Collagen-Chitosan Hydrogel Formed in Situ Via Enzymatic Crosslinking

  • Authors

    • Lau Sin Mun
    • Masrina Mohd Nadzir
    • Shiplu Roy Chowdhury
    • Mohd Fauzi Mh Busra
    2018-12-03
    https://doi.org/10.14419/ijet.v7i4.38.27885
  • In situ hydrogel, composite, gelation, collagen, chitosan

  • Abstract

    In this research, injectable collagen-chitosan conjugates (Col-Chit-Ph) hydrogel was formed by oxidative coupling of phenolic hydroxyl (Ph) groups in polymer chain via peroxidase-catalysed crosslinking reaction. The resulting Col-Chit-Ph solutions formed rapid hydrogel at physiological conditions by utilizing H2O2. The amount of chitosan in the compositions of Col-Chit-Ph hydrogels influenced the mechanical properties of the hydrogels. Here, conjugates having Col:Chit ratio of 1:1 showed greater resistance to compression compared to conjugates having Col:Chit ratio of 3:2. Meanwhile, the efficiency of cell attachment for conjugates with Col:Chit ratio of 4:1 is higher than conjugates with Col:Chit ratio of 1:1. Although the biological properties of hydrogel was good at Col:Chit ratio of 4:1, this hydrogel easily destroyed by external force. However, with higher amount of chitosan in the composite, hydrogels were more mechanically stable. This indicates the importance of the chitosan ratio to the novel Col-Chit-Ph injectable hydrogel composite.

     

     


     

  • References

    1. [1] El-Sherbiny, I. M., & Yacoub, M. H., 2013. Hydrogel scaffolds for tissue engineering: Progress and challenges. Global Cardiology Science and Practice, 38: 316-342.

      [2] Jiang, Y., Wang, H., Deng, M., Wang, Z., Zhang, J., Wang, H., & Zhang, H., 2016. Effect of ultrasonication on the fibril-formation and gel properties of collagen from grass carp skin. Materials Science and Engineering: C, 59: 1038-1046.

      [3] Jin, R., Hiemstra, C., Zhong, Z., & Feijen, J., 2007. Enzyme-mediated fast in situ formation of hydrogels from dextran–tyramine conjugates. Biomaterials, 28(18): 2791-2800.

      [4] Le Thi, P., Lee, Y., Nguyen, D. H., & Park, K. D., 2017. In situ forming gelatin hydrogels by dual-enzymatic cross-linking for enhanced tissue adhesiveness. Journal of Materials Chemistry B, 5(4): 757-764.

      [5] Lee, F., Chung, J. E., & Kurisawa, M., 2008. An injectable enzymatically crosslinked hyaluronic acid–tyramine hydrogel system with independent tuning of mechanical strength and gelation rate. Soft Matter, 4(4): 880-887.

      [6] Sakai, S., Yamada, Y., Zenke, T., & Kawakami, K., 2009. Novel chitosan derivative soluble at neutral pH and in situ gellable via peroxidase-catalyzed enzymatic reaction. Journal of Materials Chemistry, 19(2): 230-235.

      [7] Sionkowska, A., Wisniewski, M., Skopinska, J., Kennedy, C. J., & Wess, T. J., 2004. Molecular interactions in collagen and chitosan blends. Biomaterials, 25(5): 795-801.

      [8] Tahrir, F. G., Ganji, F., & M Ahooyi, T., 2015. Injectable thermosensitive chitosan/glycerophosphate-based hydrogels for tissue engineering and drug delivery applications: a review. Recent patents on drug delivery & formulation, 9(2): 107-120.

      [9] Wang, X., Sang, L., Luo, D., & Li, X., 2011. From collagen–chitosan blends to three-dimensional scaffolds: the influences of chitosan on collagen nanofibrillar structure and mechanical property. Colloids and Surfaces B: Biointerfaces, 82(1): 233-240.

      [10] Yunoki, S., & Matsuda, T., 2008. Simultaneous processing of fibril formation and cross-linking improves mechanical properties of collagen. Biomacromolecules, 9(3): 879-885.

  • Downloads

  • How to Cite

    Sin Mun, L., Mohd Nadzir, M., Roy Chowdhury, S., & Fauzi Mh Busra, M. (2018). Collagen-Chitosan Hydrogel Formed in Situ Via Enzymatic Crosslinking. International Journal of Engineering & Technology, 7(4.38), 1402-1404. https://doi.org/10.14419/ijet.v7i4.38.27885

    Received date: 2019-02-24

    Accepted date: 2019-02-24

    Published date: 2018-12-03