Investigation of Pulse Electric Field Effect on HT29 Cell Alignment Properties Cultured on Laminin Micro-Patterned Surface

  • Authors

    • Hassan Buhari Mamman
    • Muhammad Mahadi Abdul Jamil
    • Mohammed Ahmed Bawa
    • Mohamad Nazib Adon
    2018-05-06
    https://doi.org/10.14419/ijet.v7i3.36.29088
  • Cell membrane, cell migration, electric field, wound healing.
  • Pulse electric field (PEF) is a way of generating transient holes in the cell membrane. This is achieved by exposing the cell to a high voltage electric field of usually of short duration. The application of PEF to the cell cannot only open pores in the cell membrane but can also affect the cell physiology. Extracellular matrix protein is the major regulator of many cellular functions such as proliferation, adhesion and migration. PEF was also found to modulate these cellular behaviours. However, a combined influence of PEF and ECM on cellular behaviour which could further enhances the cellular processes for wound healing application via directed cell migration has not been investigated. Therefore, the aim of this study is to examine the effect of PEF in combination with ECM on the cell guidance and self-assemble monolayer of HT29 cell line. Cell alignment was investigated via micro-contact printing techniques. The results of the study have shown that PEF has improved the HT29 cell alignment and elongation by more than 40%. Since tissue development in multicellular organisms in the course of wound healing depends on the cell adhesion process which can be influence by electrostatic charges. Therefore, manipulation of substrate charge by patterning the substrate and application of PEF to enhance cell adhesion is a promising scheme that can regulate cell guidance for wound healing application.

     

     

     

  • References

    1. [1] Raab, M., Daxecker, H., Edwards, R. J., Treumann, A., Murphy, D. & Moran, N., “Protein interactions with the platelet integrin αIIb regulatory motifâ€, Proteomics, Vol.10, No.15, (2010), pp. 2790-2800.

      [2] Metcalfe, A. D. & Ferguson, M. W., “Tissue engineering of replacement skin: the crossroads of biomaterials, wound healing, embryonic development, stem cells and regenerationâ€, Journal of the Royal Society Interface, Vol.4, No.14, (2007), pp. 413-437.

      [3] Han, F., Gilbert, J. R., Harrison, G., Adams, C. S., Freeman, T., Tao, Z. & Norton, P. A., “Transforming growth factor-β1 regulates fibronectin isoform expression and splicing factor SRp40 expression during ATDC5 chondrogenic maturationâ€, Experimental Cell Research, Vol.313, No.8, (2007), pp. 1518-1532.

      [4] Jamil, M. M. A., Youseffi, M., Britland, S. T., Liu, S., See, C. W., Somekh, M. G. & Denyer, M. C. T., “Widefield Surface Plasmon Resonance Microscope: a Novel Biosensor Study of Cell Attachment to Micropatterned Substrates†In 3rd Kuala Lumpur International Conference on Biomedical Engineering, (2007), pp. 334-337.

      [5] Perl, A., Reinhoudt, D. N. & Huskens, J., “Microcontact printing: limitations and achievementsâ€, Advanced Materials, Vol.21, No.22, (2009), pp. 2257-2268.

      [6] Mamman, H. B., Jamil, M. M. A., & Adon, M. N., “Studying the influence of electroporation on HT29 cell line interaction with micro-patterned extracellular matrix protein (fibronectin)â€, In Control System, Computing and Engineering (ICCSCE), (2016), pp. 292-297.

      [7] Mamman, H. B., Jamil, M. M. A. & Adon, M. N., “Investigation of Electroporation Effect on HT29 Proliferation Rate and Cell Size Plated on Fibronectin Coated Substrateâ€, Advance Science Letter, Vol.24, No.6, (2018), pp4387-4390.

      [8] Sefat, F., Denyer, M. C. T. & Youseffi, M., “Imaging via widefield surface plasmon resonance microscope for studying bone cell interactions with micropatterned ECM proteinsâ€, Journal of Microscopy, Vol.241, No.3, (2011), pp. 282-290.

      [9] Rahman, N. A. A., Mamman H. B. & Jamil, M. M. A., “An overview: Investigation of Electroporation technique on cell properties culture on the micro-patterned surfaceâ€, Jurnal Teknologi, Vol.77, No.6, (2015), pp 61-65

      [10] Mamman, H. B., Jamil, M. M. A. & Adon, M. N., “Optimization of electric field parameters for HT29 cell line towards wound healing applicationâ€, Indian Journal of science and Technology, Vol.9, No.46, (2016), pp1-7

      [11] Khaghani, S., Sefat, F., Denyer, M., & Youseffi, M., “Alignment of rat primary chondrocyte cells to collagen type-i, fibronectin and lamininâ€, Journal of Anatomy, Vol.213, No.3, (2008), pp. 351.

      [12] Bradshaw, M., Ho, D., Fear, M. W., Gelain, F., Wood, F. M. & Iyer, K. S., “Designer self-assembling hydrogel scaffolds can impact skin cell proliferation and migrationâ€, Scientific Reports, Vol.4, (2014), pp. 1-6.

      [13] Iwai, R., Nemoto, Y. & Nakayama, Y. “The effect of electrically charged polyion complex nanoparticle-coated surfaces on adipose-derived stromal progenitor cell behaviorâ€, Biomaterials, Vol.34, No.36, (2013), pp. 9096-9102.

      [14] Gumbiner, B. M., “Regulation of Cadherin-mediated Adhesion in Morphogenesisâ€, Nature Reviews Molecular Cell Biology, Vol.6, No.8, (2005), pp. 622-634.

      [15] Théry, M. “Micropatterning as a tool to decipher cell morphogenesis and functionsâ€, Journal of Cell Science, Vol.123, No.24, (2010), pp. 4201-4213.

      [16] Sefat, F., Youseffi, M., Khaghani, S. A., Soon, C. F. & Javid, F., “Effect of Transforming Growth Factor-β3 on mono and multilayer chondrocytesâ€, Cytokine, Vol.83, (2016), pp. 118-126.

      [17] Funk, R. H. & Monsees, T. K., “Effects of electromagnetic fields on cells: physiological and therapeutical approaches and molecular mechanisms of interactionâ€, Cells Tissues Organs, Vol.182, No.2, (2006), pp. 59-78.

      Taghian, T., Sheikh, A. Q., Kogan, A. B., & Narmoneva D., “Harnessing electricity in biosystems - A functional tool for tissue engineering applicationsâ€, Austin Journal of Biomedical Engineering, Vol.1, No.5, (2014), pp. 1-5
  • Downloads

  • How to Cite

    Buhari Mamman, H., Mahadi Abdul Jamil, M., Ahmed Bawa, M., & Nazib Adon, M. (2018). Investigation of Pulse Electric Field Effect on HT29 Cell Alignment Properties Cultured on Laminin Micro-Patterned Surface. International Journal of Engineering & Technology, 7(3.36), 108-112. https://doi.org/10.14419/ijet.v7i3.36.29088