Engineering & Technology, 7(4.14), 121-126. https://doi.org/10.14419/ijet.v7i4.14.27511Received date: 2019-02-18
Accepted date: 2019-02-18
Published date: 2019-12-24