Determination of Nitrogen Fixing Capacity of Bacteria Isolated from the Rhizosphere of Acacia Mangium from the BRIS Soil of Tembila, Besut, Terengganu, Malaysia

  • Authors

    • Zakiah Mustapha
    • Radziah Othman
    • Nur Laili Samsurrijal
    • Nashriyah Mat
    • Abd Jamil Zakaria
    • Nor Hasima Mahmod
    2018-12-29
    https://doi.org/10.14419/ijet.v7i4.43.25836
  • ARA, bacteria, biofertilizer BRIS soil, nitrogen, rhizosphere.

  • Abstract

    The present study was conducted to isolate beneficial bacteria with nitrogen fixing potential from the rhizosphere of Acacia mangium plant. The plant grows wildly on the untouched area of problematic BRIS soil in Tembila, Terengganu. A total of 24 bacterial isolates were successfully sequestered from the soil samples using the Burk’s nitrogen free medium. Based on the preliminary morphology screening, eight bacterial isolates were selected and their morphological and biochemical characteristics were recorded.  Three bacterial isolates namely UA1, UA6 and UAA2 were selected as final species for antibiotic sensitivity test and Acetylene Reduction Assay (ARA). All three bacterial isolates showed a high amount of nitrogenase activity in ARA with UA6 produced the highest and significant amount of ethylene with 18.55 nmolC2H4mL-1h-1. UA1 and UAA2 showed almost similar amount of ethylene with 17.10 and 17.00 nmolC2H4mL-1h-1 respectively. The isolated bacteria characteristics through all the test showed that these bacteria have potential to be explore for biofertilizer production industry.

     

     

  • References

    1. [1] Suraj Singh, R.K., Mazumber, P.B., Bhattacharjee, M.K. & Sharma, G. (2013) Determination of nitrogen fixing capacity of bacteria isolated from the rhizosphere soil of Crotolaria pallida from the valley district of Manipur, India. Journal of Pharmacy and Biological Sciences 8(4), 20-24.

      [2] Dalton, H. (1980) The cultivation of diazotrophic microorganisms. In: Methods for evaluating biological nitrogen fixation, (Bergersen, F.J., ed.), p. 13–64. Chichester: John Wiley & Sons.

      [3] Stella, M. & Suhaimi, M. (2010) Selection of suitable growth medium for free-living diazotrophs isolated from compost. Journal of Tropical Agriculture and Food Science 38(2), 211-219.

      [4] Roslan, I., Shamshuddin, J., Fauziah, C.I. & Anuar, A.R. (2011) Fertility and suitability of the Spodosols formed on sandy beach ridges interspersed with swales in the Kelantan - Terengganu Plains of Malaysia for kenaf production. Malaysian Journal of Soil Science 15, 1–24.

      [5] Usman, M.I., Edi Armanto, H.M. & Adzemi, M.A. (2013) Performance of BRIS Soils Genesis and Classification in Terengganu, Malaysia. Journal of Biology, Agriculture and Healthcare 3(20), 86-92.

      [6] Roslan, I., Shamsuddin, C.I. & Anuar, A.R. (2010) Occurrence and properties of soils on sandy beach ridges in the Kelantan-Terengganu Plains, Peninsular Malaysia. Catena 83(2010), 55-63.

      [7] Orwa, C., Mutua, A., Kindt, R., Jamnadass, R. & Anthony, S. (2009) Agroforestree Database: a tree reference and selection guide version 4.0. http://www.worldagroforestry.org/sites/treedbs/treedatabases.asp.

      [8] Lim, S.C., Gan, K.S. & Tan, Y.E. (2011) Properties of Acacia mangium Planted in Peninsular Malaysia. ITTO Project on Improving Utilization and Value Adding of Plantation Timbers from Sustainable Sources in Malaysia Project No. PD 306/04(1). Forest Research Institue Malaysia. Percetakan Warni Sdn. Hbd. Batu Caves. p 1-2.

      [9] Ho, K.S., Hamdan, H., Tan, Y.E. & Mohd. Shukari, M. (1999) Acacia mangium for timber and a case study of utilisation. Paper presented at the Fifth Conference on Forestry and Forest Products, 1999 Series. Utilization of Plantation Timber: Sentang: Potential Timber for The future , 20 April 1999. Auditorium FRIM, p 9.

      [10] Bano, S.A. & Iqbal, S.M. (2016), Biological nitrogen fixation to improve plant growth and productivity. International Journal of Agriculture Innovation and Research 4(4), 596-599.

      [11] John, G.H., Kreig, N.R., Smeath, H. A. P., Staley, J.T. & Williams, S.T. (1994) Bergey’s Manual of Determinative of Bacteriology, 9th ed. (pp. 605-702). Baltimore, U.S.A.: The Williams and Wilkins Co.

      [12] Cappucino, J.G. & Sherman, N. (2005) Microbiology- a laboratory manual, 7th ed. (pp. 161-162). San Francisco, CA: Pearson Education, Inc.

      [13] Bauer, A.W., Kirby, W.M.M., Sherris, J.C. & Turck, M. (1966) Antibiotic Susceptibility Testing by Standardized Single Disk Method. American Journal of Clinical Pathology 45, 493-496.

      [14] Hardy, R.W.F., Holsten, R.D., Jackson, E.K & Burns, R.C. (1968) The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation, Plant Physiology 43, 1185-1207.

      [15] Somasegaran, P. & Hoben, H.J. (1985) Methods in legume-rhizobium technology, University of Hawaii, NifTAl Project and MIRCEN.

      [16] Monokoane, P.C., Mekbib, S.B. & Haiying, L. (2016) Isolation, molecular characterization and growth promoting activity of free living diazotrophs screened from soils of Lesotho. Academia Journal of Agricultural Research 4(2), 45-52.

      [17] Chelikani, P., Fita, I. & Loewen, P.C. (2004) Diversity of structures and properties among catalases. Cellular and Molecular Life Sciences 61(2), 192-208.

      [18] Marschner, P., Yang, C.H., Lieberei, R. & Crowley, D.E. (2001) Soil and plant specific effects on bacterial community composition in the rhizosphere. Soil Biology and. Biochemistry 33, 1437–1445.

      [19] Kumar, S. (2015) Plant Ureases: Physiological significance, role in agriculture and industrial applications - A review. South Asian Journal of Food Technology and Environment 1(2), 105-115.

      [20] Lee, J.H., Wood, T.K. & Lee, J. (2015) Roles of Indole as an Interspecies and Inter kingdom Signaling Molecule. Trends in Microbiology 23(11), 707-18.

      [21] Abdul Halim, N.B. (2009) Effects of using enhanced biofertilizer containing N-fixer bacteria on patchouli growth. Thesis. Faculty of Chemical and Natural Resources Engineering University Malaysia Pahang. p. 145.

      [22] Gupta, A.K. (2004) The complete technology book on biofertilizers and organic farming. National Institute of Industrial Research Press. India.

      [23] Smail, F. (2000) Antibiotic Susceptibility Resistance and Testing: An Overview. Canadian Journal of Gastroenterology and Hepatology 14(10), 871-875.

      [24] Kucey, R.M.N. (1988) Alteration of size of wheat root systems and nitrogen fixation by associative nitrogen-fixing bacteria measured under field condition. Canadian Jounal of Microbiology 34, 735-739.

      [25] Boddey, R.M., de Oliveira, O.C., Urquiaga, S., Reis, V.M., Olovares, F.L., Baldani, V. L.D. & Dobereiner, J. (1995) Biological nitrogen fixation associated with sugar cane and rice: Contribution and prospects for improvements. Plant and Soil 174, 195-209.

      [26] Watanabe, I., Barraquio, W.L., De Guzman, M.R. & Cabrera, D.A. (1979) Nitrogen-fixing (acetylene reduction) activity and population of aerobic heterotrophic nitrogen-fixing bacteria associated with wetland rice. Applied and Environmental Microbiology 37(5), 813-819.

      [27] Różycki, H., Dahm, H., Strzelczyk, E. & Li, C.Y. (1999) Diazotrophic bacteria in root-free soil and in the root zone of pine (Pinus sylvestris L.) and oak (Quercus robur L.). Applied Soil Ecology 12(3), 239–250.

      [28] Balouei, F., Jaafar, H.Z.E., Khalatbari, A.M., Othman, R., Ibrahimi, M., Elmahdi, S. & Abdullah, N. (2016) The response and recovery of N2-fixation activity in soybean (Glycine max) inoculated with PGPR to water deficit at onset of pod and onset of seed stages. International Journal of Advanced Biotechnology and Research 7(3), 1309-1321.

      [29] Hara, S., Hashidoko, Y., V., Desyatkin, R., Hatano, R. & Tahara, S. (2009) High Rate of N2 Fixation by East Siberian Crophilic Soil Bacteria as Determined by Measuring Acetylene Reduction in Nitrogen- Poor Medium Solidified with Gellam Gum. Applied and Environmental Microbiology 75 (9), 2811-2819.

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  • How to Cite

    Mustapha, Z., Othman, R., Laili Samsurrijal, N., Mat, N., Jamil Zakaria, A., & Hasima Mahmod, N. (2018). Determination of Nitrogen Fixing Capacity of Bacteria Isolated from the Rhizosphere of Acacia Mangium from the BRIS Soil of Tembila, Besut, Terengganu, Malaysia. International Journal of Engineering & Technology, 7(4.43), 140-144. https://doi.org/10.14419/ijet.v7i4.43.25836

    Received date: 2019-01-13

    Accepted date: 2019-01-13

    Published date: 2018-12-29