Effect of Annona muricata pre-treatment on liver synthetic ability, kidney function and hematological parameters in dimethylnitrosamine (DMN)-administered rats
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2016-01-19 https://doi.org/10.14419/ijm.v4i1.5709
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Annona Muricata, Anaemia, Dimethylnitrosamine, Liver Synthetic Ability, Rats. -
Abstract
Background:Dimethylnitrosamine (DMN) is a potent hepatotoxin, carcinogen and mutagen which exerts carcinogenic effects and induces hepatic necrosis in experimental animals through metabolic activation by CYP2E1. This study thus evaluated efficacies of Annona muricata pre-treatment on acute dimethylnitrosamine (DMN)-induced anemia and biochemical alterations in male albino rats.
Methods:Four (4) groups of six (6) rats each were used for the study. Group 1 served as control and was untreated, group 2 and 3 were pre-treated with 400mg/kg Annona muricata ethanol leaf extract for one week while group 3 and 4 each received single dose of 20mg/kg DMN (orally) after one week. The rats were sacrificed 48hrs after DMN administration.
Results:In rats administered 20mg/kg DMN, toxicty was clearly shown by decreased activities of serum liver synthetic molecules namely total protein, albumin, increased total bilirubin, increased kidney parameters such as creatinine and urea as well as decreased body weight gain. The toxic effect of DMN was also indicated by significantly decreased levels of red blood cells (RBC), hemoglobin (Hb), packed cell volume (PCV), white blood cells (WBC) and platelet count (Plt count). However, in rats pre-treated with 400mg/kg Annona muricata prior to DMN administration, there were significant reversals in the activities of serum liver synthetic molecules, kidney profiles, and hematological parameters when compared to DMN-alone administered rats.
Conclusion:Annona muricata pre-treatment exhibited in vivo protective and anti-anemic effects against DMN-induced injury.
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References
[1] Al-Qarawi AA, Al-Damegh MA & El-Mougy SA. (2003). Hepatoprotective influence of Adansonia digitata pulp. Journal of Herbs, Spices & medicinal plants 10(3), 1-5.http://dx.doi.org/10.1300/J044v10n03_01.
[2] Adesanoye OA &Farombi EO. (2009). Hepatoprotective effects of Vernonia amygdalina (astereaceae) in rats treated with carbon tetrachloride. Experimental Toxicology and Pathology 62, 197-206.http://dx.doi.org/10.1016/j.etp.2009.05.008.
[3] Blench R, & Dendo M. (2007). A History of fruits on the SE Asian MainLand. Paper presented at the EUREAA, Bourgon, 26 September 2006, p. 1–26. Cambridge, UK. http://www.rogerblench.info/RBOP.
[4] Chang FR (2001). Novel cytotoxic annonaceous acetogenins from Annona muricata. Journal National Productivity 64, 925–931. http://dx.doi.org/10.1021/np010035s.
[5] Doumas BT, Watson W, & Biggs HC (1971): Albumin standards and the measurement of serum albumin with bromocresol green. Clinica Chimica Acta 31, 87-96.http://dx.doi.org/10.1016/0009-8981(71)90365-2.
[6] Ekam VS, Johnson JT, Dasofunjo K, Odey MO &Anyahara SE (2012). Total protein, albumin and globulin levels following the administration of activity directed fractions of Vernonia amygdalina during acetaminophen induced hepatotoxicity in wistar rats. Annals of Biological Research 3(12), 5590-5594.
[7] Fawcett JK & Scotte JE (1960) " Arapid and Precise Method for Determination of Urea" Journal of Clinical Pathology 13, 156-157.http://dx.doi.org/10.1136/jcp.13.2.156.
[8] Gerecke AC & Sedlak D (2003). Precursors of N-nitrosodimethylamine in natural water. Environmental Science and Technolology 37(7), 1331-1336.http://dx.doi.org/10.1021/es026070i.
[9] Ismail RSA, El-Megeid AA & Abdel-Moemin AR (2009). Carbon tetrachloride-induced liver disease in rats: the potential effect of supplement oils with vitamins E and C on the nutritional status,†GMS German Medical Science 7, 1–10.
[10] Jendrassik L & Grof (1938): Total and Direct Bilirubin. Biochemistry 7297:81.
[11] Liaw CC, Chang FR, Lin CY, Chou CJ, Chiu HF, Wu MJ & Wu YC (2002). New cytotoxic monotetrahydrofuran annonaceous acetogenins from Annona muricata. Journal of National Productivity 65, 470–475.http://dx.doi.org/10.1021/np0105578.
[12] Li J, Xia Y &Kuter DJ (1999). Interaction of thrombopoietin with the platelet complements receptor in plasma: binding, internalization, stability and pharmacokinetics. British Journal Haematology 106, 345-349.http://dx.doi.org/10.1046/j.1365-2141.1999.01571.x.
[13] McLellan SA, McLellan DBL & Walsh TS (2003). Anaemia and red blood cell transfusion in the critically ill patient. Blood Rev 17: 195-208.http://dx.doi.org/10.1016/S0268-960X(03)00018-3.
[14] Morton J. (1973). Fruits of Warm Climates. Media. Winterville, NC: Creative Resource Systems, Inc pp.75–80.
[15] Muthulingam M (2002). Studies on the curative efficacy of Astercanthalongifoliaon carbon tetrachloride induced hepatotoxicity in rats. Ph.D.Thesis, Annamalai University.
[16] Patel VH. & Sail SS (2006).' Effect of different levels of dietary vitamin E On the biochemical alterations induced by N-dimethylnitrosamine intoxication. Journal of Pure & Applied Sciences 14, 53-61.
[17] Sadeek AE (2011). Protective effect of fresh Juice from red beetroot (Beta vulgaris L.) and radish (Raphanus sativusL.) against carbon tetrachloride - induced hepatotoxicity in rat models. African Journal of Biological Science 7(1), 69-84.
[18] Şener G, Şehirli AÖ & Ayanoğlu-Dülger G (2003). Protective effects of melatonin, vitamin E, and N-acetylcysteine against acetaminophen toxicity in mice: a comparative study. Journal Pineal Research 35, 61-8.http://dx.doi.org/10.1034/j.1600-079X.2003.00050.x.
[19] Tietz NW (1995). Clinical guide to laboratory tests. 3rd (Ed). W.B. Saunders Company Philadelphia. PA Pp518-519.
[20] U.S. EPA (2002). Integrated Risk Information System. Office of Research and Development (ORD), National Center for Environmental Assessment, Retrieved from: http: //www.epa.gov/ngispgm3/iris /search.htm.
[21] Usunobun U (2014). Protective effects of Annona muricata ethanolic leaf extract against Dimethylnitrosamine (DMN)-Induced Hepatotoxicity. IOSR Journal of Pharmacy & Biological Sciences 9(4):01-06.
[22] Usunobun U, Okolie NP, Anyanwu OG, Adegbegi AJ & Egharevba ME (2015). Phytochemical screening and proximate composition of Annona muricata leaves. European Journal of Botany, Plant Science & Phytology 2(1), 18-28.
[23] Usunobun U & Okolie NP (2015). Phytochemical analysis and mineral composition of Annona muricata leaves. International Journal of Research & Current Development1(1): 38-42.
[24] Usunobun U, Okolie PN & Eze GI (2015). Modulatory effect of ethanolic leaf extract of Annona muricata pre-treatment on liver damage induced by Dimethylnitrosamine (DMN) in rats. British Journal of Pharmaceutical Research8(3): 1-9.http://dx.doi.org/10.9734/BJPR/2015/19841.
[25] Vogel AI (1971). A textbook of practical organic Chemistry including qualitative organic analysis. Longman group limited, London. Pp 426.
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How to Cite
Usunobun, U., & Okolie, N. (2016). Effect of Annona muricata pre-treatment on liver synthetic ability, kidney function and hematological parameters in dimethylnitrosamine (DMN)-administered rats. International Journal of Medicine, 4(1), 1-5. https://doi.org/10.14419/ijm.v4i1.5709Received date: 2015-12-29
Accepted date: 2016-01-18
Published date: 2016-01-19