Bioprospective potentials of endophytic fungi penicillium SPP isolated from leaves of azadirachta indica (A. JUSS)

  • Abstract
  • Keywords
  • References
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  • Abstract

    The focus of this research work was to isolates and identify endophytic fungi from young leaves and stems (twig) of Azadirachta indica, family (Meliaceae) for the production and assay of the produced secondary metabolites. Altogether 126 segments were used, of which 63 segments each from leaves and stems (twig) tissues were screened, using modified surface sterilization techniques. A total of 12 species of endophytic fungi were purely isolated, Penicillium spp was randomly selected for the extraction and evaluation of its secondary metabolites. The eluent collected from column chromatography mixture of ethylacetate and n- hexane (50:50v/v) was phytochemically screened, and the results showed the presence of saponins, flavonoids and phenols. Based on disc diffusion method of sensitivity, the eluent possessed some degree of antibacterial and antifungal activities. In addition, in-vitro antioxidant potentiality of the eluent was also evaluated using 2, 2- Diphenyl-1-picrylhydrazyl radical (DPPH, Sigma Aldrich). Statistical package for social science (SPSS 3/93) software was used to analyze the results using one way Analysis of Variance (ANOVA), which revealed no significant difference, on the effect of concentrations of eluent on test organisms but the eluent showed significant difference on scavenging free radicals at a critical value (p >0.05).

  • Keywords

    Antimicrobial Screenin; Antioxidant, Endophytic Fungi; Secondary Metabolites; Surface Sterilization.

  • References

      [1] Ahmad, FB. Williams, PA. Doublier, JL. Durand, S. Buleon, A. (1999) physicochemical characteristics of sago starch. Carbohydrate Polymer, (38): 361-370.

      [2] Anima Nanda and S. Akila (2014). Cytotoxicity Assay of Secondary Metabolites produced from Mould Fungi: Penicillium spp. International Journal of Pharm Tech Research, 6(3):954-958.

      [3] Arechavaleta, M., Bacon, C.W., Hoveland, C.S., Radcliffe D. (1989).Effect of the tall fescue endophytes on plants responds to environmental stress. Agronomy journal. (81): 83-90.

      [4] Barnett, H.L., and B.B. Hunter. (1987). Illustrated Genera of Imperfect Fungi. MacMillan Publication. Co., New York. [5] Breen, J.P., (1994). Acremonium endophyte interactions with enhanced plant resistance to insects. Annual review of entomology. (39): 401-423.

      [5] Brooth, C., (1997). Laboratory guide to the identification of the major species of fungi. Commonwealth mycological institute, Key survey England. Pp 172-181.

      [6] Cheesbrough, M. (2000). District laboratory practice in Tropical countries. Cambridge University press, London. (2):76-100.

      [7] Clarke BB., White Jr., Hurley RH., Torres MS., Sun S., Huff DR., (2006).Endophytes- mediated suppression of dollar spot disease in fine fescues, plant diseases (90): 994-998.

      [8] Devi NN, Prabakaran JJ (2014). Bioactive metabolites from an endophytic fungus Penicillium sp. isolated from Centella asiatica. Current Research in Environmental & Applied Mycolog 4(1), 34–43.

      [9] Dreyfuss, M. M., and Chapela, I. H. (1994). Potential of fungi in the discovery of novel, low- molecular weight pharmaceuticals. In V. P. Gullo (ed.), the discovery of natural products with therapeutic potential. Butter worth-Heinemann, London, U. K.: 49-80.

      [10] European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2012). Disc diffusion method for antimicrobial susceptibility testing. Version 2.1.

      [11] Ge, Hui Ming. Shen, Yao Zhu. Chun Hua, Tan, Shu Hua. Ding, Hui. Song, Yong Chun and Tan, Ren Xiang (2008). Penicidones A-

      [12] C, three cytotoxic alkaloidal metabolites of an endophytic Penicillium sp. Phytochemistry, 69(2): 571-576.

      [13] Harold Benson (1998). .Microbial applications, lab manual in general Microbiology 7th Edition mcb Mcgraw Hill Company.Pp62.

      [14] Kharwar RN, Verma VC, Kumar A, Gond SK, Harper JK, Hess WM, Lobkovosky E, Ma C, Ren Y, Strobel GA. (2009) Javanicin, an antibacterial naphthaquinone from an endophytic fungus of neem, Chloridium sp. Curr Microbiol. 58(3):233-8.

      [15] Kwon, Oh Eok. Rho, Mun-Chual. Song, Hye Young. Lee, Seung Woong. Chung, Mi Yeon. Lee, Jeong Hyun. Kim, Young Ho. Lee, Hyun Sun. Kim, Young-Kook (2002). Phenylpyropene A and B, new inhibitors of acyl-CoA: cholesterol acyltransferase produced by Penicillium griseofulvum F1959. Journal of Antibiotics, 55 (11): 1004-1008.

      [16] Kusari Souvik, Vijay C., Verma, Marc Lamshoeft, Michael Spiteller, (2012). An endophytic fungus from Azadirachta indica A. Juss. That produces azadirachtin. World Journal of Microbiology and Biotechnology, 28 (3): 1287-1294

      [17] Larsen, Thomas Ostenfeld. Lange, Lene. Schnorr, Kirk. Stender, Steen and Frisvad, Jens Christian (2007). Solistatinol, a novel phenolic compactin analogue from Penicillium solitum. Tetrahedron Letters, 48 (7): 1261-1264.

      [18] Lucas, Esther M.F. Castro, Mateus C.M. and Takahashi (2007). Jacqueline Antimicrobial properties of sclerotiorin, iso -chromophilone VI and pencolide, metabolites from Brazilian cerrado isolate of Penicillium sclerotiorum Van Beyma. Brazilian Journal of Microbiology. 38 (4): 785-789.

      [19] National Committee for Clinical Laboratory Standards (NCCLS) (2000). Methods for dilution antimicrobial assays for bacteria that grow aerobically. Approved Standard M7-A5.

      [20] Nicoletti, Rosario, Lopez-gresa, Maria Pilar. Manzo, Emiliano. Carella, Angela and Ciavatta, Maria Letizia (2007). Production and fungitoxic activity of Sch. 642305, a secondary metabolites of Penicillium canescens. Mycopathologia, 163 (5): 295-301.

      [21] Okujagu, T.F., Etatuvie sam O.,Eze Ifenyinwa, Jimoh Bisola, Nwokeke Celina, Mbaoji cammilus, Mohammed Zarma (2004). Medicinal plants of Nigeria South-west Nigeria. Vol.1.

      [22] Olajide, O. Idowu, D. Adebiyi, A. Afolayan Michael, Orishadipe, A. Omojola, M. And Thomas, S. (2012). Phytochemical, Antioxidant and Cytotoxicity Properties of Anchomanes Difformis (Bl.) Engl. Tuber Extract. (8): 173-181.

      [23] Oses, R. Valenzuela, S. Freer, J. Sanfuentes, E. Rodríguez, J. (2008). Fungal endophytes in xylem of healthy Chilean trees and their possible role in early wood decay. Fungal Diversity. 33: 77-86.

      [24] Rancic, Ana. Sokovic, Marina. Karioti, Anastasia. Vukojevic, Jelena and Skaltsa, Helen. (2006). Isolation and structural elucidation of two secondary metabolites from the filamentous fungus Penicillium ochrochloron with antimicrobial activity. Pharmacology, 22 (1): 80-84.

      [25] Raviraja, NS. Maria, GL and Sridhar KR. (2006). Antimicrobial evulation of Endophytic fungi inhabiting medicinal plants of the Western Ghats of India. English Life Sciences. Pp. 6515-6520.

      [26] Rungjindamai, N. Pinruan, U. Choeyklin, R. Hattori, T and Jones

      [27] EBG. (2008). Molecula characterization of basidiomycetous endophytes isolated from leaves, rachis and petioles of oil palm, Elaeis guineensis, in Thailand. Fungal Diversity. 33:139-162. Schardl, C. L., and Philips, T. D. (1997). Protective grass endophytes: Where are they from and where are they going? Plant Disease. (81): 430-437.

      [28] Silva1, M.R.O., Almeida, A.C., Arruda, F.V.F. and Gusmão N. (2011). Endophytic fungi from Brazilian mangrove plant Lagun- cularia racemosa (L.) Gaertn. (Combretaceae): their antimicrobial potential: 1260-1266.

      [29] St-Germain, G. and R. Summer bell. (1996). identifying filamentous fungi: a clinical laboratory handbook. Star Publ. Co., Belmont, California.

      [30] Strobel G. A (2002). Microbial gifts from rain forests. Can., Journal of Plant Pathology. (24):14-20.

      [31] Suryanarayanan, T. S. Venkatesan, G. and Murali, T. S. (2003). Endophytic fungal communities in leaves of tropical forest trees: diversity and distribution. Curr. Sci. 85:489–493.

      [32] Takahashi, Jacqueline, A. and Lucas, Esther M.F. (2008). Occurrence and structural diversity of fungal metabolites with antibiotic activity. Quimica. 31 (7): 1807-1813.

      [33] Theantana, T. Hyde, KD. Lumyong, S. (2009). Aspaginase production by endophytic fungi from Thai medicinal plants: cytotoxic properties. International Journal of Integrative Bio.




Article ID: 6069
DOI: 10.14419/ijbr.v5i1.6069

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