Detection, Isolation and Antimicrobial Testing of Listeria monocytogenes in Chicken from Supermarket

  • Authors

    • John Yew Huat Tang
    • Aisyah Amirah Ismail
    • Noor Afiza Badaluddin
    2018-12-29
    https://doi.org/10.14419/ijet.v7i4.43.25829
  • Listeria monocytogenes, real time PCR, antibiotic resistance, chicken, supermarket.

  • Abstract

    Listeria monocytogenes play a vital role in causing an infrequent and sometimes deadly foodborne disease known as Listeriosis. In this study, the presence of L. monocytogenes in chicken at supermarket and the antimicrobial susceptibility of the pathogen were determined. Detection of L. monocytogenes in chickens from supermarket was carried out using real time PCR and isolation of L. monocytogenes by plating onto PALCAM agar. The L. monocytogenes isolates were tested against 14 antibiotics for resistance determination using disc diffusion method. From 56 chicken samples tested, 35.7 % of the samples were detected contained the pathogen using real time PCR. Only 15 samples  (26.8%) were positive for L. monocytogenes isolates. The isolates showed high resistance towards Penicillin G (100%), Ampicillin (93%), Rifampicin (93%), Ceftazidime (87%), Erythromycin (87%), and Tetracycline (80%). All the isolates were found to have the Multiple Antibiotic Resistance (MAR) index more than 0.2 which indicates multiple resistance towards antibiotics. In conclusion, L. monocytogenes was found in the chicken at supermarket and the isolates were found to be highly resistance towards multiple antibiotics. Thus, chicken meat must be properly cooked before consumption to prevent foodborne infection.

     

  • References

    1. [1] Charlier, C., Perrodeau, É., Leclercq, A., Cazenave, B., Pilmis, B., Henry, B. & Zumbo, C. (2017) Clinical features and prognostic factors of listeriosis: the MONALISA national prospective cohort study. The Lancet Infectious Diseases 17(5), 510–519.

      [2] Moura, G.F., Sigarini, C.D.O., Eustáquio, E. & Figueiredo, D.S. (2016) Listeria monocytogenes in Chicken Meat. Journal of Food and Nutrition Research 4(7), 436–441.

      [3] Department of Veterinary Services. 2018. Malaysia: per capita consumption of livestock products 2007-2016. Accessed on 22 October 2018. http://www.dvs.gov.my/dvs/resources/user_1/DVS%20pdf/Perangkaan%202015%202016/page_10.pdf.

      [4] Zoz, F., Grandvalet, C., Lang, E., Iaconelli, C., Gervais, P., Firmesse, O. & Beney, L. (2017) Listeria monocytogenes ability to survive desiccation: Influence of serotype, origin, virulence, and genotype. International Journal of Food Microbiology 248, 82–89.

      [5] Goh, S.G., Kuan, C.H., Loo, Y.Y., Chang, W.S., Lye, Y.L., Soopna, P., Tang, J.Y.H., Nakaguchi, Y., Nishibuchi, M., Afsah-Hejri, L. & Son, R. (2012) Listeria monocytogenes in retailed raw chicken meat in Malaysia. Poultry Science 91(10), 2686–2690.

      [6] CLSI. (2017) Performance standards for antimicrobial susceptibility testing. 27th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 27th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute.

      [7] Marian, M.N., Aminah, S.M.S., Zuraini, M.I., Son, R., Maimunah, M., Lee, H.Y. & Elexson, N. (2012) MPN-PCR detection and antimicrobial resistance of Listeria monocytogenes isolated from raw and ready-to-eat foods in Malaysia. Food Control 28(2), 309–314.

      [8] Uyttendaele, M., De Troy, P. & Debevere, J. (1999) Incidence of Salmonella, Campylobacter jejuni, Campylobacter coli, and Listeria monocytogenes in poultry carcasses and different types of poultry products for sale on the Belgian retail market. Journal of Food Protection 62(7), 735–740.

      [9] Alsheikh, A.D.I., Mohammed, G.E. & Abdalla, M.A. (2013) Isolation and identification of Listeria monocytogenes from retail broiler chicken ready to eat meat products in Sudan. International Journal of Animal and Veterinary Advances 5(1), 9–14.

      [10] Praakle, K. (2016) Campylobacter spp. and Listeria monocytogenes in Poultry Products In Estonia. PhD Thesis, Estonian University of Life Sciences.

      [11] Reiter, M.G.R., Bueno, C.M.M., López, C. & Jordano, R. (2005) Occurrence of Campylobacter and Listeria monocytogenes in a poultry processing plant. Journal of Food Protection 68(9), 1903–1906.

      [12] Arumugaswamy, R. K., Gulam R.R.A. & Siti Nadzriah, A.H. (1994) Prevalence of Listeria monoctogenes in foods in Malaysia. International Journal of Food Microbiology 23(1), 117–121.

      [13] Bergholz, T. M., Bowen, B., Wiedmann, M. & Boor, K.J. (2012) Listeria monocytogenes shows temperature-dependent and -independent responses to salt stress, including responses that induce cross-protection against other stresses. Applied and Environmental Microbiology 78(8), 2602–2612.

      [14] Ceylan, Z.G., Demikaya, A.K. & Adiguzel, G. (2008) Incidence of listeria monocytogenes in retail chicken meat and establishing relationship with some bacteria by logistic regression. Journal of Food Quality 31, 121-130.

      [15] Charpentier, E. & Courvalin, P. (1999) Antibiotic resistance in Listeria spp. Antimicrobial Agents and Chemotherapy 43 (9), 2103-2108.

      [16] Jang, S.S., Choo, E., Han, K., Miyamoto, T., Heu, S. & Ryu, S. (2006) Antibiotic resistance and genetic diversity of Listeria monocytogenes isolated from chicken carcasses in Korea. Journal of Microbiology and Biotechnology 16(8), 1276–1284.

      [17] Levy, S.B. & Bonnie, M. (2004) Antibacterial resistance worldwide: Causes, challenges and responses. Nature Medicine 10(12S), S122–S129.

      [18] Pesavento, G., Ducci, B., Nieri, D., Comodo, N. & Lo Nostro, A. (2010) Prevalence and antibiotic susceptibility of Listeria spp. isolated from raw meat and retail foods. Food Control 21(5), 708–713.

  • Downloads

  • How to Cite

    Yew Huat Tang, J., Amirah Ismail, A., & Afiza Badaluddin, N. (2018). Detection, Isolation and Antimicrobial Testing of Listeria monocytogenes in Chicken from Supermarket. International Journal of Engineering & Technology, 7(4.43), 113-116. https://doi.org/10.14419/ijet.v7i4.43.25829

    Received date: 2019-01-13

    Accepted date: 2019-01-13

    Published date: 2018-12-29