Phytochemistry and antibacterial activity of the methanol, petroleum ether and aqueous extracts of the leaves, stem bark and roots of bobgunnia fistuloides

 
 
 
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  • Abstract


    Ethno-medicinally, Bobgunnia fistuloides parts (leaves, bark and roots) have been used by the Nupe communities in Niger State, Nigeria to treat various diseases for decades. The phytochemical and antibacterial activity of the methanol, petroleum ether and aqueous extracts of the leaves, roots and stem bark of Bobgunnia fistuloides were investigated using standard methods. The leaves, roots and stem bark of Bobgunnia fistuloides plant were collected from Binin village, Gbako Local Government Area (LGA) in Niger State Nigeria. The antibacterial activities of the plant extracts were determined using 5 pathogenic bacteria (Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoneae, Salmonella typhi and Escherichia coli). The result of the phytochemicals, in mg/100 g, revealed the values of 479.73±0.20, 465.98±0.14, 482.77±0.17; 1.71±0.06, 25.14±0.02, 23.90±0.03; 29.09±0.20, 36.77±0.06, 24.79±0.14; 22.40±0.02, 25.14±0.02, 23.90±0.03; 577.29±0.24, 586.52±0.11, 230.19±0.03 respectively for the phenols, flavonoids, alkaloids, tannins and saponnins contents of the roots; leaves and stem bark of the plant. In this study, the saponnins and phenol contents of the plant parts were relatively higher than the other parameters investigated. The leaves were found to contain more active principles, followed by the roots and stem bark. Based on the antibacterial activities, the result revealed that the respective zones of inhibition of the growths of the test microorganisms, with the exception of the aqueous leaves extract (against Staphylococci aureus; 25.00±1.00 mm) recorded highest values for the petroleum ether extracts of the stem bark (32.67±0.58, 33.00±1.00, 31,67±0.58 and 35.10±1.00 mm for E. coli, B. subtilis, S. typhii and K. pneumonea respectively). Statistically, Klebsiella pneumoneae (17.13±0.00 mm) recorded the highest average zones of inhibition based on the susceptibility of the pathogens to the extracts, followed by Salmonella typhi (16.92±1.00mm). The least zone of inhibition (12.00±0.50 mm) was recorded for B. subtilis using the stem bark aqueous extract of the plant while the highest value was recorded for the petroleum stem bark extract against K. pneumonea (35.00±1.00 mm). The MIC and MBC of the extracts ranged from 12.50 to 100.00 mg/cm3 respectively. The phytochemical components and antibacterial activities of the plant extracts in this study support the therapeutic value and the ethno-medicinal applications of this plant by the Nupe communities of Niger State Nigeria.

     

     

     


  • Keywords


    Antibacterial; Bioactive Components; Bobgunnia fistuloides; Inhibitors; Phytochemicals.

  • References


      [1] Achi NK, Onyeabo C, Ekeleme-Egedigwe CA & Onyeanula JC (2017), Phytochemical, Proximate Analysis, Vitamin and Mineral Composition of Aqueous Extract of Ficus capensis leaves in South Eastern Nigeria. Journal of Applied Pharmaceutical Science, 7 (3):117-122.

      [2] Adelanwa EB, Ola-Bello, AO &Sakariyahu SK (2016), Comparative studies on phytochemicals screening and antimicrobial activities of shed leaves, fresh leaves and roots of Eucalyptus Camaldulensis in Zaria, Nigeria. Annals of Experimental Biology, 4 (2): 16-22.

      [3] Alexander P (2016), Phytochemical Screening and Mineral Composition of the Leaves of Ocimum gratissimum (Scent Leaf). International Journal of Applied Science and Biotechnology, 4(2): 161-165. https://doi.org/10.3126/ijasbt.v4i2.15101.

      [4] Alfonso VA &Miller RB (2002), Wood Anatomy of the Brazilian Species of Swartzia and Considerations Within the Tribe Swartzieae .IAWA Journal, 23(4): 359-390 https://doi.org/10.1163/22941932-90000310.

      [5] Anwani SE, Etsuyankpa MB & Ogah SPI (2020), Assessment of the proximate and Elemental Composition of the Leaves, Stem bark and Roots of Bobgunnia fistuloides. World Journal of Applied Chemistry, 5(4): 57-64.

      [6] AOAC (2005). Official Methods of Analysis. 18th edn, Association of Official Analytical Chemists, Washington D.C p. 106.

      [7] Arpita R (2017), A Review on the Alkaloids an Important Therapeutic Compound from Plants. International Journal of Plant Biotechnology, 3(2): 1-9. www.journalspub.com

      [8] Bolanle AO, Funmilola AS & Adedayo A (2014), Proximate Analysis, Mineral Contents, Amino Acid Composition, Anti-Nutrients and Phytochemical Screening of Brachystegia Eurycoma Harms and Pipper guineense Schum and Thonn. American Journal of Food and Nutrition, 2(1): 11-17.

      [9] Chaghaby GAE, Ahamd, AF &Ramis S (2014), Evaluation of the antioxidant and antibacterial properties of various solvents extracts of AnnonasquamosaL. leaves. Arabian Journal of Chemistry, 7(2): 227-233. https://doi.org/10.1016/j.arabjc.2011.06.019.

      [10] Chang C, Yang M, Wen H &Chern J (2002), Estimation of total flavonoid content in Propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10:178-182.https://doi.org/10.38212/2224-6614.2748.

      [11] Cragg, GM & David JN (2001), Natural Product Drug Discovery in the Next Millennium. Pharmaceutical Biology, 39:8-17.https://doi.org/10.1076/phbi.39.s1.8.0009.

      [12] Deepak K, Rupali S & Mahesh K (2016), An Over Review of Major Classes of Phytochemicals: Their Types and Role in Disease Prevention. Hislopia Journal, 9 (1/2):1-11.

      [13] Derong L, Mengshi X, Jingjing Z, Zhuohao L, Baoshan X, Xindan L, Maozhu K, Liangyu L, Qing Z, Yaowen L, Hong C, Wen Q, Hejun W &Saiyan C (2016), An Overview of Plant Phenolic Compounds and their Importance in Human Nutrition and Management of Type 2 Diabetes.Molecules, 21(1374): 1-19.https://doi.org/10.3390/molecules21101374.

      [14] Dutta AC (2003), Botany for Degree Students. 6th Edn., Oxford University Press, New Delhi, India, pp: 301-588.

      [15] Egbuna C & Ifemeje JC (2015), Biological Functions and Anti-nutritional Effects of Phytochemicals in Living System. IOSR Journal of Pharmacy and Biological Sciences, 10(2): 10-19.

      [16] Ejikeme CM, Ezeonu CS & Eboatu AN (2014 ), Determination of physical and phytochemical constituents of some tropical timbers indigenous to Niger Delta Area of Nigeria. European Scientific Journal, 10(18): 247–270.

      [17] Etonihu AC, Lawal AT &Etonihu JC (2011), Phytochemical, Antimicrobial and Antimalaria Analysis of Citrus medica Leaves and Perinari polyandara Stem Bark. NSUK Journal of Science and Technology, 1(1):118-124.

      [18] Gnanaraja R, Veeru P, Shanta P & Mahendraverman M (2014), Qualitative and quantitative Phytochemical Analysis of Selected Fabaceae Medicinal Plants from Allahabad region. The Pharma Innovation Journal, 3(7): 53-56.

      [19] Groom A (2012). Bobgunnia fistuloides. The IUCN Red List of Threatened Species.

      [20] Han X, Shen T & Lou H (2007), Dietary Polyphenols and their Biological Significance. International Journal of Molecular Science, 8:950-988.https://doi.org/10.3390/i8090950.

      [21] Kadiri M, Ojewumi AW & Onatade TN (2015), Indigenous Uses and Phytochemical Contents of Plants Used in the Treatment of Menstrual Disorders and After- Child Birth Problems in Abeokuta South Local Government Area of Ogun State, Nigeria. Journal of Drug Delivery and Therapeutics, 5(3):33-42.https://doi.org/10.22270/jddt.v5i3.1144.

      [22] Kawo AH &Kwa AM (2011). Phytochemical screening and antibacterial activity of the aqueous extracts and fractions of ethanolic extracts of Lawsonia inermis Leaf. International Research Journal of Microbiology, 2(12):510-516.

      [23] Keta JN, Obaroh IO, Singh D, Manga SS, Keta MN, Attah D & Aliyu DG (2018), Nutritive and Proximate Analysis of Some Plants Species in Kebbi State, Nigeria. Equity Journal of Science and Technology, 5(1): 57-67.

      [24] Mahesh B & Satish S (2008), Antimicrobial Activity of Some Important Medicinal Plants Against Plant and Human Pathogens. World Journal of Agricultural Science, 4(5): 839-843.

      [25] Misra A (2009), Studies on Biochemical and Physiological Aspects in Relation to Phyto-Medicinal Qualities and Efficacy of the Active Ingredients during the Handling, Cultivation and Harvesting of the Medicinal Plants. Journal Medicinal Plants Research, 3, 1140-1146.

      [26] Murnisyazwani J & Rabeta MS (2019), Antioxidant and antimicrobial activity of sapodilla (Manilkara zapota L.) fresh, juice and bar. Food Research, 3(5): 400 – 406.https://doi.org/10.26656/fr.2017.3(5).085.A.

      [27] Nas FS, Oyeyi TI & Ali M (2018), Antibacterial efficacy and phytochemical screening of Senna siamea leaves extracts on some pathogenic bacteria. Journal of Microbiology & Experimentation, 6(3) :159-163.https://doi.org/10.15406/jmen.2018.06.00208.

      [28] Oloyed OI (2005), Chemical profile of unripe pulp of Carica papaya. Pakistan Journal of Nutrition, 4, 379-381.https://doi.org/10.3923/pjn.2005.379.381.

      [29] Osuntokun OT (2018), Evaluation of Inhibitory Zone Diameter (IZD), Phytochemical Screening, Elemental Composition and Proximate Analysis of Crude Cleistopholis Patens (Benth) on Infectious Clinical Isolates. Journal of Molecular Biomarkers and Diagnosis, 9(2):1-5.https://doi.org/10.4172/2155-9929.1000385.

      [30] Oyen, LPA. (2012). Bobgunnia fistuloides (Harms) J.H.Kirkbr&Wiersema. [Internet] Record from PROTA4U.Lemmens, R.H.M.J., Louppe, D. & Oteng-Amoako, A.A. (Editors).PROTA (Plant Resources of Tropical Africa / Ressourcesvégétales de l’Afriquetropicale), Wageningen, Netherlands. http://www.prota4u.org/search. asp.

      [31] Panda SK & Dutta SK (2011), Antibacterial Activity from Bark Extracts of Pterospermum acerifolium (L.) Willd. International Journal of Pharmaceutical Science and Research, 2(3): 584-595.

      [32] Patil UH & Gaikwad UK (2011) Phytochemical evaluation and Bactericidal potential of Terminalia arjuna stem bark. International Journal of Pharmaceutical Science and Research, 2(3):614-619.

      [33] Sani MH, Garba I, Umar HD, Zakariya AM & Muhammad K (2016), Phytochemical Study and Antibacterial Properties of the Leaf Extracts of Swartzia madagascariensisDesv (Fabaceae). British Microbiology Research Journal, 11(6): 1-6.https://doi.org/10.9734/BMRJ/2016/22372.

      [34] Shuruq A, Noura A, Ghada A, Ghadeer A, Kavita MS, Maha AT &Nesrine AH (2017), Role of Phytochemicals in Health and Nutrition. BAOJ Nutrition, 3: 028. https://bioaccent.org/nutrition/nutrition28.php

      [35] Singleton VL, Orthofer R &Lamuela-Raventos RM (1999), Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178.https://doi.org/10.1016/S0076-6879(99)99017-1.

      [36] Sofowora, A. (2008), Medicinal Plants and Traditional medicine in Africa. 3rd ed. Spectrum Books limited, Ibadan, Nigeria, pp. 37-43, 199-204, and 249-258.

      [37] Spencer JPE (2008), Flavonoids: Modulators of Brain Function? British Journal of Nutrition, 99: 60–77. https://doi.org/10.1017/S0007114508965776.

      [38] Thatoi H, Panda SK &Dutta SK (2008), Phytochemical and Antimicrobial Evaluation of three Medicinal Plants from Similipal: A note. Plant Science Research, 30, 48-51.

      [39] Tsobou R, Pierre-Marie M, Voukeng KI, & Van DP (2015), phytochemical screening and antibacterial activity of medicinal plants used to treat typhoid fever in Bamboutos division, West Cameroon. Journal of Applied Pharmaceutical Science, 5(6): 034-049.https://doi.org/10.7324/JAPS.2015.50606.


 

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Article ID: 31394
 
DOI: 10.14419/ijac.v9i1.31394




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